Effect of storage and heat on antimicrobial proteins in human milk.

Gibbs, Judith H.

doi:10.1136/adc.53.10.827-a

Cited by 1 publication

(1 citation statement)

References 5 publications

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“…We have recently reported that MOM and RM30 metabolomic profiles differ significantly from DHM (Torrez Lamberti et al, 2020). DHM is pasteurized because of safety concerns to reduce the presence of potential pathogens; however, the pasteurization process not only destroys the microbiota but also inactivates a great amount of valuable compounds as well (Evans et al, 1978;Landers and Updegrove, 2010;Montjaux-Régis et al, 2011;García-Lara et al, 2013;Marx et al, 2014). Therefore, personalization of DHM with FMOM could be a suitable strategy to provide enteral feeding to premature infants and provide all the intrinsic properties of MOM that may be lost if MOM is not stored at −20 • C.…”

Section: Discussionmentioning

confidence: 99%

Frozen Mother’s Own Milk Can Be Used Effectively to Personalize Donor Human Milk

et al. 2021

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Feeding preterm infants mother’s own milk (MOM) lowers rates of sepsis, decreases necrotizing enterocolitis, and shortens hospital stay. In the absence of freshly expressed MOM, frozen MOM (FMOM) is provided. When MOM is unavailable, preterm infants are often fed pasteurized donor human milk (DHM), rendering it devoid of beneficial bacteria. We have previously reported that when MOM is inoculated into DHM to restore the live microbiota [restored milk (RM)], a similar microbial diversity to MOM can be achieved. Yet, it is unknown if a similar diversity to MOM can be obtained when FMOM is inoculated into DHM. The goal of this study was to determine whether a similar microbial composition to MOM could be obtained when FMOM is used to personalize DHM. To this end, a fresh sample of MOM was obtained and divided into fresh and frozen fractions. MOM and FMOM were inoculated into DHM at different dilutions: MOM/FMOM 10% (RM/FRM10) and MOM/FMOM 30% (RM/FRM30) and incubated at 37°C. At different timepoints, culture-dependent and culture-independent techniques were performed. Similar microbiota expansion and alpha diversity were observed in MOM, RM10, and RM30 whether fresh or frozen milk was used as the inoculum. To evaluate if microbial expansion would result in an abnormal activation on the innate immune system, Caco-2 epithelial cells were exposed to RM/FRM to compare interleukin 8 levels with Caco-2 cells exposed to MOM or DHM. It was found that RM samples did not elicit a significant increase in IL-8 levels when compared to MOM or FMOM. These results suggest that FMOM can be used to inoculate DHM if fresh MOM is unavailable or limited in supply, allowing both fresh MOM and FMOM to be viable options in a microbial restoration strategy.

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