Effect of susceptibility to enterotoxigenic Escherichia coli F4 and of dietary tryptophan on gut microbiota diversity observed in healthy young pigs

Messori, Stefano; Trevisi, Paolo; Simongiovanni, A.; Priori, Davide; Bosi, Paolo

doi:10.1016/j.vetmic.2012.09.001

Cited by 36 publications

(34 citation statements)

References 28 publications

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“…This hypothesis is not completely supported by our results on gut microbiota composition since we did not identify any effect of MUC4 genotype for that. Our result partially contrast with the previous study by Messori et al () in which a higher presence of Clostridium bartlettii was found for MUC4 GG and MUC4 CG than MUC4 CC pigs; however, it is generally recognized that the characterization by the sequencing of defined regions of 16S rRNA gene could have some limitations, such as an incomplete resolution and a low sensitivity (Poretsky, Rodriguez‐R, Luo, Tsementzi, & Konstantinidis, ); thus, we cannot conclude that gut microbiota is not influenced by MUC4 genotype and further investigations are required.…”

Section: Discussioncontrasting

confidence: 99%

“…This hypothesis is not completely supported by our results on gut microbiota composition since we did not identify any effect of MUC4 genotype for that. Our result partially contrast with the previous study by Messori et al (2013) (Gevers et al, 2015). Little information is reported on the FUT1 effect on intestinal microbiota.…”

Section: Discussioncontrasting

confidence: 99%

“…Studies suggested that MUC4 and FUT1 genotypes are not only associated with piglets susceptibility to PWD but can also influence the intestinal homoeostasis. For instance, healthy piglets with different MUC4 and FUT1 genotypes are known to differ for their intestinal microbial profile ( MUC4 , Messori, Trevisi, Simongiovanni, Priori, & Bosi, ; FUT1 , Poulsen et al, ) and for their intestinal protein glycosylation (Hesselager, Everest‐Dass, Thaysen‐Andersen, Bendixen, & Packer, ). Furthermore, the intestinal mucosa of piglets with the susceptible genotype ( MUC4 CG/GG ) had an up‐regulation of genes related to antimicrobial peptide and immune function such as lipopolysaccharide binding protein ( LBPI ) and regenerating islet‐derived 3 gamma ( REG3G) compared to pigs with a resistant genotype (Trevisi et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Mucine 4 and Fucosyltransferase 1 genetic variants on gut homoeostasis of growing healthy pigs

Luise

Motta

Bertocchi

et al. 2019

Animal Physiology Nutrition

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Putative genetic markers have been associated with ETEC F4 (Mucine 4 [MUC4]; MUC4GG;CG as susceptible; MUC4CC as resistant) and F18 (Fucosyltransferase 1 [FUT1]; FUT1GG;AG as susceptible; FUT1AA as resistant) resistances respectively. In this study, 71 post‐weaning pigs were followed from d0 (35 days old) to d42 (77 days of age) to investigate the effect of MUC4 or FUT1 genotypes on the mid‐jejunal microbiota composition, pigs expression of genes related to inflammation (IL8, GPX2, REG3G, TFF3, CCL20 and LBPI) and glycomic binding pattern profile (Ulex europaeus agglutinin I [UEA] fucose‐binding lectin and peanut agglutinin [PNA] galactose‐specific), and on blood plasma targeted metabolomics profile, faecal score and performance parameters of growing healthy pigs. The MUC4 and FUT1 resistant genotypes improved the pigs’ growth performance and had firmed faecal score susceptible genotypes in d0–d21 period. Pigs with MUC4GG genotype had a higher jejunal expression of genes relate to immune function (CCL20 and REG3G) than MUC4CG and MUC4CC pigs (p < 0.05). MUC4CG pigs had higher expression of TFF3 (implicated in mucosal integrity) than MUC4GG and MUC4CC (p < 0.05). FUT1 influenced the alpha‐ and beta‐jejunal microbial indices. The FUT1AA group had a higher number of operational taxonomic units (OTUs) belonging to Lactobacillus genus, while FUT1GG group had a higher number of OTUs belonging to Veillonella genus. MUC4CC pigs had lower scores for UEA on brush borders and goblet cells in villi than MUC4GG (p < 0.05). FUT1AA pigs had lower UEA positivity and higher PNA positivity on brush borders and goblet cells than FUT1AG and FUT1GG (p < 0.05). Both FUT1 and MUC4 influenced the metabolic profile of healthy pigs. Results highlight the role of MUC4 and FUT1 on pig intestinal homoeostasis and improved the knowledge regarding the potential interaction between host genetics, gut microbiota composition and host metabolism in a healthy status.

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Section: Discussioncontrasting

confidence: 99%

“…This hypothesis is not completely supported by our results on gut microbiota composition since we did not identify any effect of MUC4 genotype for that. Our result partially contrast with the previous study by Messori et al (2013) (Gevers et al, 2015). Little information is reported on the FUT1 effect on intestinal microbiota.…”

Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Mucine 4 and Fucosyltransferase 1 genetic variants on gut homoeostasis of growing healthy pigs

Luise

Motta

Bertocchi

et al. 2019

Animal Physiology Nutrition

Self Cite

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show abstract

“…Among these, of particular epidemiological importance for pigs are bacteria of the genera Escherichia (20), Salmonella (12), Enterobacter (30), Proteus (8), Citrobacter (9,23) and Providencia (7,32). Strains belonging to these genera were isolated from the cecum and colon of the four experimental groups whose diet was supplemented with a pre-and/or probiotic.…”

Section: Resultsmentioning

confidence: 99%

Effect of inulin and a probiotic supplement in the diet of pigs on selected traits of the gastrointestinal microbiome

Grela¹,

Kowalczyk-Pecka²,

Hańczakowska³

et al. 2016

Medycyna Weterynaryjna

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SummaryThe addition of inulin and/or a probiotic to feed mixtures for pigs can lead to changes in microflora composition and production of short-chain organic acids (SCFA) in the final segment of the digestive tract. The aim of the study was to determine the effect of an inulin and/or a probiotic supplement on Enterobacteriaceae taxa, the susceptibility of the bacteria to six antibiotics, and SCFA content in the cecum and colon, as well as changes in the histological structure of the intestines. The experiment was conducted on 80 fattening pigs with an initial body weight of 30.0 ± 0.5 kg, divided into 4 groups: control (I-C) and three experimental ones, fed the diets either with a probiotic (group II-P), inulin (III-I) or a both additives (group IV-PI). The animals were given ad libitum access to grower (30-70 kg) and finisher (71-115 kg) feed mixtures. During slaughter, from 8 pigs of each group, samples of contents and tissue from the cecum and colon were collected for laboratory analysis. The results of the study indicate differences in the expression of antibiotic resistance in the Enterobacteriaceae isolates depending on the type of dietary supplement. The inulin supplement caused changes in SCFA concentration, mainly an increase in the concentration of propionic and butyric acid, and also increased the thickness of the muscular layer.The synbiotic (mixture of prebiotics and probiotics) was confirmed to have a beneficial effect on the gastrointestinal microbiome, which may be of significance in preventing the spread of Enterobacteriaceae infections in pigs.

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“…Via portal vein, the intestine absorbed MC-LR as well as metabolic products of gut microflora could be delivered from gut to liver and other organs by blood (Faber et al, 2003;Goel et al, 2014). Gastroin-testinal ailments induced by MCs have been reported in fish, mice and humans (Humpage et al, 2000;Botha et al, 2004;Messori et al, 2013). For instance, MC-LR can induce cell apoptosis in the intestinal tract in mice (Botha et al, 2004), and inhibit activities of the digestive enzymes from intestinal mucosa and cause intestinal lesions (Liu and Xie, 2014).…”

Section: Introductionmentioning

confidence: 99%

Effects of microcystin-LR on gut microflora in different gut regions of mice

et al. 2015

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-To reveal the toxicological effects of the hepatotoxic microcystin-leucine arginine (MC-LR) on gut microbial community composition in different gut regions, we conducted a subchronic exposure of BALB/c mice to MC-LR via intragastric administration. Denaturing gradient gel electrophoresis (DGGE) was employed to profile the shifts of microbes after MC-LR treatment in the jejuno-ileum, caecum and colon. DGGE profiles analysis showed that MC-LR increased the microbial species richness (number of microbial bands) in the caecum and colon as well as microbial diversity (ShannonWiener index) in the caecum. The cluster analysis of DGGE profiles indicated that the microbial structures in the caecum and colon shifted significantly after MC-LR treatment, while that in the jejuno-ileum did not. All the relatively decreased gut microbes belonged to Clostridia in the Firmicutes phylum, and most of them were Lachnospiraceae. The increased ones derived from a variety of microbes including species from Porphyromonadaceae and Prevotellaceae in the Bacteroidetes phylum, as well as Lachnospiraceae and Ruminococcaceae in the Firmicutes phylum, and among which, the increase of Barnesiella in Porphyromonadaceae was most remarkable. In conclusion, subchronic exposure to MC-LR could disturb the balance of gut microbes in mice, and its toxicological effects varied between the jejuno-ileum and the other two gut regions.

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Effect of susceptibility to enterotoxigenic Escherichia coli F4 and of dietary tryptophan on gut microbiota diversity observed in healthy young pigs

Cited by 36 publications

References 28 publications

Effect of Mucine 4 and Fucosyltransferase 1 genetic variants on gut homoeostasis of growing healthy pigs

Effect of Mucine 4 and Fucosyltransferase 1 genetic variants on gut homoeostasis of growing healthy pigs

Effect of inulin and a probiotic supplement in the diet of pigs on selected traits of the gastrointestinal microbiome

Effects of microcystin-LR on gut microflora in different gut regions of mice

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