Effect of Subclinical Mastitis on Milk Profile Changes in Jersey Cross  Bred Dairy Cows

Balaji, N Sri; Sankar, V.; Senthilkumar, P.; Nalini, P; Muralidharan, J.; Senthilkumar, A.

doi:10.20546/ijcmas.2020.905.276

Cited by 2 publications

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“…In Egypt, subclinical mastitis is confirmed when SCC exceeds 500,000 cells/ml [30]. In Table 2, the average SCC of quarter milk samples and composite milk samples were similar to the average value (9.24 ± 2.0 × 105 cells/ml) reported by Skrzypek et al [48] and average (44.8 ± 0.26 x 104 cell/ml) reported by Mohamed et al [49], respectively. Coliforms are environmental mastitis-causing pathogens, they are primarily present in moisture, mud, faeces and other organic matter present around the animals.…”

Section: Discussionsupporting

confidence: 78%

Isolation and characterization ofE. colistrains causing intramammary infections from dairy animals and wild birds

Fahim

Ismael

Khalefa

et al. 2019

International Journal of Veterinary Science and Medicine

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The study was conducted to estimate the prevalence of Escherichia coli (E. coli) in sub-clinically mastitic (SCM) animals, and in wild and migratory birds which may act as reservoir disseminating such pathogen. Farm hygiene, management and milking procedures were listed through a questionnaire. Thirty lactating cows and 15 lactating buffaloes from five small-scale dairy farms were randomly selected and screened for subclinical mastitis (SCM) using California Mastitis Test (CMT) and somatic cell count (SCC). In addition, 80 teat skin swabs, 5 drinking water samples and 38 wild and migratory bird faecal matter were also collected. All samples were processed for E. coli isolation by culturing on Levine’s Eosin Methylene Blue (L-EMB) agar, followed by purification and biochemical identification. Positive samples were subjected to molecular identification and serotyping. In addition, the presence of extended-spectrum beta-lactamase (ESBL) and carbapenemase-producing E. coli have been reported by antimicrobial sensitivity testing. Escherichia coli were isolated from 7.7%, 50% and 50% of the positive CMT cows’ quarters, cows’ composite and buffaloes’ composite milk samples, respectively. In addition, 14% of cows’ teats, 20% of water samples, 70% of faecal matter from wild bird, and 33.3% of faecal matter from migratory waterfowls were carrying E. coli. Serotyping, antibiotic-resistant pattern and phylogenetic analysis have pointed the bearable implication of milking hygiene and wild birds in disseminating E. coli strains causing intramammary infections.

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Section: Discussionsupporting

confidence: 78%

Isolation and characterization ofE. colistrains causing intramammary infections from dairy animals and wild birds

Fahim

Ismael

Khalefa

et al. 2019

International Journal of Veterinary Science and Medicine

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“…Normal value of somatic cells in healthy udder ranged between 50,000 and 100,000 cells/ml of milk [26]. Sri Balaji et al [27] reported that the SCC, milk pH, and chloride contents in milk are increased in SCM affected milk samples as compared to that of healthy dairy cows. Average SCC from quarter milk samples measured by CMT score justified the BMT score Table 4.…”

Section: Resultsmentioning

confidence: 99%

Development and validation of BLRI Mastitis Test Kit at Bangladesh Livestock Research Institute Regional Station, Sirajganj

et al. 2019

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Objective:The objective of this study was to develop a low-cost kit for the detection of subclinical mastitis (SCM) and to check its validity, reproducibility, and efficacy at the field level.Materials and Methods:A total of 550 quarter milk samples from crossbred dairy cows were collected, of which 400 milk samples were used to validate the newly developed BLRI mastitis test (BMT) kit to justify its efficacy as an individual test kit in detecting SCM based on somatic cell count (SCC) by direct microscopic count (DMC). The efficacy of the newly developed BMT was compared with the California Mastitis Test (CMT) kit. Another 150 milk samples were subjected to SCC determined by DMC and DCC (De Laval cell counter®) categorized by CMT and BMT scores.Results:A SCM test kit, namely, BMT kit was successfully developed in this study. The percentage accuracy of CMT and BMT were 76.75% and 75.75%; sensitivity 69.36% and 67.56%; specificity 85.95% and 85.85%; positive predictive value 86.03% and 85.71%; negative predictive value 69.23% and 68%, respectively. A p value of 0.001 was found for both CMT and BMT. However, CMT and BMT had no significant difference in sensitivity (p = 0.778). Average SCCs (cells/ml) determined by DCC and DMC, respectively, were mostly corresponded to the SCC ranges of both CMT and BMT scores.Conclusion:The newly developed BMT kit is an independent, cheap, farmer-friendly, first country made, and reliable SCM diagnostic test kit that can be used at field condition.

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Effect of Subclinical Mastitis on Milk Profile Changes in Jersey Cross Bred Dairy Cows

Cited by 2 publications

References 0 publications

Isolation and characterization ofE. colistrains causing intramammary infections from dairy animals and wild birds

Isolation and characterization ofE. colistrains causing intramammary infections from dairy animals and wild birds

Development and validation of BLRI Mastitis Test Kit at Bangladesh Livestock Research Institute Regional Station, Sirajganj

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