Effect of Systemic Oxytocin Administration on New Bone Formation and Distraction Rate in Rabbit Mandible

Altay, Berkan; Dede, Eda Çiftci; Özgül, Özkan; Atıl, Fethi; Koçyiğit, İsmail; Orhan, Kaan; Tekin, Umut; Korkusuz, Petek; Önder, M. Ercüment

doi:10.1016/j.joms.2020.03.005

Cited by 8 publications

(12 citation statements)

References 41 publications

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“…have reported that OXTR antagonism with atosiban resulted in an increased hard tissue formation in dental pulp in a dog pulpotomy model. Contrastingly, Altay et al 35 . have shown that systemic administration of OXT resulted in increased bone healing in a distraction osteogenesis model in rabbits, whereas Colli et al 36 .…”

Section: Discussionmentioning

confidence: 94%

“…Contrastingly, Altay et al 35 have shown that systemic administration of OXT resulted in increased bone healing in a distraction osteogenesis model in rabbits, whereas Colli et al 36 have reported that peripheral treatment with OXT promoted bone formation during the alveolar healing process after tooth extraction in 24-month-old female rats. Despite these seemingly contradictory findings, a recent review of the role of OXT and OXTR in bone metabolism has shown a general consensus that peripheral OXT participates in increasing bone mass and improves bone microstructure.…”

Section: Discussionmentioning

confidence: 96%

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Oxytocin alleviates periodontitis in adult rats

Dmytrenko,

Fernández‐Solari,

Correa

et al. 2024

J of Periodontal Research

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ObjectiveThe objective of the study was to evaluate the expression of oxytocin receptors in normal and inflamed gingiva, as well as the effects of systemic administration of oxytocin in bone loss and gum inflammatory mediators in a rat model of experimental periodontitis.Background DataCurrent evidence supports the hypothesis of a disbalance between the oral microbiota and the host's immune response in the pathogenesis of periodontitis. Increased complexity of the microbial biofilm present in the periodontal pocket leads to local production of nitrogen and oxygen‐reactive species, cytokines, chemokines, and other proinflammatory mediators which contribute to periodontal tissue destruction and bone loss. Oxytocin has been suggested to participate in the modulation of immune and inflammatory processes. We have previously shown that oxytocin, nitric oxide, and endocannabinoid system interact providing a mechanism of regulation for systemic inflammation. Here, we aimed at investigating not only the presence and levels of expression of oxytocin receptors on healthy and inflamed gingiva, but also the effects of oxytocin treatment on alveolar bone loss, and systemic and gum expression of inflammatory mediators involved in periodontal tissue damage using ligature‐induced periodontitis. Therefore, anti‐inflammatory strategies oriented at modulating the host's immune response could be valuable adjuvants to the main treatment of periodontal disease.MethodsWe used an animal model of ligature‐induced periodontitis involving the placement of a linen thread (Barbour flax 100% linen suture, No. 50; size 2/0) ligature around the neck of first lower molars of adult male rats. The ligature was left in place during the entire experiment (7 days) until euthanasia. Animals with periodontitis received daily treatment with oxytocin (OXT, 1000 μg/kg, sc.) or vehicle and/or atosiban (3 mg/kg, sc.), an antagonist of oxytocin receptors. The distance between the cement–enamel junction and the alveolar bone crest was measured in stained hemimandibles in the long axis of both buccal and lingual surfaces of both inferior first molars using a caliper. TNF‐α levels in plasma were determined using specific rat enzyme‐linked immunosorbent assays (ELISA). OXT receptors, IL‐6, IL‐1β, and TNF‐α expression were determined in gingival tissues by semiquantitative or real‐time PCR.ResultsWe show that oxytocin receptors are expressed in normal and inflamed gingival tissues in male rats. We also show that the systemic administration of oxytocin prevents the experimental periodontitis‐induced increased gum expression of oxytocin receptors, TNF‐α, IL‐6, and IL‐1β (p < .05). Furthermore, we observed a reduction in bone loss in rats treated with oxytocin in our model.ConclusionsOur results demonstrate that oxytocin is a novel and potent modulator of the gingival inflammatory process together with bone loss preventing effects in an experimental model of ligature‐induced periodontitis.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 96%

Oxytocin alleviates periodontitis in adult rats

Dmytrenko,

Fernández‐Solari,

Correa

et al. 2024

J of Periodontal Research

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“…In order to promote the healing of distraction osteogenesis, researchers in various countries have conducted a large number of experimental studies on it. Akçay found that the systemic application of vitamin E can stimulate the formation of new bone in rabbit distraction osteogenesis, thereby shortening the treatment time ( Akçay et al, 2019 ); Acikan found that systemic application of melatonin can increase the formation of new bone in distraction osteogenesis ( Acikan et al, 2018 ); Altay found that oxytocin can promote bone metabolism, increase new bone formation and promote bone healing in the distraction area ( Altay et al, 2020 ); McDonald applied sclerostin antibody (Scl-Ab) in the rat model of distraction osteogenesis. The results showed that Scl-Ab treatment could promote bone formation and lead to bone regeneration with larger volume and higher strength.…”

Section: Discussionmentioning

confidence: 99%

Rat bone marrow mesenchymal stem cells induced by rrPDGF-BB promotes bone regeneration during distraction osteogenesis

Zhang²,

Zhang³

et al. 2023

Front. Bioeng. Biotechnol.

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Background: In the clinical treatment of large bone defects, distraction osteogenesis can be used. However, some patients may suffer from poor bone regeneration, or even delayed healing or non-union. Problems with the aggregation and proliferation of primary osteoblasts, or problems with the differentiation of primary osteoblasts will lead to poor bone regeneration. Therefore, supplementing exogenous primary osteoblasts and growth factors when using distraction osteogenesis may be a treatment plan with great potential.Methods: Bone marrow mesenchymal stem cells (BMSCs) were extracted from rats and cultured. Subsequently, Recombinant Rat Platelet-derived Growth Factor BB (rrPDGF-BB) was used to induce bone marrow mesenchymal stem cells. At the same time, male adult rats were selected to make the right femoral distraction osteogenesis model. During the mineralization period, phosphate buffer salt solution (control group), non-induction bone marrow mesenchymal stem cells (group 1) and recombinant rat platelet-derived growth factor BB intervened bone marrow mesenchymal stem cells (group 2) were injected into the distraction areas of each group. Then, the experimental results were evaluated with imaging and histology. Statistical analysis of the data showed that the difference was statistically significant if p < 0.05.Results: After intervention with recombinant rat platelet-derived growth factor BB on bone marrow mesenchymal stem cells, the cell morphology changed into a thin strip. After the cells were injected in the mineralization period, the samples showed that the callus in group 2 had greater hardness and the color close to the normal bone tissue; X-ray examination showed that there were more new callus in the distraction space of group 2; Micro-CT examination showed that there were more new bone tissues in group 2; Micro-CT data at week eight showed that the tissue volume, bone volume, percent bone volume, bone trabecular thickness, bone trabecular number and bone mineral density in group 2 were the largest, and the bone trabecular separation in group 2 was the smallest. There was a statistical difference between the groups (p < 0.05); HE staining confirmed that group 2 formed more blood vessels and chondrocytes earlier than the control group. At 8 weeks, the bone marrow cavity of group 2 was obvious, and some of them had been fused.Conclusion: The study confirmed that injecting bone marrow mesenchymal stem cellsBB into the distraction space of rats can promote the formation of new bone in the distraction area and promote the healing of distraction osteogenesis.

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“…Tissue samples were fixed in 10% formaldehyde solution in phosphate buffer (pH 7.0) at room temperature and gradually decalcified in de Castro solution for 4 weeks at room temperature. 20 The samples were embedded in paraffin following dehydration and clearing in graded alcohols and xylenol in a fixed vacuum tissue processor. Serial sections of 3-4 μm thickness were taken from paraffin blocks on a sliding microtome (Leica, Weztlar, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…Consecutive hematoxylin eosin and Masson’s trichrome-stained sections revealed general morphology and quantification of new bone formation, respectively, under bright-field microscope (DM-6B, Leica). 20 Micrographs were transferred to a computer, and the new bone area (NBA) 21 and total defect area (TDA) in the segmentary defect area were quantitatively measured in square millimeters with the image analysis program (LAS v3, Leica, Germany). 20 The sections were evaluated based on the zone where new bone tissue was separated from the damaged bone ends and by measuring the length of the defect created ( Figure 5 ).…”

Section: Methodsmentioning

confidence: 99%

Adrenomedullin has no effect on segmental bone defect healing but increases bone mineral density in rat model

2023

AOTT

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Objective: This study aimed to investigate the effect of adrenomedullin on the healing of the segmental bone defect in a rat model. Methods: Thirty-six Wistar rats were randomly divided into 6 groups based on follow-up periods and administered a dose of adrenomedullin hormone. In each group, bilaterally, a 2-mm bone defect was created at the diaphysis of the radius. Sodium chloride solution was administered to sham groups 3 times a week for 4 and 8 weeks intraperitoneally. Adrenomedullin was administered to the study groups 3 times a week: 15 μg—4 weeks, 15 μg—8 weeks, 30 μg—4 weeks, and 30 μg—8 weeks, respectively. After euthanasia, the segmental defects were evaluated by histomorphometric [new bone area (NBA)] and microtomographic [bone volume (BV), bone surface (BS), and bone mineral density (BMD)] analyses. Results: Although the 4- and 8-week 15 μg administered study groups had higher NBA values than the other study and control groups, the histomorphometric analysis did not reveal any statistical difference between the control and study groups regarding NBA ( P > .05). In microtomographic analysis, BV was higher in the 15 μg 4-week group than 30 μg 4-week group (296.9 vs. 208.5, P = .003), and BS was lower in the 30 μg 4-week group than in the 4-week control group (695.5 vs. 1334.7, P = .005), but overall, no significant difference was found between the control and study groups ( P > .05). Despite these minor differences in histomorphometric and microtomographic criteria indicating new bone formation, the BMD values of the 15 μg 8-week study group showed a significant increase compared with the control group ( P = .001, respectively). Conclusion: Adrenomedullin positively affected BMD at 15 μg, but this study could not show healing in the segmental defect site at different dose regimens. Further studies are needed to assess its effects on bone tissue trauma.

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Effect of Systemic Oxytocin Administration on New Bone Formation and Distraction Rate in Rabbit Mandible

Cited by 8 publications

References 41 publications

Oxytocin alleviates periodontitis in adult rats

Oxytocin alleviates periodontitis in adult rats

Rat bone marrow mesenchymal stem cells induced by rrPDGF-BB promotes bone regeneration during distraction osteogenesis

Adrenomedullin has no effect on segmental bone defect healing but increases bone mineral density in rat model

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