Effect of synthetic fragments of HIV protein immunodominant sites on human neutrophil oxygen metabolism

Zemskov, V. M.; Drukh, V. M.; Субботин, Сергей Митрофанович; Андреев, С. М.; Sidorova, M. V.; Vafina, M.G.; Tsvetkov, D. E.; Vorob'ev, A. A.

doi:10.1007/bf00847191

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“…114), and their spontaneous or induced (by 50 ng/ml phorbol ester (PMA) or zymosan) luminol-dependent chemiluminescence (CL) was studied using an LKB L1251 luminometer [1]. In addition, an original method was used: neutrophils from one subject were divided into two portions, 2x106 cells from one of them were Institute of Immunology, Ministry of Health and Medical Industry of Russia, Moscow {Presented by A.…”

Section: Methodsmentioning

confidence: 99%

Phagocyte activation with anti-ICAM-3 monoclonal antibodies

et al. 1996

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Monoclonal antibodies ICO-60 clustered as CDw50 (anti-ICAM-3) drastically boosted the chemiluminescence of human neutrophils, depending on the dose of antibodies, initial chemiluminescence, and individual human reactivity. The contact-adhesion interactions of neutrophils treated with ICO-60 and of intact neutrophils were associated with intensification of chemiluminescence. ICO-60 sharply increased the adhesion and aggregation of human neuroblasts and suppressed these phenomena induced by phorbol ester. The expression of ICAM-3 on neuroblasts is proposed. Key Words: neutrophils; neuroblasts; oxygen metabolism; anti-ICAM-3 antibodies (1C0-60)The functional activity of monoclonal antibodies (MAb) ICO-60 obtained by A. Yu. Baryshnikov, clustered as CDw50 (anti-ICAM-3), is analyzed. The ICAM-3 contraceptor for integrin molecule LFA-1 was recently discovered and is being intensively studied at present [3,4]. MATERIALS AND METHODSNeutrophils were isolated from venous blood of healthy donors and patients with the asthmatic triad in a Ficoll-Verograffin density gradient (C=I. 114), and their spontaneous or induced (by 50 ng/ml phorbol ester (PMA) or zymosan) luminol-dependent chemiluminescence (CL) was studied using an LKB L1251 luminometer [1]. In addition, an original method was used: neutrophils from one subject were divided into two portions, 2x106 cells from one of them were Institute of Immunology, Ministry of Health and Medical Industry of Russia, Moscow {Presented by A. A. Vorob'ev, Member of the Russian Academy of Medical Sciences} used untreated or pretreated with PMA or MAb ICO-60, after which 10 mM iodacetamide and then 100,000 intact neutrophils from the other portion were added, and CL was studied. In some experiments we studied CL of neutrophils treated with MAb ICO-60 in different dilutions. A neuroblast suspension was isolated by pipetting and washing from the brain of 8-9-week fetuses obtained during induced abortions. Adhesion of these neuroblasts to polystyrene and aggregation under the effect of PMA or MAb ICO-60, which were incubated for 30 min with the cells at 37~ were studied. In some experiments the cells were pretreated for 15 min with ICO-60, then washed on a centrifuge, and incubated for 30 min more. RESULTSICO-60 antibodies caused a potent activation of spontaneous CL of neutrophils, whereas MAb CDllb detecting the expression of Mac-1 integrin did not affect or even suppressed it (Fig. 1). It is noteworthy

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Section: Methodsmentioning

confidence: 99%