Effect of sperm concentration on characteristics and fertilization capacity of rooster sperm frozen in the presence of the antioxidants catalase and vitamin E

Moghbeli, Morteza; Kohram, Hamid; Zare-Shahaneh, Ahmad; Zhandi, Mahdi; Sharideh, Hossein; Sharafi, Mohsen

doi:10.1016/j.theriogenology.2016.03.038

Cited by 25 publications

(10 citation statements)

References 39 publications

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“…The vaginal segment of a hen’s oviduct regulates spermatozoa, and only motile spermatozoa will traverse the vagina and enter the hen’s sperm storage tubules [7]. Motility of spermatozoa is a primary determinant of fertility in fowl [8], turkeys [9], Venda chickens [10], and roosters [11,12]. A graded influence was predicted between fertility and spermatozoa motility when fertility was plotted as a function of spermatozoa motility.…”

Section: Introductionmentioning

confidence: 99%

A diluent containing coconut water, fructose, and chicken egg yolk increases rooster sperm quality at 5°C

Rochmi

Sofyan

2019

Vet World

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Aim: The present study was conducted to evaluate the quality of rooster sperm at 5°C after treatment with a diluent containing coconut water, fructose, and chicken egg yolk and stored the semen sample at 5°C. Materials and Methods: Ten semen samples from 10 healthy roosters were subjected to four different treatments. For the treatments, 0.2 ml fresh semen with a sperm concentration of 5.2X109 cell/ml was mixed with T0 (no diluent), T1 (0.34 ml coconut water and 6 μl fructose), T2 (0.274 ml coconut water, 0.12 ml egg yolk, and 6 μl fructose), and T3 (0.34 ml egg yolk and 6 μl fructose) solutions. Each treated solution was stored at 5°C and evaluated both macroscopically and microscopically. Macroscopically, semen volume, pH, and sperm concentration were evaluated. The microscopic sperm characteristics examined included total motility (i.e., rapid, medium, or slow), progressive and non-progressive motility, viability, and spermatozoa abnormalities noted at different storage times. The results showed that spermatozoa motility was under 40%. Results: The results indicated that sperm viability significantly affected (p<0.05). The highest mean value of sperm viability on day 7 of storage was found after treatment with the T2 solution (46.100±0.5677%). Similarly, spermatozoa abnormalities were significantly lower after treatment with the T2 solution (6.680±1.702%). Conclusion: The addition of a diluent containing coconut water, egg yolk, and fructose helped in the better preservation spermatozoa motility, as well as viability for up to 7 days when the semen samples were stored at 5°C.

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Section: Introductionmentioning

confidence: 99%

A diluent containing coconut water, fructose, and chicken egg yolk increases rooster sperm quality at 5°C

Rochmi

Sofyan

2019

Vet World

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“…However, in other species this is not the case. A number of reports on mammalian and avian spermatozoa [12,[32][33][34][35] have studied the effects of sperm concentration on the quality of semen, particularly following freezing and thawing. There is a general consensus that a greater concentration of spermatozoa results in the reduction of sperm motility and viability.…”

Section: Discussionmentioning

confidence: 99%

Dynamic assessment of human sperm DNA damage II: the effect of sperm concentration adjustment during processing

Tvrdá

Arroyo

Ďuračka

et al. 2019

J Assist Reprod Genet

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Purpose To evaluate the effect of sperm concentration adjustment in human ejaculates on the sperm DNA quality and longevity. Methods Semen samples were obtained from 30 donors with a normal spermiogram. Following centrifugation, the sperm pellet was resuspended in PBS, and the sperm concentration adjusted to 200, 100, 50, 25, 12, and 6 × 10 6 /mL. Each set of samples was incubated at 37 °C for 24 h, and the sperm DNA damage was assessed using the chromatin-dispersion test following 0 h, 2 h, 6 h, and 24 h of incubation. Results Sperm DNA fragmentation (SDF) did not differ between the selected experimental conditions at T0; however, Kaplan-Meier estimates for survival showed significant differences with respect to the dilution and time (all P values were smaller than .001). DNA fragmentation in semen samples adjusted to 200 × 10 6 /mL was approximately 3.3 times higher when compared to samples containing 25 × 10 6 /mL and 3.9 higher in comparison with samples adjusted to 12 × 10 6 /mL following 2 h of in vitro incubation. Although there was evidence of individual variation in SDF during the incubation period, the general finding was that lower sperm concentrations resulted in a slower rate of DNA fragmentation. Conclusions Incubation of spermatozoa for ART purposes should be done following a concentration adjustment below 25 × 10 6 / mL in order to avoid a higher susceptibility of the sperm DNA molecule towards fragmentation.

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“…Vitamin E is a cellular stabilizer of unsaturated lipids against oxidative deterioration, and hence, it maintains the structural and functional integrity at the subcellular level [161,162]. In general, Vitamin E is the primary component of the antioxidant system in sperm cells [163,164].…”

Section: Vitamin Ementioning

confidence: 99%

“…In general, Vitamin E is the primary component of the antioxidant system in sperm cells [163,164]. Furthermore, adding Vitamin E to Tris-egg yolk extenders at 60 and 120 μM provides higher integrity to the plasma membrane, mitochondria, and kinematic parameters of sperm cells of rams [165], roosters [162], and bulls [164] post-cryopreservation.…”

Section: Vitamin Ementioning

confidence: 99%

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Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders

Bustani

Baiee

2021

Vet World

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Reproduction is fundamental for all living things as it ensures the continued existence of a species and an improved economy in animal husbandry. Reproduction has developed since history, and diverse processes, such as artificial insemination and in vitro fertilization, have been developed. Semen extenders were discovered and developed to protect sperm from harmful factors, such as freeze and osmotic shock, oxidative stress, and cell injury by ice crystals. Semen extenders preserve sperm by stabilizing its properties, including sperm morphology, motility, and viability and membrane, acrosomal, and DNA integrity. Therefore, semen extenders must provide a favorable pH, adenosine triphosphate, anti-cooling and anti-freeze shock, and antioxidant activity to improve semen quality for fertilization. Hence, this review provides precise data on different semen extenders, preservative mechanisms, and essential additives for semen extenders in different animals.

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Effect of sperm concentration on characteristics and fertilization capacity of rooster sperm frozen in the presence of the antioxidants catalase and vitamin E

Cited by 25 publications

References 39 publications

A diluent containing coconut water, fructose, and chicken egg yolk increases rooster sperm quality at 5°C

A diluent containing coconut water, fructose, and chicken egg yolk increases rooster sperm quality at 5°C

Dynamic assessment of human sperm DNA damage II: the effect of sperm concentration adjustment during processing

Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders

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