Effect of squalene on superoxide anion generation induced by a skin irritant, lauroylsarcosine

Aioi, Akihiro; Shimizu, Tatsuo; Kuriyama, Kiyoshi

doi:10.1016/0378-5173(94)00190-g

Cited by 28 publications

(15 citation statements)

References 22 publications

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“…The suppression of 02-production is an especially important finding because the 02-scavenger SOD decreased LS-induced erythema as reported previously (10), indicating that 02-is a major mediator responsible for the skin irritation, because some reports have demonstrated that 02-plays a predominant role in the progress of skin inflammations (11,12). Hence, vitamin E is considered to alleviate the erythema by suppressing 02-production from keratinocytes at an early phase of LS-induced irritation.…”

Section: Discussionmentioning

confidence: 73%

Effect of Vitamin E on Keratinocyte-Modulation Induced by Lauroylsarcosine

Shimizu

Aioi

Horiguchi

et al. 1995

Japanese Journal of Pharmacology

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ABSTRACT-The effect of vitamin E on the modulation of keratinocytes was studied in rats. A 1°10 lauroylsarcosine (LS) ointment caused skin erythema with keratinocyte-damage. A 30% vitamin E ointment markedly alleviated this erythema and protected keratinocytes from cell damage. Vitamin E (100 pg/ml) was also effective on LS (7.5 pg/ml)-induced proliferative reduction of cultured keratinocytes. On the other hand, ointment containing superoxide dismutase (SOD) (99,000 U/g) decreased the LS-induced erythema, suggesting that superoxide anion (02) produced from keratinocytes play an important role in the skin irritation. Indeed, LS induced 02-production from cultured keratinocytes. The 02-was significantly reduced by vitamin E and SOD, although vitamin E had no effects on 02-production in a xanthine-xanthine oxidase system, unlike the effect observed with SOD. These results indicate that vitamin E is an inhibitor of keratinocyte-modulation.

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Section: Discussionmentioning

confidence: 73%

Effect of Vitamin E on Keratinocyte-Modulation Induced by Lauroylsarcosine

Shimizu

Aioi

Horiguchi

et al. 1995

Japanese Journal of Pharmacology

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“…Epidemiological studies have revealed that squalene can effectively inhibit chemically induced colon, lung and skin tumorigenesis in rodents and is an effective chemopreventive agent compared to other dietary food ingredients (Aioi et al 1995;Budiyanto et al 2000;Smith 2000). In mice, supplementation of squalene-containing diets has resulted in marked increases in cellular and nonspecific immune functions in a dose-dependent manner (Kelly 1999).…”

Section: Introductionmentioning

confidence: 98%

Enhanced production of squalene in the thraustochytrid Aurantiochytrium mangrovei by medium optimization and treatment with terbinafine

Fan

Aki

Chen

et al. 2010

World J Microbiol Biotechnol

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Squalene is an effective chemopreventive agent in reducing the incident of coronary heart disease and cancer. It is also a strong antioxidant used extensively in the food and cosmetic industries. Microbial sources of squalene are being explored in recent years. The objective of this study is to increase the squalene content and yield in the thraustochytrid, Aurantiochytrium mangrovei FB3 through medium optimization and the treatment with terbinafine, an inhibitor of squalene monooxygenase in the sterol biosynthetic pathway. The highest biomass concentration of 21.2 g l(-1) was obtained at a glucose concentration of 60 g l(-1), while the highest specific growth rate of 0.077 h(-1) and the growth yield coefficient of 0.44 g g(-1) based on glucose were achieved at a lower glucose concentration (30 g l(-1)). The addition of terbinafine led to a slight inhibition of cell growth whereas an obvious increase in squalene content was observed at terbinafine concentrations of 10 and 100 mg l(-1), which corresponded to an increase of 36 and 40% in squalene content, respectively compared to the control. The addition of terbinafine was thus effective in inducing the accumulation of squalene in A. mangrovei. This study not only demonstrated the production potential of squalene by A. mangrovei, but also provided novel information on the accumulation effect of terbinafine on the biosynthesis of an essential intermediate involved in sterol metabolic pathway.

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“…Squalene is a scavenger of reactive oxygen species and free radicals; therefore, it is frequently used in the cosmetic industry as an antioxidant, emollient and moisturizing agent (Kohno Y, 1995 ). Squalene has also been reported to cure chemically induced lung, colon, and skin tumorigenesis (Akihiro A, 1995;Arief B, 2000;Newmark, 1997;Rao CV, 1998;Smith TJ, 2000;Smith G., 1998). Squalene also has cardioprotective (Aguilera Y, 2005) and radioprotective effects (Heidi MS, 1993), boosts the nonspecific and cellular immune response (Kelly GS, 1999 ) and effectively reduces serum cholesterol (Miettinen TA, 1994).…”

Section: Introductionmentioning

confidence: 99%