“Effect of the addition of different catalase concentrations to a TRIS-egg yolk extender on quality and in vitro fertilization rate of frozen-thawed bull sperm’’

Arslan, Halil Ozancan; Herrera, Carolina; Malama, Eleni; Siuda, Mathias; Leiding, C.; Bollwein, Heinrich

doi:10.1016/j.cryobiol.2019.10.200

Cited by 29 publications

(19 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…While human spermatozoa also contain an aromatic amino acid oxidase, it is not bound and becomes rapidly lost from cells if their plasma membrane integrity has been compromised [72]. One of the obvious solutions to this amino acid oxidase problem in domestic animals has been to incorporate catalase into the sperm cryopreservation medium [73].…”

Section: Cryostoragementioning

confidence: 99%

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

2020

View full text Add to dashboard Cite

This article addresses the importance of oxidative processes in both the generation of functional gametes and the aetiology of defective sperm function. Functionally, sperm capacitation is recognized as a redox-regulated process, wherein a low level of reactive oxygen species (ROS) generation is intimately involved in driving such events as the stimulation of tyrosine phosphorylation, the facilitation of cholesterol efflux and the promotion of cAMP generation. However, the continuous generation of ROS ultimately creates problems for spermatozoa because their unique physical architecture and unusual biochemical composition means that they are vulnerable to oxidative stress. As a consequence, they are heavily dependent on the antioxidant protection afforded by the fluids in the male and female reproductive tracts and, during the precarious process of insemination, seminal plasma. If this antioxidant protection should be compromised for any reason, then the spermatozoa experience pathological oxidative damage. In addition, situations may prevail that cause the spermatozoa to become exposed to high levels of ROS emanating either from other cells in the immediate vicinity (particularly neutrophils) or from the spermatozoa themselves. The environmental and lifestyle factors that promote ROS generation by the spermatozoa are reviewed in this article, as are the techniques that might be used in a diagnostic context to identify patients whose reproductive capacity is under oxidative threat. Understanding the strengths and weaknesses of ROS-monitoring methodologies is critical if we are to effectively identify those patients for whom treatment with antioxidants might be considered a rational management strategy.

show abstract

Section: Cryostoragementioning

confidence: 99%

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

2020

View full text Add to dashboard Cite

show abstract

“…Although laparoscopic insemination in sheep allows the use of frozen‐thawed semen, the development of better extenders is needed for enhancing pregnancy 18 . To achieve convincing fertilization rates, a high percentage of motile and viable frozen‐thawed sperm cells is vital in comparison with fresh spermatozoa 19 …”

Section: Introductionmentioning

confidence: 99%

“…Several studies have deepened to improve sperm quality 5,6,10,13,19‐21 . Cryoprotectants, which have high cell membrane permeability, low molecular weight and minimal toxicity, are used to conserve spermatozoa during cryodamage 7 .…”

Section: Introductionmentioning

confidence: 99%

Combination of fetuin and trehalose in presence of low glycerol has beneficial effects on freeze‐thawed ram spermatozoa

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background Freeze‐thawing process negatively affects ram spermatozoa in terms of sperm quality, DNA integrity and antioxidant defence system. Thus, antioxidant supplementation of spermatozoa during freeze‐thawing is suggested to improve sperm parameters. Objectives The aim of this study was to determine the effects of fetuin and trehalose added into ram semen extender on sperm parameters, antioxidant parameters, antioxidant‐related gene expressions and DNA integrity during the freeze‐thawing process, in low glycerol concentration. Methods Semen samples collected from six mature rams were pooled and splitted into equal aliquots and diluted with a tris‐based extender containing different concentrations of glycerol (G5; %5 and G3; %3), fetuin (F; 2.5, 5 and 15 mg/mL) and trehalose (60 mm) as eight groups (G5F0, G5F2.5, G5F5, G5F15, G3F0, G3F2.5, G3F5 and G3F15). Results G3F5 group resulted in the highest motility, mitochondrial activity and viability and the lowest DNA fragmentation and DNA damage (p < 0.05). Also, G3F0 displayed considerably more cryoprotective effect compared with G5F0 group (p < 0.05) in terms of motility, mitochondrial activity and viability rates. Lipid peroxidation levels decreased in G5F5 group compared with G5F0 group (p < 0.05). The levels of total glutathione increased in G3F2.5 group (p < 0.05) in comparison with the G5F0 group. NQO1 gene levels were upregulated approximately twofold in G5F5, G5F15, G3F2.5, G3F5 and G3F15 groups compared with G5F0 group (p < 0.05). The levels of GCLC gene were approximately twofold higher in G3F0, G3F2.5, G3F5 and G3F15 groups compared with G5F0 group (p < 0.05). GSTP1 gene levels were significantly higher with different levels in all treatment groups except for G5F2.5 and G3F0 groups in comparison with G5F0 group (p < 0.05). Conclusions Co‐supplementation of tris‐based extender having low glycerol (3%) with trehalose and fetuin to enhance the quality of ram spermatozoa after freeze‐thawing process is recommended.

show abstract

“…The imbalance between ROS synthesis and the amounts of antioxidants causes oxidative stress. The presence of oxidative stress damages lipids, proteins and DNA [7] [8]. It has been reported that ROS were involved in the toxicity of organophosphate insecticides (OPIs) [9] and pyrethroid insecticides [7] [10].…”

Section: Introductionmentioning

confidence: 99%

Hepato-Preventive Effects of Hydroethanolic Leaves Extract of Persea americana Mill. (Lauraceae) “Avocado” against Antouka Super®Induced Damage in Male Japanese Quail (Coturnix coturnix Japonica)

Herman¹,

Hervé²,

Jemima³

et al. 2021

OJVM

View full text Add to dashboard Cite

The present study was undertaken to evaluate the protective effects of Hydroethanolic leaves extract of Persea americana (HEPA) against Antouka Su-per® (AS) induced hepatotoxicity in male Japanese quail. In total, 40 immature male Japanese quails aged 28 days were used and divided equally into 5 groups. The groups were designed as the control group (received only a 10 ml/kg of distilled water) and the AS group (75 mg/kg b.w). Other three groups received AS (75 mg of AS/kg b.w) plus HEPA (50, 100, and 200 mg/kg b.w/day respectively) by the oral route. After 60 days of the experiment, the crushed liver was performed to obtain homogenate. The protective effects of HEPA on the biochemical parameters, oxidative stress biomarkers and histology changes in the liver were evaluated. The results indicated that AS treatment caused significant alterations in the clinical signs and behavior. It induces the increase in the content of Urea, Creatinine, Protein, AST and ALT in liver tissues and serum. The activities of enzymatic oxidative stress markers such as Superoxide Dismutase (SOD); Catalase (CAT) and Total Peroxidase (POD) also showed significant perturbations in AS-treated quails.

show abstract

“Effect of the addition of different catalase concentrations to a TRIS-egg yolk extender on quality and in vitro fertilization rate of frozen-thawed bull sperm’’

Cited by 29 publications

References 61 publications

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

Combination of fetuin and trehalose in presence of low glycerol has beneficial effects on freeze‐thawed ram spermatozoa

Hepato-Preventive Effects of Hydroethanolic Leaves Extract of <i>Persea americana</i> Mill. (Lauraceae) “Avocado” against Antouka Super<sup>®</sup>Induced Damage in Male Japanese Quail (<i>Coturnix coturnix</i> Japonica)

Contact Info

Product

Resources

About

“Effect of the addition of different catalase concentrations to a TRIS-egg yolk extender on quality and in vitro fertilization rate of frozen-thawed bull sperm’’

Cited by 29 publications

References 61 publications

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

Combination of fetuin and trehalose in presence of low glycerol has beneficial effects on freeze‐thawed ram spermatozoa

Hepato-Preventive Effects of Hydroethanolic Leaves Extract of &lt;i&gt;Persea americana&lt;/i&gt; Mill. (Lauraceae) “Avocado” against Antouka Super&lt;sup&gt;&#174;&lt;/sup&gt;Induced Damage in Male Japanese Quail (&lt;i&gt;Coturnix coturnix&lt;/i&gt; Japonica)

Contact Info

Product

Resources

About

Hepato-Preventive Effects of Hydroethanolic Leaves Extract of <i>Persea americana</i> Mill. (Lauraceae) “Avocado” against Antouka Super<sup>®</sup>Induced Damage in Male Japanese Quail (<i>Coturnix coturnix</i> Japonica)