Effect of Supplemental Oregano Essential Oils in Diets on Production Performance and Relatively Intestinal Parameters of Laying Hens

Abstract

This study was designed to investigate the effects of dietary oregano essential oils on 150 30-week-old Hy-Line Layers' productive performance, egg quality characteristics, cecal microbiota, digestive enzyme activities, mucosa structure of the duodenum and jejunum and glucose and small peptides transporters expression in the duodenum and jejunum. All hens were allocated into one of five different groups: negative control (NC; basal diet only), antibiotics control (CS; basal diet plus 100 mg/kg of 10% colistin sulfate) and treatments I, II, and III (basal diet supplemented with 50, 100 and 150 mg/kg oregano essential oils, respectively). The results showed that the laying rate, average egg weight, feed conversion ratio and the activities of amylase and trypsin were significantly improved by a diet supplemented with 100 mg/kg oregano essential oils (P < 0.01). The addition of oregano essential oils increased the number of intestinal Bifidobacterium and Lactobacillus significantly (P < 0.01), whereas the number of intestinal Escherichia coli and Salmonella was significantly (P < 0.01) decreased. The addition of 100 mg/kg oregano essential oils increased duodenum villus height (P > 0.05), significantly increased duodenum villus-height-tocrypt-depth ratios (P < 0.01) and decreased crypt depth in the duodenum (P < 0.05). Furthermore, the glucose transporter 2 (GLUT2), peptide transporter 1 (PepT1) and sodium-glucose cotransporter 1 (SGLT1) gene expression levels in the duodenum and jejunum were significantly increased in laying hens on a diet supplemented with 100 mg/kg oregano essential oils (P < 0.01). However, egg weight, relative eggshell weight, yolk index and Haugh unit value were not significantly affected by the addition of oregano essential oils (P > 0.05).

Dietary supplementation of plant essential oil improves growth performance, intestinal morphology and health in weaned pigs

“…The expression level of GLUT2 was also up‐regulated in the jejunum and ileum mucosa. The results obtained in this study were consistent with previous report that both the SGLT1 and GLUT2 genes in the intestinal mucosa were significantly up‐regulated by oregano essential oils in laying hens (He et al, ). These results provided a molecular basis for improved nutrient absorption after dietary PEO supplementation.…”

Influence of Using Dietary Antioxidant on Productive and Reproductive Performance of Mandara Strains Under Summer Conditions

“…The same later author reported that the use of 1.0% oregano in the diet was increased egg production and egg weight of hens. He et al (2017) indicated that oregano essential oil enhanced average egg weight and feed efficiency on laying hens. These results the oregano essential oils may enhance the intestinal health and digestibility of the feeds and thus improve the performance of laying hens (He et al, 2017).…”

“…These results are in agreement with those reported by Oussalah et al (2006), who studied 3 oregano varieties: the first (Origanum heracleoticum) from France, rich in carvacrol (54%); the second (Origanum compactum) from Morocco, rich both in carvacrol (22%) and γ-terpinene (23%); and the third (Origanum majorana) from Egypt, rich in terpinene-4-ol (26%) and γterpinene (12%). Mathlouthi et al (2012) and He et al (2017) found that the essential oil isolated from oregano was characterized by its increased content of carvacrol (69.55%) and (73.06), respectively.…”

“…Real segments of oregano are carvacrol and thymol that constitute about 78 to 82% of the total oil (Lambert et al, 2001). He et al (2017) showed that the laying rate, egg weight, feed conversion ratio and the activities of amylase and trypsin of Hy-Line layers were significantly (P < 0.01) improved by a diet supplemented with 100 mg/kg oregano essential oils. Roofchaee et al (2011) demonstrated that 600 mg/kg of oregano essential oil applied growth promoting impacts and furthermore showed strong antibacterial effects against cecal E. coli in grower diets of broiler.…”

Alternatives to the Use of Antibiotics in Egg Production

“…In addition to the pharmacologically active principle, the essential oils consist of mixtures of volatile substances, usually lipophilic, whose components include terpene hydrocarbons, simple alcohols, aldehydes, ketones, phenols, esters, organic acids, etc [3] A research [4] evaluating layers fed with colistin rations (100mg/ kg), and doses of oregano essential oil (50, 100 and 150mg / kg of ration), observed a better egg laying rate and egg weight with 100mg/kg of oregano essential oil. Likewise, this treatment promoted a better mass conversion of eggs, with no losses in egg quality, considering Haugh units.…”