Effect of the absence of lipoxygenase isoenzymes on the storage characteristics of rice grains

Zhang, Ying; Zhang, Yu; Lu, Yixuan; Yu, Wang; She, Dehong; Song, Maoyong; Wu, Yuejin

doi:10.1016/j.jspr.2005.11.004

Cited by 42 publications

(26 citation statements)

References 15 publications

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“…Based on these information, it is reasonable for us to propose that lipid peroxidation could be one of the main causes of wheat seed aging during storage and inactivated LOXs could extend the seed longevity via alleviating the damage of cell membranes and cellular proteins. Such proposition was also supported by other previous researches (Wilson and McDonald 1986;McDonald 1999;Zhang et al 2007;Huang et al 2014). …”

Section: Discussionsupporting

confidence: 87%

Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

et al. 2015

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In higher plants, lipoxygenases (LOXs) catalyze the oxidation of polyunsaturated fatty acids and can accumulate to relatively high levels in the seeds. However, high LOX activity has often been found associated with harmful effects on the quality attributes and the longevity of harvested seeds. Here, we report the development and characterization of three homozygous T 5 transgenic lines (designated as LOX RNAi -4, 7 and 9, respectively) with LOX activity specifically reduced in the grains of common wheat. An RNAi hairpin directed under an endosperm-specific promoter was introduced into the genome of the elite wheat variety Longchun 23 using particle bombardment. Each transgenic line (LOX RNAi -4, 7 or 9) derived from separate integration event, which carried more than 20 copies of the transgene. The three LOX RNAi lines and wild-type (WT) Longchun 23 showed a similar level of LOX transcripts in the flag leaves. However, relative to WT control, LOX RNAi -4, 7 and 9 exhibited drastic decreases in the level of LOX transcripts during grain development compared with WT control. The LOX activity levels in the harvested grains of the three transgenic lines were significantly lower than that of WT control under either normal conditions or artificial aging (AA, stored at 40°C and 85 % humidity for 6 or 9 days) treatment. The whiteness values of the flour samples from LOX RNAi -4, 7 and 9 were generally and significantly higher than those of WT control regardless of the grains were stored under normal or AA conditions. Furthermore, the germination rates of LOX RNAi -4, 7 and 9 were substantially higher than those of WT control under AA treatment. Our data demonstrate that grainspecific reduction in LOX activity by RNAi may represent a generally applicable approach for improving the quality traits and longevity of crop seeds.Keywords Lipoxygenase Á RNA interference Á Germination rate Á Whiteness Á Wheat Zhenying Dong and Bo Feng have contributed equally to this work.Electronic supplementary material The online version of this article

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Section: Discussionsupporting

confidence: 87%

Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

et al. 2015

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“…To substantiate the storage stability of carotenoids in the transgenic LOX-RNAi seeds, both transgenic and b-carotene-enriched transgenic SKBR-244 rice seeds were aged artificially at 45°C and 85 % RH for 14 days as reported in earlier reports (Li et al 2007;Zhang et al 2007). In general, LOX activity is increased due to storage (artificial aging treatment) (Suzuki et al 1999), which is evident from the increment of LOX activity in SKBR golden rice seeds as compared to LOX-RNAi seeds, which showed Analysis of LOX attenuated transgenic rice seeds # 10-4-2, # 14-10-4 and # 15-5-7 with respect to SKBR-244 after accelerated aging treatment at 45°C and relative humidity at 85 % for 14 days.…”

Section: Discussionmentioning

confidence: 99%

Down-regulation of lipoxygenase gene reduces degradation of carotenoids of golden rice during storage

Gayen

Ali

Sarkar

et al. 2015

Planta

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Down-regulation of lipoxygenase enzyme activity reduces degradation of carotenoids of bio-fortified rice seeds which would be an effective tool to reduce huge post-harvest and economic losses of bio-fortified rice seeds during storage. Bio-fortified provitamin A-enriched rice line (golden rice) expressing higher amounts of β-carotene in the rice endosperm provides vitamin A for human health. However, it is already reported that degradation of carotenoids during storage is a major problem. The gene responsible for degradation of carotenoids during storage has remained largely unexplored till now. In our previous study, it has been shown that r9-LOX1 gene is responsible for rice seed quality deterioration. In the present study, we attempted to investigate if r9-LOX1 gene has any role in degradation of carotenoids in rice seeds during storage. To establish our hypothesis, the endogenous lipoxygenase (LOX) activity of high-carotenoid golden indica rice seed was silenced by RNAi technology using aleurone layer and embryo-specific Oleosin-18 promoter. To check the storage stability, LOX enzyme down-regulated high-carotenoid T3 transgenic rice seeds were subjected to artificial aging treatment. The results obtained from biochemical assays (MDA, ROS) also indicated that after artificial aging, the deterioration of LOX-RNAi lines was considerably lower compared to β-carotene-enriched transgenic rice which had higher LOX activity in comparison to LOX-RNAi lines. Furthermore, it was also observed by HPLC analysis that down-regulation of LOX gene activity decreases co-oxidation of β-carotene in LOX-RNAi golden rice seeds as compared to the β-carotene-enriched transgenic rice, after artificial aging treatment. Therefore, our study substantially establishes and verifies that LOX is a key enzyme for catalyzing co-oxidation of β-carotene and has a significant role in deterioration of β-carotene levels in the carotenoid-enriched golden rice.

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“…Suzuki reported that LOX-3 influenced rice seed storability [13][14][15][16]. In recent years, Wu observed that LOX-1 and LOX-2 were involved in rice storability as well [17]. In this paper, Wanjian 2090, a rice variety lacking LOX-1 and LOX-2, was irradiated by low energy ion beam, and 127 mutation offspring with same field traits and significantly different activity levels of LOX-1 or LOX-2 were screened.…”

Section: Introductionmentioning

confidence: 97%

Mutation of rice (Oryza sativa L.) LOX-1/2 near-isogenic lines with ion beam implantation and study of their storability

Song

Zhang

et al. 2007

Nuclear Instruments and Methods in Physics Research Section B:

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Effect of the absence of lipoxygenase isoenzymes on the storage characteristics of rice grains

Cited by 42 publications

References 15 publications

Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

Down-regulation of lipoxygenase gene reduces degradation of carotenoids of golden rice during storage

Mutation of rice (Oryza sativa L.) LOX-1/2 near-isogenic lines with ion beam implantation and study of their storability

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