Effect of Swim-Up and Glass Wool Techniques, With Adding Antioxidants to Tris Extender on Improving Post-Cryopreserved Some Semen Attributes of Low Semen Quality for Holstein Bulls

Abstract

This study was conducted to investigate the effect of swim-up and glass wool as sperm separation techniques with adding vitamin E and superoxide dismutase (SOD) to Tris extender on improving some post-cryopreserved semen quality for Holstein bulls. Low and good of semen were extended using Tris extender. Good semen quality (GSQ) was divided into 3 groups (L1; Tris extender, L2; 2 mM vitamin E, L3; 200 IU SOD) Low semen quality (LSQ) was divided into two main groups, and subdivided into 3 sub-groups (L4; Tris extender, L5; 2 mM vitamin E, L6; 200 IU SOD). In the second main group, swim-up and glass wool techniques were used with adding vitamin E and SOD and subdivided into 3 sub-groups with each technique, and referred to L7, L8 and L9 for swim-up technique and L10, L11 and L12 for glass wool technique. Improving sperms’ cell motility and live sperm and reducing total sperms’ abnormalities percentages of low semen quality were noticed using swim-up and glass wool techniques were used with adding vitamin E and SOD. In conclusion, glass wool filtration separates out dead, abnormal and immotile sperm cells, a good sperm harvested by this technique.

Effect of Glass Wool and Sephadex Sperm Separation Techniques on Improving the Poor Quality Semen of Iraqi Buffalo Bulls

“…This result differed from the study conducted by [37], who noticed that head abnormalities of crossbred bulls were lower when using the glass wool (1.97 ± 0.64%) separation technique in comparison with the control treatment (4.60 ± 1.11%). Hassan [23] showed a decline in head abnormalities in Holstein bulls with the glass wool (0.70±0.29%) technique in comparison with a control group (low semen quality; 4.15±0.63%). While these results were in agreement with the findings of Vyas [37], Ahmad [14], and Maurya [33], which found a decline in head sperm abnormalities in crossbred bull and buffalo bull semen post-filtration using the Sephadex technique compared to the control group.…”

“…The reduction in the concentration of spermatozoa in groups following in vitro separation procedure may be due to the inability of the abnormal and dead sperms with poor motility to pass through glass wool and Sephadex filtration [31]. Removal of such detrimental sperm population from the ejaculates through glass wool or Sephadex filtration resulted in improved semen quality in cattle [8,28,32] and buffalo bulls [4,33,34]. Active sperm motility is essential to pass through the filters used in sperm separation.…”

“…The artificial insemination centers are a huge burden or an economic loss that is not insignificant, estimated at millions of $ due to the use of sperm from low-fertility bulls [7]. These losses are estimated at 3-4 $/day/bull in Iraq [8], which had negatively reflected in the low financial return of artificial insemination centers and breeders. So, improving semen quality is an important task to get the most benefit from geneticallysuperior bulls or/and have poor quality semen.…”

“…In semen separation techniques, motile sperms are separated from non-motile and debris [19]. Sperm separation techniques include dilution and washing [20], density-gradient centrifugation such as Percoll [21], Bovipure [22,23], Sill Select Plus [24], selective filters such as Sephadex and glass wool, and self-migration techniques such as the swim-up or swim-down technique [8,25,26,27,28]. Some techniques of semen separation from fresh semen or after thawing semen gave good results for bull semen characteristics [8,27,28,29].…”

Effect of magnetic field on productive and hysiological performance in broiler breeder

“…This study was conducted in the poultry house in the animal research station of the Department of Animal Production -College of Agriculture -University of Diyala, to study the effect of the magnetic field on some productive traits of Ross 308 broiler breeder hens. The first group represents control without treatment (T1) storage diluted semen for 24 hours at a temperature of 2°C under the influence of a magnetic field intensity of 803 gauss (The extender used in dilution was modified lake plus Turin according to (Hassan and Mohammad, 2021) represent T2; Treated fertile eggs with magnetic field with 250 gauss intensity for 72 hours. Treated the individual cages of the hens with magnetic field 250 gauss intensity during the experimental period (T4).…”