Effect of TDZ and 2, 4-D on peanut somatic embryogenesis and in vitro bud development

Joshi, M. S.; Sujatha, K.; Hazra, Sulekha

doi:10.1007/s11240-008-9390-0

Cited by 23 publications

(9 citation statements)

References 13 publications

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“…A successful regeneration of the subdivided rosettes from TDZ was observed when they were transferred to MS culture media without growth regulators, showing that subcultivation in these conditions is crucial for later development of these explants (Khalafalla and Hattori, 1999;Joshi et al, 2008). Although Prathanturarug et al (2005) has suggest that a second treatment with TDZ may increase culture multiplication, the results from our work do not corroborate with this possibility, since explants regeneration in culture media supplemented with high doses of TDZ was very low.…”

Section: Cultured In Vitrocontrasting

confidence: 55%

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An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L.

Bianchetti¹,

Resende²,

Pacheco³

et al. 2017

Afr. J. Biotechnol.

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This work aimed to develop a protocol for the in vitro establishment, multiplication, rooting and ex vitro acclimatization of Mimosa pudica L., a species used in folk medicine and with pharmacological activity. Aseptic cultures were established from seeds inoculated in MS medium, without growth regulators, followed by an in vitro stabilization phase in culture medium supplemented with 2.22 μM BAP. The cultures were transferred to MS medium supplemented with different cytokinins, combined or not with an auxin, aiming its large-scale propagation. The culture medium supplemented with 5 µM BAP plus 0.5 µM NAA provided the highest multiplication rate and top quality plantlets. The combination of 0.6 µM TDZ plus 0.05 µM NAA resulted in higher multiplication rates than in response to combination of BAP plus NAA, although the subsequent maintenance of the cultures in a medium without growth regulators has resulted in low regenerative response. In vitro rooting of micro-cuttings was high even in the absence of auxins. Over 90% of plantlets transferred to the greenhouse survived after the acclimatization phase. Acclimatized plants presented normal vegetative and reproductive development. The procedures established in the present study allow a massive production of M. pudica plants for further pharmacological studies.

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Section: Cultured In Vitrocontrasting

confidence: 55%

“…Low concentrations of TDZ were effective in inducing multiple in vitro shoots in peanuts cultures (Joshi et al, 2008). In Lens culinaris Medik., 0.5 µM TDZ also stimulate in vitro shoots formation (Chhabra et al, 2008).…”

Section: Cultured In Vitromentioning

confidence: 99%

An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L.

Bianchetti¹,

Resende²,

Pacheco³

et al. 2017

Afr. J. Biotechnol.

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“…However, later studies have demonstrated that TDZ, unlike traditional cytokinins, is capable of fulfilling both the cytokinin and auxin requirements of various regenerative responses of many different plant species Hutchinson et al 1996;Murthy et al 1998;Sanikhani et al 2006). Although TDZ has not been used in regeneration of petunia, it has proven to be highly efficient for shoot regeneration in various plant species (Akasaka et al 2000;Sharma et al 2007;Dimech et al 2007;Yucesan et al 2007;Joshi et al 2008;Liu and Pijut 2008;Corredoira et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Thidiazuron: an efficient plant growth regulator for enhancing Agrobacterium-mediated transformation in Petunia hybrida

Thirukkumaran

Ntui

Khan

et al. 2009

Plant Cell Tiss Organ Cult

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Efficient shoot regeneration and Agrobacterium-mediated genetic transformation systems were developed for Petunia hybrida cv. Mitchell. Leaf explants of petunia were cultured on Murashige and Skoog (MS) medium with different concentrations of thidiazuron (TDZ) without auxin. The highest frequency of shoot regeneration (52.1%) and mean number of shoots per explant (4.1) were obtained on medium containing 2 mg l -1 TDZ. Leaf explants inoculated with Agrobacterium tumefaciens strain EHA101/pIG121Hm harboring ß-glucuronidase (uidA) and hygromycin resistance genes developed putative transformant shoots. The highest frequency of shoot regeneration (22.5%) and mean number of transformant shoots per explant (2.4) were obtained on a selection medium consisting of the above described regeneration medium and containing 25 mg l -1 hygromycin as the selection agent. Approximately 95% of putative transformant shoots expressed the uidA gene following histochemical ß-glucuronidase (GUS) assay. These were confirmed to be transgenic by PCR analysis and Southern blot hybridization.

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“…In Arachis hypogaea a good embryo conversion to plantlets was recorded at 0.18 M sucrose and 9.08 lM TDZ. This combination triggered the morphogenetic activity in the plumule that was not observed when other combinations were used (Joshi et al 2008).…”

Section: Discussionmentioning

confidence: 88%

Creation of novel interspecific-interploid Hylocereus hybrids (Cactaceae) via embryo rescue

2012

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In this study we developed a technique for rescuing embryos at a very early developmental stage following interspecific-interploid crosses between Hylocereus sp. Controlled hand pollinations were performed between the tetraploid H. megalanthus (Vaup.) Bauer as the female parent and either diploid H. monacanthus (Lem.) Britton et Rose or H. undatus (Haw.) Britton et Rose as the male parent. The fertilized ovules were excised from ovaries 10 or 30 days after pollination (DAP). Pollinated ovules containing the funiculi and placental tissue and immature embryos were placed on half-strength basal Murashige and Skoog (MS) medium containing 680 lM glutamine, 0.54 lM a-naphthaleneacetic acid, and 0.45 lM thidiazuron and supplemented with 0.00, 0.09, 0.17 or 0.26 M sucrose concentrations. The best ovule response was recorded at 30 DAP, and the most prolific callus formation was observed at 10 DAP. Callus formation was observed in most of the treatments using whole ovules but not in the isolated immature embryos. The calli were mucilaginous or compact, transparent and friable, but they did not form embryogenic structures. Embryo development was significantly affected by the sucrose concentration, and the best results were obtained with 0.17 M sucrose. More than 70 % of the obtained hybrids were successfully hardened off and transplanted in soil where they grew normally. Ploidy level analyses of 77 putative hybrids exposed diploid, triploid, tetraploid, and higher than tetraploid levels. Among those hybrids studied, 22 progenies were randomly chosen for amplified fragment length polymorphism analysis, and all were identified as genuine hybrids. The technology described here is an additional stage in the breeding program for Hylocereus species resulting in novel, interspecific hybrids obtained using the embryo rescue technique. Keywords Amplified fragment length polymorphism (AFLP) Á Flow cytometry Á a-Naphthaleneacetic acid Á Ovule culture Á Thidiazuron Á Vine cacti Abbreviations AFLP Amplified fragment length polymorphism BGU Ben-Gurion University of the Negev DAP Days after pollination IE Immature embryos MS Murashige and Skoog NAA a-Naphthaleneacetic acid NIB Nuclei isolation buffer TDZ Thidiazuron WO Whole ovules

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Effect of TDZ and 2, 4-D on peanut somatic embryogenesis and in vitro bud development

Cited by 23 publications

References 13 publications

An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L.

An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L.

Thidiazuron: an efficient plant growth regulator for enhancing Agrobacterium-mediated transformation in Petunia hybrida

Creation of novel interspecific-interploid Hylocereus hybrids (Cactaceae) via embryo rescue

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