Effect of Sulphur Deficiency on Fermentation of Different Carbohydrate Sources in an Artificial Rumen (Rusitec)

Stevani, J.; Durand, Michelle

doi:10.5713/ajas.1989.388

Cited by 2 publications

(2 citation statements)

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“…When a large amount of urea to substitute natural protein in a ruminant diet, limited sulfur intake and may restrict microbial protein synthesis (Pathak, 2008). The sulfur capture for synthesis of microbial methionine and cystine requires more degradable source of feed carbohydrate (Stevani and Durand, 1989). However, it is not clear why all in situ degradation characteristics of sulfur decreased (P<0.05) with increasing the synchronization index in TMR (Table 3).…”

Section: In Situ Sulfur Degradability Of Tmrmentioning

confidence: 98%

“…Sulfur intake restriction may lower the synthesis of methionine by bacteria, thus microbial protein synthesis may be adjusted to the availability of ruminal sulfur (Karto, 1999). Likewise, dietary sulfur concentration has been found to effect on rumen microbial growth (Suttle, 2010;Pathak, 2008;Stevani and Durand, 1989). The synchronization of supply from ruminal product of carbohydrate and nitrogen degradations is expected to increase microbial protein synthesis.…”

Section: Introductionmentioning

confidence: 99%

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Synchronization of Carbohydrate and Protein Supply in the Sugarcane Bagasse Based Ration on in Situ Nutrient Degradability

Sani

Nuswantara

Pangestu

et al. 2016

J. Indonesian Trop. Anim. Agric.

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Two adult male sheeps fitted with rumen cannula were used in two experiments to study the effects of synchronization of carbohydrate and nitrogen supply in sugarcane bagasse based total mixed ration (TMR) on in situ nutrient degradation. The first experiment was aimed to create three TMR with different synchronization index. Ingredient feedstuffs of TMR were evaluated for its in situ organic matter (OM) and nitrogen (N) degradation kinetics. On the basis of the OM and N degradation kinetics of feedstuffs, three sugarcane bagasse based TMR were formulated with synchronization indexes of 0.37; 0.50; and 0.63; respectively. The TMR had similar levels of crude protein (CP), total digestible nutrients, and neutral detergent fiber (NDF). In the second experiment, the three TMR with different synchronization index were evaluated for in situ degradability characteristics of OM, CP, NDF, and sulfur. The in situ degradation of OM in TMR were decreased (P<0.05) with the increasing of synchronization index. The higher synchronization index in TMR increased (P<0.05) CP degradation of CP. The NDF degradation decreased slightly by the alteration of synchronization index in TMR. The higher synchronization index in TMR reduced (P<0.05) in situ sulfur degradation, and this may not support to effects of synchronization of carbohydrate and nitrogen supply.

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Section: In Situ Sulfur Degradability Of Tmrmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Synchronization of Carbohydrate and Protein Supply in the Sugarcane Bagasse Based Ration on in Situ Nutrient Degradability

Sani

Nuswantara

Pangestu

et al. 2016

J. Indonesian Trop. Anim. Agric.

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Influencia del azufre y del tipo de sustrato sobre el crecimiento de los hongos ruminales Neocallimastix frontalis y Orpinomyces intercalaris

Jiménez

Benítez

2005

CTA

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Se evaluó la colonización in vitro y la actividad enzimática extracelular (xilanolítica y celulolítica) de los hongos ruminales Neocallimastix frontalis H10001 y Orpinomyces intercalaris H20013 sobre hojas de las gramíneas Brachiaria decumbens y Pennisetum clandestinum. Para promover la colonización y actividad enzimática se utilizaron tres niveles de sulfato de sodio (0%, 4% y 12% p/v). La colonización se evaluó determinando el número de esporangios·mm–2 y la actividad enzimática se evaluó en el sobrenadante a las 72 h por cuantificación de los azúcares reductores liberados durante el proceso. El número de esporangios varió (P < 0,05) con la interacción tipo de gramínea × nivel de azufre. Para N. frontalis la adición de S incrementó el número de esporangios excepto para N. frontalis sobre B. decumbens (P < 0,05); mientras que para O. intercalaris la adición de S incrementó el número de esporangios en ambas gramíneas (P < 0,05). El mayor número de esporangios se obtuvo con O. intercalaris sobre P. clandestinum en las concentraciones de 4 y 12% de S (12,77 y 14,82 esporangios·mm–2, respectivamente). La actividad xilanolítica extracelular varío (P < 0,05) con el tipo de gramínea; la mayor actividad xilanolítica extracelular se determinó en el sobrenadante de O. intercalaris sobre B. decumbens. La actividad celulolítica extracelular varió (P < 0,05) con la interacción tipo de gramínea × nivel de azufre. La utilización de 4% de S incrementó (P < 0,05) la actividad celulolítica extracelular de los sobrenadantes de N. frontalis y O. intercalaris en ambas gramíneas; mientras que la adición de 12% de S no afectó (P > 0,05) la actividad celulolítica de N. frontalis sobre P. clandestinum y de O. intercalaris sobre B. decumbens en comparación con el control; no obstante, la actividad celulolítica extracelular en los sobrenadantes de N. frontalis sobre B. decumbens y de O. intercalaris sobre P. clandestinum se redujeron (P < 0,05). Los resultados de este estudio indican que el número de esporangios y la actividad celulolítica extracelular de cultivos puros de los hongos N. frontalis y O. intercalaris dependen del tipo de gramínea y del nivel de S presente en el medio de cultivo; así mismo, la presencia de S mejora el crecimiento y desarrollo de los hongos. Influence of sulphurum and type of substrate on the growth of ruminal fungi Neocallimastix frontalis and Orpinomyces intercalarisThe study was aimed at evaluating in vitro fungal colonization and specific enzyme acti-vity (xylanase and cellulase) of Neocallimastix frontalis H10001 and Orpinomyces intercalaris H20013 rumen fungi on Brachiaria decumbens and Pennisetum clandestinum leaves. Three sodium sulphate levels (0%, 4% and 12% w/v) were used for testing in vitro fungal colonization and enzyme activity. Colonization was evaluated by quantifying the number of sporangia on leaf fragments. Enzyme activity was determined by measuring the amount of reduced sugar on supernatant fraction. Type × grass and sulphur interaction levels (P < 0.05) affect sporangial number. The sporangial number for N. frontalis increased (P < 0.05) when S was added, except for N. frontalis on B. decumbens, whilst adding S increased (P < 0.05) O. intercalaris sporangial number in both grasses. The highest sporangial numbers were detected on O. intercalaris and P. clandestinum at 4% and 12% S (12.77 and 14.82 sporangia·mm–2, respectively). Extracellular xylanolytic activity was affected (P < 0.05) by grass type. The highest extracellular xylanolytic activity was detected in O. intercalaris supernatant on B. decumbens. Extracellular cellulolytic activity was affected (P < 0.05) by type × grass sulphur interaction levels. Adding 4% S increased (P < 0.05) extracellular cellulolytic activity in N. frontalis and O. intercalaris supernatants in both grasses; there were no effects on extracellular cellulolytic activity from N. frontalis supernatant on P. clandestinum and O. intercalaris supernatant on B. decumbens compared to control. Extracellular cellulolytic activity was reduced (P < 0.05) in N. frontalis on B. decumbens and O. intercalaris on P. clandestinum. Variation in sporangial number and of pure rumen fungi culture (N. frontalis and O. intercalaris) extracellular cellulolytic acti vity depended on grass type as well as culture medium S level. S could improve rumen fungi growth and development.

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Effect of Sulphur Deficiency on Fermentation of Different Carbohydrate Sources in an Artificial Rumen (Rusitec)

Cited by 2 publications

References 0 publications

Synchronization of Carbohydrate and Protein Supply in the Sugarcane Bagasse Based Ration on in Situ Nutrient Degradability

Synchronization of Carbohydrate and Protein Supply in the Sugarcane Bagasse Based Ration on in Situ Nutrient Degradability

Influencia del azufre y del tipo de sustrato sobre el crecimiento de los hongos ruminales Neocallimastix frontalis y Orpinomyces intercalaris

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