Effect of temperature and glucose concentration on the N-butanoyl-l-homoserine lactone production by Aeromonas hydrophila

Medina-Martínez, María S.; Uyttendaele, Mieke; Demolder, V.; Debevere, Johan

doi:10.1016/j.fm.2005.09.010

Cited by 17 publications

(9 citation statements)

References 31 publications

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“…Maximal AHL synthesis is observed at the optimal growth temperature (24 °C) and is directly correlated with expI transcripts; at 12–15 °C, AHL production is reduced whereas pectate lyase production is optimal, suggesting a thermoregulation occurring downstream quorum signalling (Latour et al , 2007). The influence of temperature on AHL synthesis has also been studied in the food‐borne human opportunistic pathogen Aeromonas hydrophila that regulates exoprotease production and biofilm development through QS; the concentration of C 4 ‐HSL is lower at 12 °C than at 22 or 30 °C (the optimal growth temperature) despite reaching dense populations, indicating that 12 °C is not inhibitory to C 4 ‐HSL production and that quorum signalling might occur under real food conditions (Medina‐Martinez et al , 2006).…”

Section: Environmental Factors Affecting the Synthesis Stability DImentioning

confidence: 99%

“…carotovora , the nature of the carbon source (and also the temperature of growth) influences carbepenem production by modulating the level of carI transcription; the concentration of 3oxo,C 6 ‐HSL is high, intermediate or low in, respectively, a sucrose, a glucose or a glycerol‐grown culture (McGowan et al , 2005). In A. hydrophila , C 4 ‐HSL synthesis occurs in Luria–Bertani broth supplemented with 0.1% and 0.5% glucose whereas no synthesis is detected in 1% glucose (Medina‐Martinez et al , 2006); the QS regulation of exoproteases would allow this bacterium to use alternative carbon sources when the medium becomes deficient in glucose. In R. leguminosarum , where QS notably regulates transfer of a symbiotic plasmid, the quantity and the ratio of each AHL are also affected by the composition of the growth medium (Lithgow et al , 2001).…”

Section: Environmental Factors Affecting the Synthesis Stability DImentioning

confidence: 99%

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Cellâcell signalling in bacteria: not simply a matter of quorum

Boyer

Wisniewski‐Dyé

2009

FEMS Microbiology Ecology

191

163

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Bacterial signalling known as quorum sensing (QS) relies on the synthesis of autoinducing signals throughout growth; when a threshold concentration is reached, these signals interact with a transcriptional regulator, allowing the expression of specific genes at a high cell density. One of the most studied intraspecies signalling is based on the use of N-acyl-homoserine lactones (AHL). Many factors other than cell density were shown to affect AHL accumulation and interfere with the QS signalling process. At the cellular level, the genetic determinants of QS are integrated in a complex regulatory network, including QS cascades and various transcriptional and post-transcriptional regulators that affect the synthesis of the AHL signal. In complex environments where bacteria exist, AHL do not accumulate at a constant rate; the diffusion and perception of the AHL signal outside bacterial cells can be compromised by abiotic environmental factors, by members of the bacterial community such as AHL-degrading bacteria and also by compounds produced by eukaryotes acting as an AHL mimic or inhibitor. This review aims to present all factors interfering with the AHL-mediated signalling process, at the levels of signal production, diffusion and perception.

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Section: Environmental Factors Affecting the Synthesis Stability DImentioning

confidence: 99%

Section: Environmental Factors Affecting the Synthesis Stability DImentioning

confidence: 99%

Cellâcell signalling in bacteria: not simply a matter of quorum

Boyer

Wisniewski‐Dyé

2009

FEMS Microbiology Ecology

191

163

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“…The proteolytic activity of some extracellular enzymes of A. hydrophila has been recognized and it is considered to play a major role in the virulence and pathogenicity of this bacterium [ 26 ]. Besides, Vivas [ 27 ] showed that this microorganism can produce and secrete proteases able to cleave milk proteins.…”

Section: Resultsmentioning

confidence: 99%

Quorum Sensing and Spoilage Potential of Psychrotrophic Enterobacteriaceae Isolated from Milk

Martins

Pinto

Riedel

et al. 2018

BioMed Research International

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The 16S rDNA of six psychrotrophic Enterobacteriaceae isolated from cold raw milk were sequenced and the isolate 039 was identified as Pantoea sp., isolates 059, 068, and 071 were identified as Hafnia alvei, 067 was identified as Enterobacter sp., and 099 was identified as Aeromonas hydrophila. They presented different spoilage potentials in milk with A. hydrophila 099 being the most deteriorative. Only Pantoea sp. 039 was not able to induce the quorum sensing monitor strains of acyl homoserine lactones (AHLs). The halI gene, which encodes the AHL synthase in H. alvei, was identified in the isolates 059, 067, 068, and 071. After initial sequencing characterization and cloning, this gene showed its function by the heterologous synthesis of N-hexanoyl-DL-homoserine lactone and N-3-oxohexanoyl-L-homoserine lactone in Escherichia coli. In addition to producing AHLs, A. hydrophila 099 produced AI-2 in higher level than the assay's positive control Vibrio harveyi BB120. Therefore, Enterobacteriaceae strains isolated from cooled raw milk produce a rich array of signaling molecules that may influence bacterial traits in the milk environment.

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“…Based on TLC profiling we found that BHL and HHL were produced by waterborne A. hydrophila. It has been reported that environmental conditions determine the AHL production in A. hydrophila, Medina-Martinez et al [18,19] reported that environmental factors (temperature and composition of medium (glucose concentration)) affect AHLs production. Styp von Rekowski et al [20] results shown that AHL-mediated QS was not required for A. hydrophila during colonization and degradation of organic particles in lake water microcosms.…”

Section: Discussionmentioning

confidence: 99%

Production of N-acyl Homoserine Lactones and Virulence Factors of Waterborne Aeromonas hydrophila

Chu

Liu

Jiang³

et al. 2013

Indian J Microbiol

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Aeromonads are inhabitants of aquatic ecosystems and are described as being involved in intestinal disturbances and other infections. The purpose of this study was to investigate the production of N-acyl-homoserine lactone (AHL) signal molecules and some virulence factors, including hemolysins, proteases, extracellular nucleases production and cytotoxicity by waterborne Aeromonas hydrophila. A total of 24 strains isolated from fresh-water or diseased fish were used in the study. The majority A.hydrophila strains produce two AHL molecules (21/24), one is N-butanoyl homoserine lactone (BHL), and the other is N-hexanoyl homoserine lactone (HHL) according to thin-layer chromatography analysis. Among the virulence factors tested, more than 83 % of the isolates produced β haemolysin when inoculated on sheep blood agar, only 50 % of the isolates displayed DNase activity, 75 % of the isolates shown proteolytic activity on skimmed milk plate, and cytotoxic activity was detected in 20 of 24 of the isolates. The strains producing AHLs possessed one or more virulence factors. In conclusion, the production of quorum sensing signal molecules is common among the strains that we examined, and there seems to some relationships between quorum sensing signal production and virulence factors in A. hydrophila.

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Effect of temperature and glucose concentration on the N-butanoyl-l-homoserine lactone production by Aeromonas hydrophila

Cited by 17 publications

References 31 publications

Cellâcell signalling in bacteria: not simply a matter of quorum

Cellâcell signalling in bacteria: not simply a matter of quorum

Quorum Sensing and Spoilage Potential of Psychrotrophic Enterobacteriaceae Isolated from Milk

Production of N-acyl Homoserine Lactones and Virulence Factors of Waterborne Aeromonas hydrophila

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Effect of temperature and glucose concentration on the N-butanoyl-l-homoserine lactone production by Aeromonas hydrophila

Cited by 17 publications

References 31 publications

Cellâcell signalling in bacteria: not simply a matter of quorum

Cellâcell signalling in bacteria: not simply a matter of quorum

Quorum Sensing and Spoilage Potential of Psychrotrophic Enterobacteriaceae Isolated from Milk

Production of N-acyl Homoserine Lactones and Virulence Factors of Waterborne Aeromonas hydrophila

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Cellâcell signalling in bacteria: not simply a matter of quorum

Cellâcell signalling in bacteria: not simply a matter of quorum