Effect of Storage Method on Spore Viability in Five Globally Threatened Fern Species

Quintanilla, Luís G.; Vázquez, Javier Amigó; Pangua, Emilia; Pajarón, Santiago

doi:10.1093/aob/mcf224

Cited by 49 publications

(41 citation statements)

References 23 publications

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“…Culcita macrocarpa C. Presl is the only European member of the family Dicksoniaceae with very large fronds, often over 2m long that are persistent in winter and arranged in crowns (Figure 1). The spores of this species are highly sensitive to desiccation (Quintanilla et al 2002). In northwest Spanish populations of C. macrocarpa sporangium dehiscence occurs around the spring equinox, when temperatures are suitable for germination (Quintanilla et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Effect of Nutrients on Environmental Sex Determination and Size of Gametophytes in Culcita macrocarpa

2014

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:In environmental sex determination (ESD) gender is decided after conception, depending on the environment, rather than being genetically fixed. ESD has rarely been studied in homosporous ferns. In the present study, Culcita macrocarpa gametophytes were cultured under varying nutrient conditions. Initially, most of the gametophytes of Culcita macrocarpa were male and subsequently hermaphrodite under different nutrition. The result indicates that its sex determination is protandry. All nutrient conditions were favourable for developing male prothalli but only good environment (high nutrient) was favourable for female gametophyte growth. In all respects, female gametophytes were much larger, than the other types of gametophytes. Hermaphroditic gametophytes were larger than male gametophytes, which were larger than asexual gametophytes.Key Words: Environmental sex determination, homosporous fern, Culcita macrocarpa, protandry, hermaphroditic. mvivsk t cwi ‡ekxq wj½ wba© vi ‡Yi(ESD) † ¶ ‡Î Mf© avi ‡Yi ci wj½ wba© viY RxbMZ wba© vi ‡Yi †P ‡q cwi ‡ekxq wba© vi ‡Yi Dci ‡ekx wbf© ikxj| mg ‡iYy cÖ m~ dv ‡Y© cwi ‡ekxq wj½ wba© viY m¤ú ‡K© wKQy M ‡elYv n ‡q ‡Q| eZ© gvb M ‡elYvq wewfboe cy wó ‡ ¶ ‡Î Culcita macrocarpa Gi Pvl Kiv n ‡q ‡Q| cÖ v_wgK ch© v ‡q wewfboe cy wóMZ Ae¯' vq C. macrocarpa Gi AwaKvsk wj ‡½i Dw™¢ cy i"l Ges cieZx© ‡Z Dfwj½| djvdj wb ‡`© k K ‡i †h,G ‡`i wj½ wba© viY †cÖ vUvwÛª (Protandry)| mKj cy wó gva¨g cy s †cÖ v ‡_jv ‡mi Rb¨ Dchy ³ wKš' ïay gvÎ fvj cwi ‡ekB ¯¿ x wj½a ‡ii Rb¨ Dchy ³| mKj † ¶ ‡Î, ¯¿ x wj½ai Ab¨ mKj wj½ai Dw™¢ ‡`i Zz jbvq AvKv ‡i A ‡bK eo| Dfwj½ wj½ai cy s-wj½ai †_ ‡K eo hv Avevi A ‡hŠb wj½ai †_ ‡K eo|

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Section: Introductionmentioning

confidence: 99%

Effect of Nutrients on Environmental Sex Determination and Size of Gametophytes in Culcita macrocarpa

2014

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“…This could represent an afterripening response in green spores but no previous reports of this phenomenon exist in the literature and this observation deserves further investigation. Increased post-harvest germination percentages of fern spores in comparison to those obtained for freshly harvested spores have been observed in spores stored in hydrated conditions (Quintanilla et al 2002) or after liquid nitrogen exposure (Filipin et al 2017). In these two situations the increased germination performance has been related to an artefact of faster germination of hydrated spores and incomplete germination time courses for freshly harvested spores (Quintanilla et al 2002) or to liquid nitrogen induced scarification that facilitated water imbibition and subsequent germination (Filipin et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Photo-oxidation modulates green fern spore longevity during dry storage

Ballesteros

Narayan

Varghese

et al. 2018

Plant Cell Tiss Organ Cult

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Desiccation tolerance and longevity of plant propagules in the dry state have significant implications for biotechnological applications. In this study fern spores were used as a unicellular model to characterize some of the mechanisms of ageing during dry storage of plant propagules (at relative humidity ca. 15%). More specifically, we compared the potential relationships among indicators of photo-oxidative stress and spore viability during dry storage between green (chlorophyllous) spores of Todea barbara and non-green spores of Christella dentata. Green spores stored under the light aged faster than those stored in the dark, and faster than light-and dark-stored non-green spores of C. dentata. This rapid ageing in light-stored green spores was associated with significantly lower antioxidant activity (relative to time zero and dark-stored spores) during storage, and a burst of hydrogen peroxide during the latter stages of storage, which was not a feature of dark-stored spores. We attribute these signs of enhanced oxidative-stress mediated ageing in light-stored spores to photo-oxidative processes, similar to those described in other homoiochlorophyllous organisms. Additionally, high antioxidant activity and low levels of reactive oxygen species in green spores compared with non-green spores suggests differing mechanisms of coping with life in the dry state.

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“…También su conservación en medio húmedo, como sería el suelo, ha sido estudiada en algunas especies de bosques higrófilos como Woodwardia radicans y Culcita macrocarpa y en tres especies de Dryopteris, D.corleyi, D. aemula y D. guanchica (Quintanilla et al 2002) que a 5 y 20 ºC conservaron sus porcentajes de viabilidad durante el año que duró el experimento. Lindsay et al (1992) comprobaron, en especies con esporas no clorofí-licas completamente hidratadas, entre las que se encontraba Athyrium filix-femina, que a temperatura ambiente (20 ºC) a lo largo de dos años conservaron la viabilidad inicial del 85%, mientras que en medio seco, el porcentaje descendió a un 17%.…”

Section: Discussionunclassified

El banco de esporas de "Athyrium filix-femina" y "Dryopteris filix-mas" en un pinar de la Sierra de Guadarrrama

et al. 2012

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INTRODUCCIÓNEl banco de semillas está considerado un elemento importante en la dinámica de poblaciones, si bien su comportamiento es muy variable dependiendo de las especies. En general, se considera que el banco de semillas tiene más importancia en plantas anuales, de hábitats más expuestos a perturbaciones más o menos catastróficas, que en especies perennes de bosques estables (Fenner & Thompson 2005). Estos aspectos más o menos conocidos en el caso de las plantas con semilla (Thompson et al. 1997), no se conocen en las plantas productoras de esporas, como son los helechos. Además, entre las plantas con semilla hay especies que hacen grandes aportaciones al banco de semillas, mientras que otras, por sus propios sistemas de dispersión, no pasan a formar parte de él. Sin embargo, la casi totalidad de los helechos producen cada temporada un elevado número de esporas, de las que supuestamente una buena parte pasan directamente a engrosar el banco del suelo, aunque también hay testimonios de que no todas las especies de helechos forman bancos de esporas (Ramírez-Trejo et al. 2004) Hay evidencias acerca de la importancia de los bancos de esporas de los helechos en el establecimiento y re- El banco de esporas se considera un elemento fundamental en la dinámica de las poblaciones de helechos. La confirmación de su existencia y el estudio de su comportamiento son, por tanto, básicos para conocer el funcionamiento de dichas poblaciones. Para confirmar la existencia y viabilidad del banco de esporas en un bosque de pino silvestre, en el que las especies dominantes de helechos son Athyrium filix-femina, y Dryopteris filix-mas, se tomaron muestras de suelo, y se cuantificó la cantidad de gametófitos de cada especie por muestra. Se obtuvieron muestras a diferentes profundidades, de 3-10, 10-20 y de 20-25cm, para comprobar la estructura vertical, y a 0, 2 y 5 metros de los esporófitos, para valorar el efecto de la distancia a la fuente de esporas. Se confirmó la existencia de un banco de esporas, y se observaron diferencias entre las dos especies siendo las esporas de Athyrium filix-femina las que dominan, con porcentajes de esporas viables mucho más elevados que los de Dryopteris con respecto a los factores estudiados. La mayor presencia de esporas de Athyrium en el banco podría ser la causa de la mayor abundancia de esta especie. The soil spore bank plays a pivotal role in fern population dynamics. Thus, the confirmation of its existence and the knowledge of its behaviour are basic to know population functioning. To study the presence and viability of a spore bank, soil samples were collected in a Scottish pine wood in which Athyrium filix-femina and Dryopteris filix-mas are the dominant fern species, and the amount of gametophytes of each species was quantified. Vertical structure, sampling at three different depths, from 3-10, 10-20 and 20-25 cm, and the effect of distance to spore source, sampling at 0, 2 and 5 m from sporophytes, were studied. We observed differences between both species, the spores of Athyrium filix-f...

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Effect of Storage Method on Spore Viability in Five Globally Threatened Fern Species

Cited by 49 publications

References 23 publications

Effect of Nutrients on Environmental Sex Determination and Size of Gametophytes in Culcita macrocarpa

Effect of Nutrients on Environmental Sex Determination and Size of Gametophytes in Culcita macrocarpa

Photo-oxidation modulates green fern spore longevity during dry storage

El banco de esporas de "Athyrium filix-femina" y "Dryopteris filix-mas" en un pinar de la Sierra de Guadarrrama

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