Effect of surface roughness on the initial response of MC3T3-E1 cells cultured on polished titanium alloy

Abstract

Abstract. Surface roughness has been considered as an important influencing factor for cell response. The aim of this study was to find out whether MC3T3-E1 cells, a mouse osteoblast-like cell line, can sense the amplitudes of surface topography of titanium alloy (Ti6Al4V), and if surface-dependent cell morphology would be presented on the substrata with varied roughness. A series of polished samples (R a : 0.30~1.80 µm) were prepared to produce macroscopically parallel grooves using different grades of silicon carbide sandpaper (#100, #320, #600, #1000 and #2000). The experimental results indicated that the behavior and morphology of cells largely depended on the substrata where they were cultured. More efficient proliferation of MC3T3-E1 cells was shown on the surfaces with R a of 0.50~1.00 µm, with respect to either the rougher or the smoother specimens. Furthermore, MC3T3-E1 cells seeded on the Ti6Al4V surfaces within this narrow range responded to the increasing surface roughness with increased proliferation. Contact guidance of cells could be observed on the rougher specimens (R a : 0.80~1.00 µm), whereas more random orientations were exhibited for the adsorbed cells on the smoother surfaces (R a : 0.50~0.60 µm).

Antibacterial activity and biofilm inhibition by surface modified titanium alloy medical implants following application of silver, titanium dioxide and hydroxyapatite nanocoatings

“…The uncoated Ti6Al4V control discs were smooth with an Ra value of 0.358 ± 0.011 lm, and in agreement with previous reports for Grade 5 titanium alloy implants (0.30-0.50 lm, Wu et al, 2015). Although the chemistry and the nanotopography of the disc surfaces changed as a result of the anodisation and silver plating processes (Figure 1), the surface roughness values were no different to the uncoated controls (Table 1).…”

Low‐pressure plasma surface modification of polyurethane films with chitosan and collagen biomolecules

“…It has been proven that cellular behavior, such as spreading, adhesion, diffusion, and proliferation, is strongly influenced by some effective factors including roughness, hydrophilicity, morphology, and chemistry of the biomaterial surface. Surface wettability has been reported to have a significant effect on primary cell adhesion . Figure shows that the cell growth on the films increased from 12 to 72 h of incubation.…”

Exploring microfluidics as a tool to evaluate the biological properties of a titanium alloy under dynamic conditions

“…Noteworthy, previously it has been shown that osteoblast morphology and alignment can also be affected by topographical characteristics of the substrate, particularly when these features exhibit similar dimensions as the cellular microenvironment. 47,48 However, since no differences in cell morphology were observed between cells grown on Ti-static and PS-static for 24 hours (Fig. 6A), the changes in cell morphology and alignment were likely affected by the fluid flow, rather than due to contact guidance by the biomaterial surface.…”