2007
DOI: 10.1111/j.1439-0531.2006.00744.x
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Effect of Sperm Cryopreservation and Supplementing Semen Doses with Seminal Plasma on the Establishment of a Sperm Reservoir in Gilts

Abstract: Frozen-thawed (FT) boar sperm have a reduced fertile life, due in part to a capacitation-like status induced by cooling. Reversal of this cryocapacitation in vitro by exposure to boar seminal plasma (SP) has been demonstrated. The objective of these studies was to determine the effect of SP on the ability of FT sperm to create an oviductal sperm reservoir following artificial insemination (AI). In Experiment one, 35 pre-pubertal gilts were injected (IM) with 400 IU eCG plus 200 IU hCG to induce oestrus. At det… Show more

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Cited by 20 publications
(22 citation statements)
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“…In contrast, insemination of thawed sperm resulted in very small sperm populations in the UTJ (Abad et al. ). Multiple inseminations are employed to compensate for the lack of knowledge of when ovulation occurs.…”
Section: Introductionmentioning
confidence: 99%
“…In contrast, insemination of thawed sperm resulted in very small sperm populations in the UTJ (Abad et al. ). Multiple inseminations are employed to compensate for the lack of knowledge of when ovulation occurs.…”
Section: Introductionmentioning
confidence: 99%
“…Although the in vitro analyses attempt to mimic the conditions that the spermatozoa would encounter in vivo, fertilisation is a complex process in which the spermatozoa are sequentially exposed to various environments along the female reproductive tract before they encounter and fertilise the oocytes (Rodriguez-Martinez 2007). Once the spermatozoa are deposited in the female genital tract, a functional sperm reservoir (SR) is established in the ampullary-isthmic junction (Rodriguez-Martinez 2007) and this reservoir is markedly reduced when frozen-thawed spermatozoa are used (Abad et al 2007). The main function of the SR is to maintain a selected population of viable spermatozoa for an extensive period of time (up to 36 h in pigs; Smith 1998), which will better coordinate sperm capacitation at the time of ovulation (for a review, see Petrunkina et al 2007).…”
Section: Discussionmentioning
confidence: 99%
“…Whether the difference we observed between treatments in vitro has implications for fertility in vivo should be examined in future studies. Because cryopreservation markedly reduces the ability of spermatozoa to establish a sperm reservoir in the oviduct (Abad et al 2007), treatments that improve the association between spermatozoa and oviductal epithelial cells could have a positive effect on the fertilising ability of the spermatozoa.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Many studies have shown that the fertility of FTS is reduced when using comparable numbers of liquid extended sperm (Waberski et al, 1994;Johnson et al, 2000;Roca et al, 2011). The decline in fertility following insemination has been attributed to a reduced ability of FTS to form a reservoir similar to that for liquid extended semen (Pursel et al, 1978;Abad et al, 2007).…”
Section: Introductionmentioning
confidence: 97%