Effect of temperature on 3,4-Methylenedioxypyrovalerone (MDPV)-induced metabolome disruption in primary mouse hepatic cells

Araújo, Ana Margarida; Bastos, Maria de Lourdes; Carvalho, Félix; Pinho, Paula Guedes de; Carvalho, Márcia

doi:10.1016/j.tox.2020.152503

Cited by 8 publications

(4 citation statements)

References 67 publications

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“…[47][48][49] It was found that liver cells (PMH) mice treated toxic doses of 3,4-Methylenedioxypyrovalerone caused dysregulation in these pathways that were obtained for our study, except for arginine biosynthesis. 50 In the study, a rapid and sensitive GC-QqQ-MS/ MS method was developed and validated for quantitative 19 amino acids in serum, which represent important progress for the analysis of amino acids in biological samples. Our results indicated that the analyses of PCA and OPLS-DA can differentiate between TP-induced and control groups just 24 h after TP administration.…”

Section: Discussionmentioning

confidence: 99%

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Metabolic profiling of 19 amino acids in triptolide-induced liver injured rats by gas chromatography-triple quadrupole mass spectrometry

et al. 2021

Hum Exp Toxicol

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The liver is an important organ for amino acid metabolism, and its damage can be reflected in the changes of amino acid level in the body. Triptolide (TP) has broad anti-inflammatory and anti-tumor activities, but its clinical application is limited due to hepatotoxicity. In this work, a simple, accurate and sensitive gas chromatography-triple quadrupole mass spectrometry (GC-QqQ-MS/MS) method was developed and validated for evaluating the serum levels of amino acids from control and TP-induced liver injured rats, and chemometric analysis was employed for amino acid metabolic profiles analysis. It was found that 11 amino acids showed significant changes after TP administration, and they were mainly involved in 5 metabolic pathways that are phenylalanine, tyrosine and tryptophan biosynthesis, alanine, aspartate and glutamate metabolism, glutamine and glutamate metabolism, phenylalanine metabolism and arginine biosynthesis. Five amino acids including tyrosine, glutamine, glutamic acid, tryptophan and alanine were identified as biomarkers of TP hepatotoxicity by further analysis. These results indicated that the novel amino acid metabolic profiling study based on the GC-QqQ-MS/MS provided not only exact concentrations of serum amino acids, but also a prospective methodology for evaluation of chemically induced liver injury.

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Section: Discussionmentioning

confidence: 99%

“…47–49 It was found that liver cells (PMH) mice treated toxic doses of 3,4-Methylenedioxypyrovalerone caused dysregulation in these pathways that were obtained for our study, except for arginine biosynthesis. 50…”

Section: Discussionmentioning

confidence: 99%

Metabolic profiling of 19 amino acids in triptolide-induced liver injured rats by gas chromatography-triple quadrupole mass spectrometry

et al. 2021

Hum Exp Toxicol

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“…GC-MS techniques can be utilized, for example, for the evaluation of certain metabolic disruptions caused by various toxicants [86]. Thus, the high sensitivity of GC-MS was useful for investigating the effect of different concentration levels (toxic and subtoxic) of 3,4-methylenedioxypyrovalerone on the metabolic profile of primary mouse hepatocytes, under normothermic and hyperthermic conditions, providing new insights into the mechanism of hepatotoxicity induced by this cathinone derivative as well as the higher risks occurring under hyperthermic conditions [87]. In another study, GC-MS has been used to analyze the metabolomic changes in the rat liver after chlorpyriphos, cadmium, and their mixtures treatment [88].…”

Section: Gc Determination Of Toxicants In Biological Matricesmentioning

confidence: 99%

Recent Applications of Gas Chromatography in Bioanalysis

David¹,

Moldoveanu²

2023

Novel Aspects of Gas Chromatography and Chemometrics

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Bioanalysis involves a broad range of chemical analyzes. These analyzes include that of biotics, such as natural components of living organisms, as well as xenobiotics, such as drugs and their metabolites in biological systems. Because many biotics and xenobiotics are not volatile molecules, the main technique for bioanalysis is high-performance liquid chromatography (HPLC) and the limitation of GC utilization is caused by the fact that GC is applicable only to volatile samples. However, gas chromatography (GC) in particular coupled with mass spectrometry (MS) as detection is also a very useful technique in bioanalysis. A considerable number of analytes in bioanalysis are volatile or can be made volatile following, for example, derivatization. As a result, GC (and GC/MS) are commonly utilized for the analysis of biotics, such as amino acids, fatty acids, various metabolites in biological fluids, and in particular of a large number of xenobiotics, such as drugs, drug metabolites, toxicants, and certain metabolic compounds caused by toxicants. The chapter will present progress in the GC methodology for extending its applicability to bioanalysis and will provide a review of more recent applications.

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“…Phosphate buffered saline (PBS), 0.17 mol L -1 , pH 7.4, was prepared using sodium phosphate dibasic and potassium phosphate monobasic, all obtained from Sigma-Aldrich. MDPV hydrochloride was purchased online (www.sensearomatics.eu) and was fully characterized by MS and elemental analysis (shown in the supplementary data of a recent publication [63]). The analytical data were consistent with the expected structure, with the salt having a purity of 99.5%, the salt consisted in a racemic (R and S enantiomers) mixture.…”

Section: Chemicals and Samplesmentioning

confidence: 99%

3,4-Methylenedioxypyrovalerone (MDPV) Sensing Based on Electropolymerized Molecularly Imprinted Polymers on Silver Nanoparticles and Carboxylated Multi-Walled Carbon Nanotubes

et al. 2021

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3,4-methylenedioxypyrovalerone (MDPV) is a harmful and controlled synthetic cathinone used as a psychostimulant drug and as sport-enhancing substance. A sensor was developed for the direct analysis of MDPV by transducing its oxidation signal by means of an electropolymerized molecularly imprinted polymer (e-MIP) built in-situ on the screen-printed carbon electrode’s (SPCE) surface previously covered with multi-walled carbon nanotubes (MWCNTs) and silver nanoparticles (AgNPs). Benzene-1,2-diamine was used as the functional monomer while the analyte was used as the template monomer. Each step of the sensor’s development was studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in a solution containing ferricyanide, however no redox probe was required for the actual MDPV measurements. The interaction between the poly(o-phenylenediamine) imprinted polymer and MDPV was studied by density-functional theory (DFT) methods. The SPCE-MWCNT-AgNP-MIP sensor responded adequately to the variation of MDPV concentration. It was shown that AgNPs enhanced the electrochemical signal by around a 3-fold factor. Making use of square-wave voltammetry (SWV) the developed sensor provided a limit of detection (LOD) of 1.8 μmol L–1. The analytical performance of the proposed sensor paves the way to the development of a portable device for MDPV on-site sensing to be applied in forensic and doping analysis.

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Effect of temperature on 3,4-Methylenedioxypyrovalerone (MDPV)-induced metabolome disruption in primary mouse hepatic cells

Cited by 8 publications

References 67 publications

Metabolic profiling of 19 amino acids in triptolide-induced liver injured rats by gas chromatography-triple quadrupole mass spectrometry

Metabolic profiling of 19 amino acids in triptolide-induced liver injured rats by gas chromatography-triple quadrupole mass spectrometry

Recent Applications of Gas Chromatography in Bioanalysis

3,4-Methylenedioxypyrovalerone (MDPV) Sensing Based on Electropolymerized Molecularly Imprinted Polymers on Silver Nanoparticles and Carboxylated Multi-Walled Carbon Nanotubes

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