Effect of Tape Stripping and Adjuvants on Immune Response After Intradermal DNA Electroporation

Vandermeulen, Gaëlle; Daugimont, Liévin; Richiardi, Hervé; Vanderhaeghen, Marie-Lise; Lecouturier, Nathalie; Ucakar, Bernard; Préat, Véronique

doi:10.1007/s11095-009-9885-3

Cited by 16 publications

(11 citation statements)

References 41 publications

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“…Skin GET can result in transfected cells in the epidermis, dermis, hypodermis, and even the muscle layer, dependent on the electrodes, injection techniques, animal species and plasmid designs used, for example whether tissue specific promoters are involved. GET with plate electrodes transfects cells to the mouse dermis [27], the epidermis of xenograft human skin [9], or both epidermis and dermis of rat [13]. GET with needle electrodes or needle arrays can reach the deep layers of the skin and result in transgenic cells in the dermis, epidermis, hypodermis and subcutaneous muscle layer of the mouse or rat [14], [28] or dermis of the pig [29].…”

Section: Discussionmentioning

confidence: 99%

Topical Gene Electrotransfer to the Epidermis of Hairless Guinea Pig by Non-Invasive Multielectrode Array

et al. 2013

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Topical gene delivery to the epidermis has the potential to be an effective therapy for skin disorders, cutaneous cancers, vaccinations and systemic metabolic diseases. Previously, we reported on a non-invasive multielectrode array (MEA) that efficiently delivered plasmid DNA and enhanced expression to the skin of several animal models by in vivo gene electrotransfer. Here, we characterized plasmid DNA delivery with the MEA in a hairless guinea pig model, which has a similar histology and structure to human skin. Significant elevation of gene expression up to 4 logs was achieved with intradermal DNA administration followed by topical non-invasive skin gene electrotransfer. This delivery produced gene expression in the skin of hairless guinea pig up to 12 to 15 days. Gene expression was observed exclusively in the epidermis. Skin gene electrotransfer with the MEA resulted in only minimal and mild skin changes. A low level of human Factor IX was detected in the plasma of hairless guinea pig after gene electrotransfer with the MEA, although a significant increase of Factor IX was obtained in the skin of animals. These results suggest gene electrotransfer with the MEA can be a safe, efficient, non-invasive skin delivery method for skin disorders, vaccinations and potential systemic diseases where low levels of gene products are sufficient.

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Section: Discussionmentioning

confidence: 99%

Topical Gene Electrotransfer to the Epidermis of Hairless Guinea Pig by Non-Invasive Multielectrode Array

et al. 2013

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“…Salvioli and colleagues as well demonstrated improved intradermal penetration of heat-killed microorganisms of the antituberculosis vaccine in infants (Salvioli et al, 1952). Although HYA was proven to increase the efficacy of vaccines when used with both traditional (Bergqvist, 1950;Salvioli et al, 1952;Tozuka et al, 1954) and plasmid DNA vaccines (McMahon et al, 2001;Vandermeulen et al, 2009;Potter and Heller, 2011), no one studied its role when used in combination with ET. As a consequence, we asked whether exogenous HYA is able to affect skeletal muscle and may influence the muscle inflammatory reaction when used alone or with ET.…”

Section: Introductionmentioning

confidence: 99%

Hyaluronidase Contributes to Early Inflammatory Events Induced by Electrotransfer in Mouse Skeletal Muscle

et al. 2013

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Electrotransfer of genes is one of the preferred strategies used to deliver plasmid DNA into skeletal muscle. In our experience, the combination of hyaluronidase (HYA) with electrotransfer (ET) of DNA vaccine enhances transfection of muscular fibers and increases expression of the encoded antigen. However, the contribution of HYA to the inflammatory reaction induced by ET, and its role in supporting ET adjuvancy, has never been investigated. We analyzed the events occurring in the first 2 weeks after electrotransfer to mouse muscle in the presence of HYA, to verify whether HYA contributes to the local inflammatory response induced by ET. Our results demonstrate that HYA amplifies the ET effect in terms of inflammatory cell recruitment enhancing the early release of interleukin (IL)-1β, tumor necrosis factor-α, and IL-6 cytokines. In contrast, HYA does not induce helper T cell type 1 and 2 cytokine production, confirming that the DNA vaccine is indispensable to induce mediators of antigen-specific immune responses. We observed inflammatory cell migration in the muscle treated with HYA plus ET in a time window between days 4 and 7 after cytokine induction. These observations are important in the choice of prime-boost intervals for optimizing ET-based DNA vaccination protocols. Because HYA contributes to vaccine spread and enhances the proinflammatory effect of ET in muscle we strongly support the use of HYA to potentiate DNA vaccine efficacy.

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“…The results of these studies demonstrated that an approximately 5-fold increase in expression of the gene of interest could be achieved after hyaluronidase muscle pretreatment (McMahon et al, 2001;Mennuni et al, 2002;Molnar et al, 2004). However, hyaluronidase did not improve transfection in skin (Vandermeulen et al, 2009). In tumors, where interstitial pressure is uniformly elevated and convection in the tumor tissue is negligible, the distribution of molecules relies on passive diffusion, which is mostly ineffective.…”

Section: Introductionmentioning

confidence: 99%

“…These studies were done in muscle and skin (McMahon et al, 2001;Mennuni et al, 2002;Vandermeulen et al, 2009). The transfection efficiency was increased only in muscle.…”

mentioning

confidence: 99%

Hyaluronidase and Collagenase Increase the Transfection Efficiency of Gene Electrotransfer in Various Murine Tumors

et al. 2012

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One of the applications of electroporation/electropulsation in biomedicine is gene electrotransfer, the wider use of which is hindered by low transfection efficiency in vivo compared with viral vectors. The aim of our study was to determine whether modulation of the extracellular matrix in solid tumors, using collagenase and hyaluronidase, could increase the transfection efficiency of gene electrotransfer in histologically different solid subcutaneous tumors in mice. Tumors were treated with enzymes before electrotransfer of plasmid DNA encoding either green fluorescent protein or luciferase. Transfection efficiency was determined 3, 9, and 15 days posttransfection. We demonstrated that pretreatment of tumors with a combination of enzymes significantly increased the transfection efficiency of electrotransfer in tumors with a high extracellular matrix area (LPB fibrosarcoma). In tumors with a smaller extracellular matrix area and less organized collagen lattice, the increase was not so pronounced (SA-1 fibrosarcoma and EAT carcinoma), whereas in B16 melanoma, in which only traces of collagen are present, pretreatment of tumors with hyaluronidase alone was more efficient than pretreatment with both enzymes. In conclusion, our results suggest that modification of the extracellular matrix could improve distribution of plasmid DNA in solid subcutaneous tumors, demonstrated by an increase in transfection efficiency, and thus have important clinical implications for electrogene therapy.

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Effect of Tape Stripping and Adjuvants on Immune Response After Intradermal DNA Electroporation

Abstract: Tape stripping is able to increase the Th1 immune response against both DNA and protein vaccines. Therefore, tape stripping appears to have interesting adjuvant effect on intradermal vaccination.

Cited by 16 publications

References 41 publications

Topical Gene Electrotransfer to the Epidermis of Hairless Guinea Pig by Non-Invasive Multielectrode Array

Topical Gene Electrotransfer to the Epidermis of Hairless Guinea Pig by Non-Invasive Multielectrode Array

Hyaluronidase Contributes to Early Inflammatory Events Induced by Electrotransfer in Mouse Skeletal Muscle

Hyaluronidase and Collagenase Increase the Transfection Efficiency of Gene Electrotransfer in Various Murine Tumors

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