Effect of sucrose application, minerals, and irradiance on the in vitro growth of Cistus incanus seedlings and plantlets

Abstract

To study the effect of sucrose on the sink-source relationship in in vitro-grown plants, Cistus incanus seedlings and plantlets were grown horizontally in a two-compartment Petri dish (split dish), with the root system in one compartment and the shoot in the other. Shoots and roots were exposed to different sucrose concentrations (0 -30 g dm -3 ), two irradiance levels (25 and 160 µmol m -2 s -1 ) and the presence or absence of a minimum medium containing minerals and vitamins (M medium). Root and shoot biomass of the seedlings was enhanced by an increase in irradiance when the growth medium was not supplemented with sucrose indicating the role of photosynthesis in biomass production. When sucrose was added to either organ growth was enhanced as well. In the presence of sucrose in the root compartment, sucrose applied to the shoot compartment enhanced growth of both organs under low irradiance, while under high irradiance, sucrose had no further additive effect. In the absence of sucrose in the root compartment, the enhancement of root biomass by sucrose added to the shoot compartment was lower under high irradiance than under low irradiance. The response of Cistus plantlets to sucrose and irradiance differed from that of seedlings, probably reflecting a greater susceptibility of the plantlets to sucrose feedback inhibition on photosynthesis and biomass accumulation. The decrease in root and shoot growth when M medium was added to the shoot compartment and the relatively better growth of these organs when the roots were supplied with minerals and the shoot with sucrose, indicate that growth of the two organs in our experimental set-up was regulated by opposing fluxes of C and nutrients.Additional key words: sink-source relationship, two-compartment Petri dish.

Chromosomal localization of selected SCARs converted from new RAPD, ISSR and R-ISSR markers linked to rye (Secale cereale L.) tolerance to nutrient deprivation stress identified using bulk segregant analysis

“…The authors suggest that the characteristics may modify the phenotypic response of examined genotype depending on the composition of used medium, which makes some components of the medium unavailable to plants. Other factors affecting seedling growth in a mature embryo culture may include embryo length (Monnier, 1990) and C:N ratio (Malamy and Ryan, 2001;Mills, 2009). …”

Improved growth and acclimatization of somatic embryo-derived Oplopanax elatus plantlets by ventilated photoautotrophic culture

“…The poor acclimatization of somatic embryoderived plantlets is, however, a serious handicap (Moon et al 2006). Recently, a photoautotrophic propagation methodology was applied to improve the physiological, anatomical, and morphological characteristics of in vitro grown plantlets (Mills 2009). Plants grown by this method showed better acclimatization to ex vitro transplantation (Kozai 1991a,b, Ziv 1995, Lian et al 2002, Zobayed et al 2004, Nguyen and Kozai 2005.…”

“…Culture conditions sustaining high multiplication rates have a major effect on the growth and physiology of developing plantlets (Ziv 1995). Leaves are the major organ affected during plantlet growth in vitro (Mills 2009); although the stem is also affected, it has (unless very malformed) a smaller immediate impact on plant water stress and ex vitro survival. In this study, the plantlets, grown under the photoautotrophic condition with forced ventilation, possessed more leaves (2.8 per plant) and larger leaf area (18.9 cm 2 ) than those cultured under the conventional and the photoautotrophic (without ventilation) conditions (Table 1, Fig.…”

Evaluation of the effect of in vitro stress and competition on tissue culture response of flax

“…At distances above 1.0 cm, plant development was getting lower again. Mills (2009) has stated that sucrose in the growth medium improves biomass production and photosynthetic activity by increasing leaf area. Mingozzi and Morini (2009) have reported that physical microenvironment affects in vitro regeneration significantly.…”