Effect of steroid and other hormones on lung removal of noradrenaline

Iwasawa, Yasuo; Gillis, Chris

doi:10.1016/0014-2999(73)90041-1

Cited by 15 publications

(10 citation statements)

References 10 publications

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“…Stereospecificity has also been demonstrated for the adrenergic neuronal uptake (Uptake,) of noradrenaline (Iversen, 1967) and for several amino acids by different tissues (Schultz & Curran, 1970). However, it was not possible to demonstrate stereospecificity for the uptake of noradrenaline (NA) by perfused rabbit lung (Iwasawa et al, 1973). The present data are not complete enough to draw any conclusions about the differential kinetic characteristics of the uptake of the two stereoisomers of methadone.…”

Section: Effect Oflack Ofglucosementioning

confidence: 54%

“…The results, therefore, would indicate that the site and the mechanism of (±)-methadone uptake may be different from those involved in the uptake of 5-HT and NA by the perfused lung. The results of Strum & Junod (1972) and that of Iwasawa et al (1973) have shown that the uptake of NA and 5-HT occurs mainly in the capillary endothelium. At present, it is difficult to speculate on the site of the uptake of (±)-methadone in the lung tissue.…”

Section: Effect Oflack Ofglucosementioning

confidence: 90%

“…(Alabaster & Bakhle, 1970;Rosenbloom & Bass, 1970;Junod, 1972a, b;Iwasawa, Gillis & Aghajanian, 1973;Orton, Anderson, Pickett, Eling & Fouts, 1973). The development of oedema has been the most common difficulty encountered in perfusion experiments using artificial media.…”

Section: Effect Oflack Ofglucosementioning

confidence: 99%

“…Iodoacetate is such a metabolic inhibitor that produces its effect through alkylation of -SH groups of enzymes, particularly those that are involved in the glycolytic pathway (Webb, 1963). Dixon (1937) (Takemori & Stenwick, 1966;Hug, 1967;Rubin, Owens & Rall, 1968;Iwasawa et al, 1973). One should, therefore, be careful in interpreting results obtained by using iodoacetate as a tool to evaluate the energy requirements or dependency of a transport process.…”

Section: Effect Oflack Ofglucosementioning

confidence: 99%

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Characterization of (±)‐methadone Uptake by Rat Lung

Chi

Dixit

1977

British J Pharmacology

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1By use of a sensitive and specific fluorescence assay procedure it was shown that after subcutaneous administration to rats, (±)rmethadone was concentrated in the lung. Lung to serum ratios ranging from 25 to 60 were obtained indicating that the rat lung tissue was capable of extracting (±)-methadone against a concentration gradient.2 This phenomenon was investigated in vitro with rat lung slices incubated in Krebs-Ringer phosphate buffer (pH 7.4). The uptake was expressed in terms of tissue to medium concentration ratios (T/M ratio).3 The principal observations were: (i) Studies on the time-course of the uptake showed that the T/M ratios of (±)-methadone increased rapidly during the first 60 min of incubation and then more slowly, with a plateau occurring at 180 min; (ii) The T/M ratio of (±)-methadone progressively increased from 9.5 to 17 as the pH of the incubation medium was varied from 6.2 to 7.8; (iii) When the concentration of (±)rmethadone in the incubation medium was varied from 0.005 to 0.5 mm, the T/M ratio decreased rapidly suggesting self-saturation of the transport process. Beyond the medium concentration of 0.5 mM, the T/M ratio declined very slowly. 4 These results suggested that at low concentrations, (±)-methadone was transported predominantly by a self-saturable process while at higher concentrations it was transported by a process of simple diffusion. 5 At low concentrations (0.01 mM) the uptake of (+)-methadone was higher than that of (-)-isomer indicating stereo-specificity of the uptake process. The uptake of (±)-methadone at low concentration (0.01 mM) was significantly inhibited by low temperature, lack of 02, lack of glucose, lack of Na+ in

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Section: Effect Oflack Ofglucosementioning

confidence: 54%

Section: Effect Oflack Ofglucosementioning

confidence: 90%

Section: Effect Oflack Ofglucosementioning

confidence: 99%

Section: Effect Oflack Ofglucosementioning

confidence: 99%

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Characterization of (±)‐methadone Uptake by Rat Lung

Chi

Dixit

1977

British J Pharmacology

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“…B6nisch, Uhlig & Trendelenburg, 1974) and in kitten isolated coronary arteries (Cornish & Goldie, 1980 (Iwasawa, Gillis & Aghajanian, 1973) and rat lung (Nicholas, Strum, Angelo & Junod, 1974) (Junod & Ody, 1977). It is most unlikely that noradrenergic nerves were involved in the accumulation of any of these catecholamines, since sympathetic innervation of airways smooth muscle is sparse or absent below the level of the bronchi in rat (Zussman, 1966;El-Bermani, 1978), guinea-pig (O'Donnell, Saar & Wood, 1978, rabbit and pig lung (Mann, (Hughes, Gillis & Bloom, 1969;Gillis, 1976) where cocaine and imipramine were potent uptake inhibitors (Iwasawa & Gillis, 1974;Nicholas et al, 1974) (Bakhle & Vane, 1974;Gillis & Roth, 1976;Sole et al, 1979) or in vitro (Gillis, 1976 (Bassett, 1971;Picken & Jarrott, 1975;Brine, Cornish & Miller, 1979). Cocaine (10 uM) and desipramine (1 pM), which did not significantly alter the cellular accumulation of radioactivity from [3H]-Iso, also failed to alter significantly the Iso concentrationeffect curves.…”

Section: Radio-tracer Studiesmentioning

confidence: 99%

Catecholamine Transport in Isolated Lung Parenchyma of Pig

Goldie

Paterson

1982

British J Pharmacology

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Normetanephrine (80upM) and 3-0-methylisoprenaline (50pM), which also depressed the accumulation of cellular radioactivitv from [3H]-Iso by > 50%, caused rightward shifts in Iso concentrationeffect curves as a result of P-adrenoceptor blockade. In sharp contrast, cortisol (80 AM) and U-0521 (60 MM), which caused smaller reductions in the cellular accumulation of radioactivity derived from [3H]-Iso, both caused an approximately 9 fold potentiation of responses to Iso in isolated lung strips. 5 The results indicate that the major sites of uptake andmetabolism of NA in porcine parenchyma strip are remote from a-adrenoceptors mediating NA-induced contraction. Similarly, some major sites of uptake of Iso are remote from P-adrenoceptors mediating Iso-induced relaxation. However, P-adrenoceptors are apparently in close proximity to a compartment containing COMT activity.

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5‐Hydroxytryptamine and Other Amines in the Lungs

Junod

1985

Comprehensive Physiology

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Effect of steroid and other hormones on lung removal of noradrenaline

Cited by 15 publications

References 10 publications

Characterization of (±)‐methadone Uptake by Rat Lung

Characterization of (±)‐methadone Uptake by Rat Lung

Catecholamine Transport in Isolated Lung Parenchyma of Pig

5‐Hydroxytryptamine and Other Amines in the Lungs

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