Effect of Strobilanthes crispus on Tumour Marker Enzymes and Glutathione During Chemical Hepatocarcinogenesis in the Rat

Suherman, Jo; Asmah, R.; Fauziah, O.; Patimah, Ismail; ., A. Nor Haslinda

doi:10.3923/pjbs.2004.947.951

Cited by 9 publications

(4 citation statements)

References 19 publications

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“…The water extract of this plant was reported to contain compounds with very high binding affinity to protein molecules, hence, inhibiting the proliferation of retroviruses [16]. Pharmacological studies have further shown the ability of S. crispus in preventing chemically-induced hepatocarcinogenesis in rats [17-19]. Administration of S. crispus extract also reduced the severity of hepatic necrosis in rats with diethylnitrosamine- and acetylaminofluorene-induced hepatocellular carcinoma and this was suggested to be due to the inhibition of enzymes involved in metabolic activation of the carcinogens [20].…”

Section: Introductionmentioning

confidence: 99%

Anticancer activity of a sub-fraction of dichloromethane extract of Strobilanthes crispus on human breast and prostate cancer cells in vitro

Yaacob

Hamzah

Kamal

et al. 2010

BMC Complement Altern Med

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BackgroundThe leaves of Strobilanthes crispus (S. crispus) which is native to the regions of Madagascar to the Malay Archipelago, are used in folk medicine for their antidiabetic, diuretic, anticancer and blood pressure lowering properties. Crude extracts of this plant have been found to be cytotoxic to human cancer cell lines and protective against chemically-induced hepatocarcinogenesis in rats. In this study, the cytotoxicity of various sub-fractions of dichloromethane extract isolated from the leaves of S. crispus was determined and the anticancer activity of one of the bioactive sub-fractions, SC/D-F9, was further analysed in breast and prostate cancer cell lines.MethodsThe dichloromethane extract of S. crispus was chromatographed on silica gel by flash column chromatography. The ability of the various sub-fractions obtained to induce cell death of MCF-7, MDA-MB-231, PC-3 and DU-145 cell lines was determined using the LDH assay. The dose-response effect and the EC50 values of the active sub-fraction, SC/D-F9, were determined. Apoptosis was detected using Annexin V antibody and propidium iodide staining and analysed by fluorescence microscopy and flow cytometry, while caspase 3/7 activity was detected using FLICA caspase inhibitor and analysed by fluorescence microscopy.ResultsSelected sub-fractions of the dichloromethane extract induced death of MCF-7, MDA-MB-231, PC-3 and DU-145 cells. The sub-fraction SC/D-F9, consistently killed breast and prostate cancer cell lines with low EC50 values but is non-cytotoxic to the normal breast epithelial cell line, MCF-10A. SC/D-F9 displayed relatively higher cytotoxicity compared to tamoxifen, paclitaxel, docetaxel and doxorubicin. Cell death induced by SC/D-F9 occurred via apoptosis with the involvement of caspase 3 and/or 7.ConclusionsA dichloromethane sub-fraction of S. crispus displayed potent anticancer activities in vitro that can be further exploited for the development of a potential therapeutic anticancer agent.

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Section: Introductionmentioning

confidence: 99%

Anticancer activity of a sub-fraction of dichloromethane extract of Strobilanthes crispus on human breast and prostate cancer cells in vitro

Yaacob

Hamzah

Kamal

et al. 2010

BMC Complement Altern Med

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“…More importantly, administration of SC extract did not physiologically and histologically affect the liver in normal rats in both studies. Hence, these studies demonstrated that S. crispus leaf extract, at the optimum dose of 5% (w/v), is able to alleviate the severity of liver cancer by lowering the activity of γ-glutamyl transpeptidase (GGT), alkaline phosphatase (ALP) and glutathione (GSH), glutathione s-transferase (GST), and uranyl diphosphate glucoronyl transferase (UDPGT) [79,80].…”

Section: In Vivo Studiesmentioning

confidence: 99%

“…Suherman et al also studied the effect of the aqueous extract of S. crispus leaves in the DEN/AAF-induced rats by examining the concentrations of plasma and liver enzymes, which are γ-glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), and glutathione (GSH) [79]. After determining the enzyme level, the results showed that in the DEN/AAFinduced cancer groups (C), there were significant increases (p < 0.05) in the levels of GGT, ALP, and GSH as compared to the normal control group (N).…”

Section: In Vivo Studiesmentioning

confidence: 99%

Anticancer Properties of Strobilanthes crispus: A Review

et al. 2021

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Cancer is a major cause of death worldwide, as exemplified by millions of cancer diagnoses every year. The use of chemotherapy in treating cancer has many disadvantages which include recurrence of cancer, associated with drug resistance, and severe side effects that are harmful to the patients. A better source of anticancer drugs can come from nature. Strobilanthes crispus (S. crispus) is a herbal medicinal plant that is indigenous in Madagascar and the Malay Archipelago. The plant possesses high vitamin and mineral content as well as phytochemicals—like phenols, catechins, tannins, and flavonoids—that are known to have therapeutic effects. Numerous preclinical studies have reported very versatile pharmacological effects of this plant, such as anticancer, antimicrobial, antioxidant, anti-angiogenesis, anti-diabetes, anti-ulcerogenic, and wound healing. Herein, this paper reviews the anticancer properties of S. crispus, providing information for future research and further exploration.

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“…The dichloromethane extract of S. crispus was reported to induce cell deaths in breast (MCF-7, MDA-MB-231) and prostate (PC-3, DU-145) cancer cell lines and was comparatively shown to be more potent than some anticancer drugs [1]. Meanwhile, in vivo studies concluded that S. crispus extract acted as chemopreventive agent with reduced chemically-induced hepatocarcinogenesis in rats [9], [10].…”

Section: Introductionmentioning

confidence: 99%

Strobilanthes crispus Extract Induces Apoptosis Through Enhanced Caspases Activities in Cervical Cancer Cells

2014

International Conference on Biological, Environment and Food Engineering (BEFE-2014) August 4-5, 2014 Bali (Indonesia)

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Strobilanthes crispus extract was found to exert cytotoxicity on several cancer cell lines and able to reduce chemically-induced hepatocarcinogenesis in rats. In this study, the cytotoxicity of S. crispus extracts that isolated from leaves and stem by hexane, chloroform, ethyl acetate, methanol and water solvent was determined and its possible underlying apoptotic mechanism was further investigated in HeLa cell line. MTT analysis demonstrated that only hexane-stem extract portrayed cytotoxic effects on HeLa cells with IC 50 of 160 μg/ml. Chloform extract showed possible cell inhibition trend while other extracts showed little or no cytotoxic effects. Hexane-stem extract was further analysed in this study where it was found to induce apoptosis, which was confirmed with obvious morphological changes in HeLa cells accompanied by sub-G 1 peak detection in cell cycle analysis by flow cytometry. Hexane-stem extract did not induce cell cycle arrest at any phases based on data shown. High level of caspase-3/7 activity detected indicated the involvement of caspase-3/7 activation in the apoptogenic effect induced by hexane-stem extract. Activity of caspase-8 and caspase-9 were found not significant in this study. More studies could be carried out to further elucidate the apoptotic mechanism clearly. Overall findings showed that S. crispus acted as apoptotic inducer which could be used as potential anticancer agent in the future.

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Effect of Strobilanthes crispus on Tumour Marker Enzymes and Glutathione During Chemical Hepatocarcinogenesis in the Rat

Cited by 9 publications

References 19 publications

Anticancer activity of a sub-fraction of dichloromethane extract of Strobilanthes crispus on human breast and prostate cancer cells in vitro

Anticancer activity of a sub-fraction of dichloromethane extract of Strobilanthes crispus on human breast and prostate cancer cells in vitro

Anticancer Properties of Strobilanthes crispus: A Review

Strobilanthes crispus Extract Induces Apoptosis Through Enhanced Caspases Activities in Cervical Cancer Cells

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