Effect of temperature on precision of retention measurements in liquid chromatography

Gilpin, R. K.; Sisco, W.R.

doi:10.1016/s0021-9673(00)81419-3

Cited by 56 publications

(11 citation statements)

References 31 publications

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“…Linear van’t Hoff plots involving the retention factor (ln k ' versus 1/T) were observed when the eluent is the mixture of equal volumes of 2-propanol and methanol (Figure 2). The plots of R,R -Koga base and meso-Koga base are almost parallel and have positive slopes with negative intercepts, exhibiting minor differences in selectivity as a function of operating temperature [13,14,15]. The plot of S,S -Koga base has a smaller positive slope and negative intercept in comparison with R,R - or meso- Koga base, demonstrating that retention of analytes and enantiomeric and diastereomeric selectivity decrease with increasing temperature.…”

Section: Resultsmentioning

confidence: 99%

Influence of Temperature on the Enantioselectivity of Koga Tetraamines on Amylose Chiral Stationary Phases

Guo

Sun

2013

Molecules

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Enantioseparation is largely based on the formation of transitional complexes, the solvation species, the stationary phase configurations or the diastereomeric complexes formed by analytes and the chiral stationary phase. Temperature and the chemical nature and composition of the eluent play significant roles during that process. In this study; unique temperature-induced behaviors were observed during the enantioseparation of Koga tetraamines, also known as Koga bases, on polysaccharide chiral stationary phases, in which van't Hoff plots were acquired over a temperature range of 10 °C to 40 °C with 5 °C increments. Koga bases were eluted by a mixture of methanol and 2-propanol with 0.03% triethylamine as a modifier. The van't Hoff plots are linear in the case of eluent containing equal volumes of methanol and 2-propanol. Increasing 2-propanol concentration from 50% to 85% in volume led to non-linear van't Hoff plots over the entire temperature range studied. Examination of the individual non-linear plots revealed two linear regions of 10 °C-20 °C and 20 °C-40 °C. Transition from one linear region to the other at 20 °C indicates alterations of chiral stationary phase conformation and/or enantioseparation mechanism as a result of temperature changes.

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Section: Resultsmentioning

confidence: 99%

Influence of Temperature on the Enantioselectivity of Koga Tetraamines on Amylose Chiral Stationary Phases

Guo

Sun

2013

Molecules

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“…The slurry method was applied to pack the obtained SiO 2 -N(C18) 4 into HPLC columns [23]. Briefly, a slurry of SiO 2 -N(C18) 4 (2.30 g) was made with 30 mL of isopropyl alcohol by stirring for at least 30 min and then placed in a dynamic reservoir system.…”

Section: Packing Of the Columnmentioning

confidence: 99%

Synthesis of a stationary phase based on silica modified with branched octadecyl groups by Michael addition and photoinduced thiol-yne click chemistry for the separation of basic compounds

Huang

Wang

et al. 2016

J. Sep. Science

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A novel silica-based stationary phase with branched octadecyl groups was prepared by the sequential employment of the Michael addition reaction and photoinduced thiol-yne click chemistry with 3-aminopropyl-functionalized silica microspheres as the initial material. The resulting stationary phase denoted as SiO2 -N(C18)4 was characterized by elemental analysis, FTIR spectroscopy and Raman spectroscopy, demonstrating the existence of branched octadecyl groups in silica microspheres. The separations of benzene homologous compounds, acid compounds and amine analogues were conducted, demonstrating mixed-mode separation mechanism on SiO2 -N(C18)4 . Baseline separation of basic drugs mixture was acquired with the mobile phase of acetonitrile/H2 O (5%, v/v). SiO2 -N(C18)4 was further applied to separate Corydalis yanhusuo Wang water extracts, and more baseline separation peaks were obtained for SiO2 -N(C18)4 than those on Atlantis dC18 column. It can be expected that this new silica-based stationary phase will exhibit great potential in the analysis of basic compounds.

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“…H sm and S sm are the enthalpy and entropy change associated with the transfer of solute from one phase to another, R the universal gas constant, φ the phase ratio, and T is the absolute temperature [20,21].…”

Section: Thermodynamics Of Retentionmentioning

confidence: 99%

Effect of system variables involved in packed column supercritical fluid chromatography of stavudine taken as model analyte using response surface methodology along with study of thermodynamic parameters

Kaul

Agrawal

Paradkar

et al. 2007

Journal of Pharmaceutical and Biomedical Analysis

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Effect of temperature on precision of retention measurements in liquid chromatography

Cited by 56 publications

References 31 publications

Influence of Temperature on the Enantioselectivity of Koga Tetraamines on Amylose Chiral Stationary Phases

Influence of Temperature on the Enantioselectivity of Koga Tetraamines on Amylose Chiral Stationary Phases

Synthesis of a stationary phase based on silica modified with branched octadecyl groups by Michael addition and photoinduced thiol-yne click chemistry for the separation of basic compounds

Effect of system variables involved in packed column supercritical fluid chromatography of stavudine taken as model analyte using response surface methodology along with study of thermodynamic parameters

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