Effect of temperature on immune response of Japanese flounder (Paralichthys olivaceus) to inactivated lymphocystis disease virus (LCDV)

Xu, Guojing; Sheng, Xiuzhen; Xing, Jing; Zhan, Wenbin

doi:10.1016/j.fsi.2010.11.026

Cited by 67 publications

(24 citation statements)

References 25 publications

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“…The homogenate was centrifuged at 14,000 × g for 30 min at 4 • C, and the supernatant was collected and stored at −70 • C until use. The leucocytes of different organs were isolated according to the procedure developed by Xu et al (2011).…”

Section: Samplingmentioning

confidence: 99%

“…Previous researches demonstrated that the antigen uptake and immune efficacy would be influenced by many factors, such as temperature, fish age, nutritional status, type of vaccine, physio-chemical characteristics of vaccine solution and immersion formulation (Xu et al, 2011;Soto et al, 2014;Tatner and Horne, 1985). Among these factors, vaccine concentration and immersion time were proved to largely influence the immune efficacy.…”

Section: Introductionmentioning

confidence: 99%

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Immune response of flounder (Paralichthys olivaceus) was associated with the concentration of inactivated Edwardsiella tarda and immersion time

Tang

Sheng

et al. 2015

Veterinary Immunology and Immunopathology

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Section: Samplingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immune response of flounder (Paralichthys olivaceus) was associated with the concentration of inactivated Edwardsiella tarda and immersion time

Tang

Sheng

et al. 2015

Veterinary Immunology and Immunopathology

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“…In addition, a study demonstrated that this acquired immunity, determined as immunoglobulin (IgM)‐positive cells and circulating IgM levels, was optimal at 21 °C and depressed at not natural temperatures for flounders (Xu et al . ).…”

Section: Introductionmentioning

confidence: 97%

“…Thus, several works have shown the protection conferred in flounder by vaccines against LCDV towards the generation of specific antibodies (Tian, Sun & Chen 2008a;Tian et al 2008b;Tian & Yu 2011). In addition, a study demonstrated that this acquired immunity, determined as immunoglobulin (IgM)-positive cells and circulating IgM levels, was optimal at 21°C and depressed at not natural temperatures for flounders (Xu et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the gilthead seabream (Sparus aurata L.) immune response under a natural lymphocystis disease virus outbreak

Cordero

Cuesta

Meseguer

et al. 2016

Journal of Fish Diseases

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Lymphocystis or lymphocystis disease virus (LCDV) is distributed worldwide and affects many fresh and marine water fish species. LCDV is commonly found in aquaria fish species but also in farmed fish species, among them the gilthead seabream (Sparus aurata L.). The immune status of gilthead seabream (S. aurata) specimens under a natural outbreak of LCDV was studied. The replication of the virus was demonstrated in infected fish, but not in control fish. The results showed decreased total serum IgM levels and increased innate cellular immune response (peroxidase and respiratory burst activities) of head kidney leucocytes in LCDV-infected fish, compared to the values obtained in uninfected specimens. In addition, transcription of antiviral genes (ifn and irf3) was down-regulated in the skin of LCDV-positive fish as well as genes involved in cellular immunity (csf1r, mhc2a, tcra and ighm) that were down-regulated in skin and head kidney of infected fish. By contrast, the transcription of nccrp1 was up-regulated in head kidney after LCDV infection. These present results show that head kidney leucocytes are activated to encounter the virus at the sites of replication.

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“…However, at higher temperatures (25° and 30°C) a robust and protective immune response was observed. In Japanese flounder ( Paralichthys olivaceus ) intraperitoneally vaccinated with inactivated virus, maximum immunoglobulin production was found in fish kept at 21°C; lower (9° to 15°C) or higher temperatures (26°C) had a deleterious effect on the production of circulating immunoglobulin (16). In fact, water temperature below 15°C decreases immunoglobulin production in several other fish species such as sea bass ( Dicentrarchus labrax ), Atlantic halibut ( Hippoglossus hippoglossus ), summer flounder ( Paralichthys dentatus ), Japanese flounder, Nile tilapia and carp ( Cyprinus carpio ) (17) when compared with higher temperatures (18° to 23°C).…”

Section: Resultsmentioning

confidence: 99%

Increased immunoglobulin production in silver catfish (Rhamdia quelen) exposed to agrichemicals

Kreutz

Pavan

Alves

et al. 2014

Braz J Med Biol Res

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Fish vaccination has been increasingly exploited as a tool to control pathogen infection. The production of immunoglobulin following vaccination might be affected by several factors such as management procedures, water temperature, and the presence of xenobiotics. In the present study, we aimed to investigate the kinetics of immunoglobulin production in silver catfish (Rhamdia quelen) inoculated with inactivated Aeromonas hydrophila and kept at two different water temperatures (17.4±0.4° or 21.3±0.3°C). The effect of a second antigen inoculation and exposure of fish to sublethal concentrations of the herbicides atrazine and glyphosate at 10% of the lethal concentration (LC50-96h) on specific serum antibodies were also investigated. Antibodies to A. hydrophila were detected as early as 7 days post-inoculation and increased steadily up to 35 days. The kinetics of antibody production were similar in fish kept at 17.4±0.4° and 21.3±0.3°C, and reinoculation of antigen at 21 days after priming failed to increase specific antibody levels. Intriguingly, we found that, in fish exposed to atrazine and glyphosate, the secretion of specific antibodies was higher than in non-exposed inoculated fish. These findings are important for the design of vaccines and vaccination strategies in Neotropical fish species. However, because atrazine and glyphosate are widespread contaminants of soil and water, their immune-stimulating effect could be harmful, in that fish living in herbicide-contaminated water might have increased concentrations of nonspecific antibodies that could mediate tissue injury.

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Effect of temperature on immune response of Japanese flounder (Paralichthys olivaceus) to inactivated lymphocystis disease virus (LCDV)

Cited by 67 publications

References 25 publications

Immune response of flounder (Paralichthys olivaceus) was associated with the concentration of inactivated Edwardsiella tarda and immersion time

Immune response of flounder (Paralichthys olivaceus) was associated with the concentration of inactivated Edwardsiella tarda and immersion time

Characterization of the gilthead seabream (Sparus aurata L.) immune response under a natural lymphocystis disease virus outbreak

Increased immunoglobulin production in silver catfish (Rhamdia quelen) exposed to agrichemicals

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