Effect of Stirring Duration on Hardness and Antibacterial Characteristics of Polyethylene Glycol-Hydroxyapatite Nanocomposites

Yudyanto, Yudyanto; Azizah, Erwina Nurul; Mufti, Nandang; Hartatiek,; Hidayat, Nurul; Kurniawan, Robi

doi:10.1088/1757-899x/515/1/012073

Cited by 5 publications

(1 citation statement)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mean Vickers microhardness of the green HA discs was 16 HV (0.16 GPa). These results are in agreement with that reported by Yudyanto et al 2019 who stated that the hardness of the prepared HA was 10 HV and the differences in the microhardness may be due to the different preparation methods [ 52 ]. Considering different HA sintering techniques, the linear increase of the Vickers microhardness with increasing sintering time was noted.…”

Section: Resultssupporting

confidence: 92%

Synthesized Nanorods Hydroxyapatite by Microwave-Assisted Technology for In Vitro Osteoporotic Bone Regeneration through Wnt/β-Catenin Pathway

et al. 2021

View full text Add to dashboard Cite

This research presents an optimal and inexpensive, without any additives, method for the synthesis and sintering of hydroxyapatite (HA) by microwave-assisted technology (MAT) furnace. The target sintering temperature of the furnace (1100 ℃) was held for one and two hours for conventional sintering. With regard to the microwave hybrid sintering, it was held at 100%MW for 20 and 30 min. FTIR, XRD, TGA, SEM/EDS, and TEM were assessed to determine HA phase composition, and structural as well as thermal decomposition behavior. The in vitro effects of sintered HA discs on cultured aged mice-isolated osteoblast cells and hydrocortisone-induced osteoclast cells were assessed by measuring ALP, osteocalcin, TRAP, calcium, and Alizarin red S staining. Moreover, their effects on cell differentiation (CD90 and CD 105 and PARR- ɣ) and death markers (GSK3b, MAPK, and β-catenin) were evaluated. The results demonstrate the production of ≈35 nm crystal-sized pure hydroxyapatite nanorod-like particles with a high degree of crystallinity and no impurities as required for biomedical application. HA increased osteogenesis (ALP, osteocalcin, and calcium) markers and decreased cell resorption markers. In addition, HA nanorods reversed the effect of cortisone on cell differentiation and death markers. In conclusion, microwave hybrid sintered HA is a potential nanomaterial for osteoporotic bone regeneration as HA reversed the cortisone adverse effect on osteoblast cell death through canonical and non-canonical pathways.

show abstract

Section: Resultssupporting

confidence: 92%