Effect of Temperature Stress on Antioxidant Defenses in <i>Brassica oleracea</i>

Soengas, Pilar; Rodríguez, Víctor M.; Velasco, Pablo; Cartea, María Elena

doi:10.1021/acsomega.8b00242

Cited by 80 publications

(45 citation statements)

References 29 publications

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“…In photosynthesis, ribulose 1,5-bisphosphate carboxylase/oxidase (rubisco) is a key enzyme involved in the xation of CO2 [38]. Rubisco is involved in various physiological responses such as plant growth, photorespiration, glucose metabolism, heat tolerance, low-temperature stress, and salt stress [39][40][41][42][43]. Many studies have shown that the large rubisco subunit is easily degraded under adverse conditions.…”

Section: Discussionmentioning

confidence: 99%

Proteome-wide and lysine crotonylation profiling reveals the importance of crotonylation in chrysanthemum (Dendranthema grandiforum) under low-temperature

Lin

Bai

et al. 2020

Preprint

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Background: Low-temperature severely affects the growth and development of chrysanthemum which is one kind of ornamental plant well-known and widely used in the world. Lysine crotonylation is a recently identified post-translational modification (PTM) with multiple cellular functions. However, lysine crotonylation under low-temperature stress has not been studied. Results: Proteome-wide and lysine crotonylation of chrysanthemum at low temperature was analyzed using TMT labeling, sensitive immuno-precipitation, and high-resolution LC-MS/MS. The results showed that 2017 crotonylation sites were identified in 1199 proteins. Treatment at 4 °C for 24 h and −4 °C for 4 h resulted in 393 upregulated proteins and 500 downregulated proteins (1.2-fold threshold and P < 0.05). Analysis of biological information showed that lysine crotonylation was involved in photosynthesis, ribosomes, and antioxidant systems. The crotonylated proteins and motifs in chrysanthemum were compared with other plants to obtain orthologous proteins and conserved motifs. To further understand how lysine crotonylation at K136 affected APX (ascorbate eroxidase), we performed a site-directed mutation at K136 in APX. Site-directed crotonylation showed that lysine decrotonylation at K136 reduced APX activity, and lysine complete crotonylation at K136 increased APX activity. Conclusion: In summary, our study comparatively analyzed proteome-wide and crotonylation in chrysanthemum under low-temperature stress and provided insights into the mechanisms of crotonylation in positively regulated APX activity to reduce the oxidative damage caused by low-temperature stress. These data provided an important basis for studying crotonylation to regulate antioxidant enzyme activity in response to low-temperature stress and a new research ideas for chilling-tolerance and freezing-tolerance chrysanthemum molecular breeding.

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Section: Discussionmentioning

confidence: 99%

Proteome-wide and lysine crotonylation profiling reveals the importance of crotonylation in chrysanthemum (Dendranthema grandiforum) under low-temperature

Lin

Bai

et al. 2020

Preprint

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“…Heat stress limits plant growth, leaf development, photosynthesis activity, and yield on Brassica (Rodríguez et al 2015;Soengas et al 2018). Moreover, Rurek et al (2018) found that cauliflower closed stomata during heat stress, resulted in moderate photosynthetic with only minor respiratory damage.…”

Section: Discussionmentioning

confidence: 99%

Adaptation of broccoli (Brassica oleracea var. italica L.) to high and low altitudes in Bali, Indonesia

et al. 2020

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Abstract. Astarini IA, Defiani MR, Suriani NL, Griffiths PD, Stefanova K, Siddique KHM. 2020. Adaptation of broccoli (Brassica oleracea var. italica L.) to high and low altitudes in Bali, Indonesia. Biodiversitas 21: 5263-5269. Broccoli is an important vegetable worldwide, with expanding markets and opportunities in Asia. In Indonesia, there is demand from high-end hotels, restaurants, and export markets, but the local supply is low quality and low yielding. Crown cuts are typically small and misshapen as the varieties are grown are not adapted to local environments. This study targeted new broccoli varieties to identify those best adapted to environmental stresses in Bali, Indonesia, using two-site replicated field experiments. Experiments were undertaken in two regions in Bali (Bedugul and Tabanan) using 13 commercial varieties that included heat-sensitive varieties and others identified as heat-tolerant in trials on the East Coast of the USA. The trials evaluated the varieties for crown initiation, days to maturity, crown size at harvest, crown diameter, and overall performance. The study demonstrated that all 13 varieties could be used for broccoli production in the Bedugul region (1200 m a.s.l. altitude), with Castle Dome being the best performer due to its earlier maturity and large high-quality crown. The varieties Bay Meadows, Belstar, Imperial, and Sarasota could be used in the Tabanan region.

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Section: Response Mechanism Of Lysine Crotonylation and Chrysanthemummentioning

confidence: 99%

Proteome-wide and lysine crotonylation profiling reveals the importance of crotonylation in chrysanthemum (Dendranthema grandiforum) under low-temperature

Lin

Bai

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Low-temperature severely affects the growth and development of chrysanthemum which is one kind of ornamental plant well-known and widely used in the world. Lysine crotonylation is a recently identified post-translational modification (PTM) with multiple cellular functions. However, lysine crotonylation under low-temperature stress has not been studied. Results: Proteome-wide and lysine crotonylation of chrysanthemum at low-temperature was analyzed using TMT (Tandem Mass Tag) labeling, sensitive immuno-precipitation, and high-resolution LC-MS/MS. The results showed that 2017 crotonylation sites were identified in 1199 proteins. Treatment at 4 °C for 24 h and −4 °C for 4 h resulted in 393 upregulated proteins and 500 downregulated proteins (1.2-fold threshold and P < 0.05). Analysis of biological information showed that lysine crotonylation was involved in photosynthesis, ribosomes, and antioxidant systems. The crotonylated proteins and motifs in chrysanthemum were compared with other plants to obtain orthologous proteins and conserved motifs. To further understand how lysine crotonylation at K136 affected APX (ascorbate peroxidase), we performed a site-directed mutation at K136 in APX. Site-directed crotonylation showed that lysine decrotonylation at K136 reduced APX activity, and lysine complete crotonylation at K136 increased APX activity.Conclusion: In summary, our study comparatively analyzed proteome-wide and crotonylation in chrysanthemum under low-temperature stress and provided insights into the mechanisms of crotonylation in positively regulated APX activity to reduce the oxidative damage caused by low-temperature stress. These data provided an important basis for studying crotonylation to regulate antioxidant enzyme activity in response to low-temperature stress and a new research ideas for chilling-tolerance and freezing-tolerance chrysanthemum molecular breeding.

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Effect of Temperature Stress on Antioxidant Defenses in Brassica oleracea

Cited by 80 publications

References 29 publications

Proteome-wide and lysine crotonylation profiling reveals the importance of crotonylation in chrysanthemum (Dendranthema grandiforum) under low-temperature

Proteome-wide and lysine crotonylation profiling reveals the importance of crotonylation in chrysanthemum (Dendranthema grandiforum) under low-temperature

Adaptation of broccoli (Brassica oleracea var. italica L.) to high and low altitudes in Bali, Indonesia

Proteome-wide and lysine crotonylation profiling reveals the importance of crotonylation in chrysanthemum (Dendranthema grandiforum) under low-temperature

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