Effect of sperm selection method by cumulus oophorus complexes and conventional sperm preparation method on sperm quality and DNA fragmentation for assisted reproduction techonology

Naknam, Wasin; Salang, Lingling; Sothornwit, Jen; Amnatbuddee, Siriruthai; Seejorn, Kanok; Pongsritasana, Thanida; Sukkasame, Sineenart

doi:10.1016/j.ejogrb.2019.10.004

Cited by 8 publications

(12 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They postulated that this approach may improve the ICSI outcomes 37 . The competency of this technique in selecting spermatozoa with better motility was reinforced again by Naknam et al., who also showed lower rate of sperm DNA fragmentation in selected sperm population 30 . In 2018, Wang et al.…”

Section: Discussionmentioning

confidence: 99%

“…Once ovulation is induced, due to oocyte‐induced secretion of extra cellular matrix by cumulus cells, the cumulus cells surrounding the oocyte expand and allow spermatozoa with certain characteristics to pass the cumulus cells and penetrate the zona pellucida 18 . One of the most popular novel sperm selection approaches is the ability of spermatozoa to attach to surface‐bounded‐hyaluronic acid, one of the components of the extra cellular matrix 30–32 . Despite studies showing HA has the ability to select spermatozoa with intact DNA, 30–32 based on recent Cochrane study and previous research studies, 33 ‐– 35 it has been stated that there may be little or no difference for pregnancy between HA‐ICSI vs. conventional ICSI, but there is strong evidence for reduced miscarriage rates.…”

Section: Discussionmentioning

confidence: 99%

“…18 One of the most popular novel sperm selection approaches is the ability of spermatozoa to attach to surfacebounded-hyaluronic acid, one of the components of the extra cellular matrix. [30][31][32] Despite studies showing HA has the ability to select spermatozoa with intact DNA, [30][31][32] based on recent Cochrane study and previous research studies, 33--35 it has been stated that there may be little or no difference for pregnancy between HA-ICSI vs.…”

Section: Discussionmentioning

confidence: 99%

“…37 The competency of this technique in selecting spermatozoa with better motility was reinforced again by Naknam et al, who also showed lower rate of sperm DNA fragmentation in selected sperm population. 30 In 2018, Wang et al modified this technique by allowing spermatozoa to pass horizontal columns containing cumulus cell in ICSI petri dishes, using sibling oocytes. 20 They showed that spermatozoa which penetrated the cumulus cell result in higher fertilization rate and formation of top-quality blastocysts, but they observed no difference in implantation rate and pregnancy rate with their conventional IVF group.…”

Section: Discussionmentioning

confidence: 99%

“…One of the most popular novel sperm selection approaches is the ability of spermatozoa to attach to surface‐bounded‐hyaluronic acid, one of the components of the extra cellular matrix 30–32 . Despite studies showing HA has the ability to select spermatozoa with intact DNA, 30–32 based on recent Cochrane study and previous research studies, 33 ‐– 35 it has been stated that there may be little or no difference for pregnancy between HA‐ICSI vs. conventional ICSI, but there is strong evidence for reduced miscarriage rates. Considering these limitations, we feel there is room to explore more physiological and integrated methods of sperm selection.…”

Section: Discussionmentioning

confidence: 99%

See 4 more Smart Citations

Single‐blind clinical trial: Sperm selection based on capacity to pass through cumulus oophorous column improves ICSI outcomes

et al. 2021

View full text Add to dashboard Cite

Background: Sperm selection procedures for future strategies that aim to select normal spermatozoa with intact DNA to improve intracytoplasmic sperm injection (ICSI) outcomes are in early developing stage. Objectives:The objective is to find out whether the sperm selection procedure based on the ability of spermatozoa to traverse the cumulus cells could improve clinical outcomes of ICSI technique in infertile couples with male factor etiology. Materials and methods:For this single-blind clinical trial, mature metaphase II oocytes were retrieved from 150 couples with male factor infertility, male age lower than 45 years and female age under 38 years. These couples were divided into two groups.In control group (n = 75), spermatozoa processed by density gradient centrifugation (DGC) were used to inject the oocytes. In the study group (n = 75), the oocytes were divided into sibling groups. In one sibling group (DGC), the oocytes were inseminated with DGC-processed spermatozoa while in the other group (DGC-CC), they were inseminated with DGC-processed spermatozoa that passed cumulus oophorous column.Results: Mean fertilization and embryo quality were significantly higher in DGC-CC group compared to DGC and control group. In addition, mean of chemical pregnancy (52.27% vs. 34.14%; p = 0.05), clinical pregnancy based on sac (52.27% vs. 32.92%; p = 0.03), clinical pregnancy with heart beat (52.27% vs. 25.60%; p = 0.003) and ongoing pregnancy (43.18% vs. 21.95%; p = 0.02) rates were significantly higher in DGC-CC group compared to control group. Conclusion:Sperm selection based on integrated systems such as DGC and ability to pass through cumulus oophorous column could improve clinical outcomes of ICSI in couples with male factor infertility.

show abstract

Section: Discussionmentioning

confidence: 99%