Effect of Ssanghwa-tang Extract on Antioxidant and Anti-aging Enzyme Activities

Abstract

Objectives : The present study was designed to investigate effects of Ssanghwa-tang (Shuānghuā-gēng) on oxidation/reduction reaction-related and aging-related enzymes in vitro.Methods : We performed MTT assay, collagenase inhibition assay, elastase inhibition assay, tyrosinase inhibition assay, DPPH free radical scavenging assay, SOD-like activity and xanthine oxidase (XO) inhibition assay. Results : The 50% ethanol (EtOH) extract of Ssanghwa-tang (SHT) showed 55% inhibition of collagenase activity, and 42% inhibition of elastase activity at 1 mg/ml concentration. Also it's treatment showed 18% inhibition of tyrosinase activity, to relate whitening effect, at the same dose of 50% ethanol extract of SHT. Antioxidant activities were determined by DPPH radical scavenging, XO inhibiting activity and SOD-like activity. These scavenging, XO-inhibiting and SOD-like activities were measured in 80%, 75%, and 28% inhibitions, respectively, at a 1 mg/ml treated dose, compared to those of control. The inhibitory effects of 50% EtOH extract on aging and oxidation-related enzyme activities were higher than those of water extract and 95% EtOH extract. Conclusions : Taken together, our findings suggest that the SHT has potential and applicable benefits for development of cosmetics to have anti-aging (anti-wrinkle and whitening) and anti-oxidation functions.

Correlation of anti-wrinkling and free radical antioxidant activities of Areca nut with phenolic and flavonoid contents

“…Collagen, a major component of the extracellular matrix, is a matrix protein produced by fibroblasts in the skin. The regulation of biosynthesis and degradation of collagen is a core metabolism process of skin aging and skin wrinkle formation (Park et al, 2012). The results of the collagenase enzyme inhibition assay using Areca nut extracts showed better inhibition of collagenase activity than EGCG in all extracts.…”

Assessment of genotoxicity of Ssanghwa-tang, an herbal formula, by using bacterial reverse mutation, chromosome aberration, and in vivo micronucleus tests

“…In recent studies, SHT has been reported to possess many functions, such as protection against acute hepatotoxicity, anti-melanogenic activity, and inhibitory effects on bone loss [9][10][11] . It has also been reported to exert analgesic, anti-convulsant, anti-oxidative, anti-aging and anti-inflammatory effects [12][13][14] . A recent study showed that SHT exerted anti -inflammatory effects on cigarette smoke-induced airway inflammation by modulation pro-inflammatory cytokines through the matrix metallopeptidase 9…”