Effect of The Addition of Natural and Lyophilized Hen’s Egg Yolk, Egg Yolk Plasma and LDL to Semen Extender on the Freezability and DNA Integrity of Arab Stallion Spermatozo

El-Badry, Diya A.; Anwar, Abeer; Rawash, Z M; Scholkamy, T. H.

doi:10.21608/zvjz.2015.29355

Cited by 3 publications

(3 citation statements)

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“…Despite this, after artificial insemination with frozen goat sperm, the fertility rate is lower than intended, stressing the need to improve the sperm quality. Supplementing antioxidant compounds to semen extenders before cryopreservation is one strategy for preventing oxidative stress [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Ethanolic Purslane (Portulaca oleracea) Leaf Extract Improves the Quality of Cryopreserved Goat Semen

et al. 2023

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Portulaca oleracea contains a wide range of chemical constituents, including flavonoids, alkaloids, polysaccharides, fatty acids, terpenoids, sterols, proteins, vitamins, and minerals. These chemical constituents are linked to the plant’s diverse pharmacological properties, such as antimicrobial and antioxidant activities. Therefore, this study is aimed at evaluating the effect of adding two concentrations (50 or 100 μg/mL) of Portulaca oleracea leaf ethanolic extracts in a native form (POLE) or nanoformulation (POLENF) on the antioxidant status of sperm medium to improve goat sperm freezability. Semen was evaluated postequilibration (5°C for 2 h), postthawing, and 2 h postincubation (37°C and 5% CO2). Phytochemical analysis showed the presence of 30 compounds in POLE, including phenolic acids and flavonoids and their glucosides. Total flavonoids were higher in POLE than in POLENF. Both extracts showed strong free radical scavenging capacity by the 2,2-diphenyl-1-picryl-hydrazyl-hydrate assay, which was higher for POLENF than POLE, whereas the antioxidant activity by the ferric reducing antioxidant power assay had no differences. In comparison to free extender, the addition of POLE (100 μg) or POLENF (50 and 100 μg) significantly improved sperm motility and viability postequilibration and postincubation and antioxidant status of sperm medium postthawing. All POLE and POLENF concentrations significantly improved motility, viability, membrane integrity, and apoptosis of spermatozoa postthawing. Enzyme activities of AST, ALT, and LDH in sperm medium postthawing were not affected by treatments. In comparing different types and concentrations of POLE with free extender, the addition of POLENF (100 μg/mL) to goat semen extender gave the most valuable results as an effective free radical scavenger and antiapoptotic during cryopreservation.

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Section: Introductionmentioning

confidence: 99%

Ethanolic Purslane (Portulaca oleracea) Leaf Extract Improves the Quality of Cryopreserved Goat Semen

et al. 2023

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“…Ashrafi et al (2019) reported that chicken egg yolk was used as semen extender at the end of the 1930s. Over the years, numerous studies showed that it has been successfully used as a component of semen extenders for mammals, mainly bulls (Ashrafi et al, 2019; Ngoula et al, 2014), horses (El‐Badry et al, 2015), rams (Alcay et al, 2015; Marco‐Jimenez et al, 2004), boars (Selige et al, 2021) and dogs (Belala et al, 2019). Recent study has reported the first, great success in the preservation of maned wolf semen after the use of Tris‐yolk extender (Teodoro et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Effect of freeze‐dried quail egg white and yolk addition to semen extender on viability of rooster sperm stored for 6 h at 4°C

Łukaszewicz

Jerysz

Kowalczyk

2023

Reprod Domestic Animals

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Rooster sperm loses its fertilizing capacity very quickly shortly after ejaculation (Łukaszewicz, 1988); therefore, it is necessary to ensure the appropriate environment allowing for an elongation of its storage outside the bird's body. Semen dilution is a very important initial step of in vitro processing, storage and preservation for efficient artificial insemination (Smith et al., 2018). Numerous studies indicate a significant influence of diluents and storage time on sperm morphology and motility parameters in chicken (Donoghue

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“…The decline in sperm viability and fertility related to cryopreservation motivated the development of new studies to address the preservation of semen in refrigerated form for the buffalo species (Dhami and Sahni, 1994;Adeel et al, 2009;Akhter et al, 2011b ;Singh et al, 2012;Almeida et al, 2015;Almeida et al, 2016a;Almeida et al, 2017and Almeida et al, 2020a, 2020b2021), also low-density lipoproteins (LDLs) have been the subject of research for chilled (Almeida et al, 2016b) and frozen buffalo semen (Akhter et al, 2011a;Brito, 2014;El-Badry et al, 2015and Almeida et al, 2020a, 2020b2021). However, there is a paucity of information on the use of LDLs for chilled buffalo semen.…”

Section: Introductionmentioning

confidence: 99%

Viability of Spermatozoa Refrigerated at 5 °c for 120 Hours: Efficiency of Different Extenders in the Maintenance of Buffer Semen for Use in Ftai

Almeida¹,

Resende²

2023

JASR

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The objective of the present study was to verify the effectiveness of different extenders used for cooling buffalo semen in relation to maintenance of sperm longevity throughout processing. Seminal samples of 11 Murrah bulls aged 5 to 6 years were preserved at 5 °C for 120 hours in a passive refrigeration system (refrigerated counter) using TRIS extenders with 10% LDL (replacing egg yolk), Tolera D® (soy lecithin replacing egg yolk) and (Botu-Bov®). These samples were subjectively evaluated at T0 (post-collection), T1, T24, T48, T72, T96 and T120 hours post-dilution and refrigeration through computerized sperm movement analysis (CASA), plasmatic membrane integrity (Hyposmotic Test). Each male had his ejaculate analyzed for the physical and morphological characteristics of the semen, which were divided into three aliquots for each of the extenders. The values obtained were submitted to the Friedman Test (P<0.05). From the results achieved, it can be concluded that there was a better preservation of the samples kept under refrigeration with the Botu-Bov® extender at 5 °C/120 hours, providing greater protection against injuries and integrity of the spermatic membrane, when compared to the others. Thus, the Botu-Bov® extender can be an alternative for the use of refrigerated buffalo semen in AI and TAI for up to 48 hours.

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Effect of The Addition of Natural and Lyophilized Hen’s Egg Yolk, Egg Yolk Plasma and LDL to Semen Extender on the Freezability and DNA Integrity of Arab Stallion Spermatozo

Cited by 3 publications

References 0 publications

Ethanolic Purslane (Portulaca oleracea) Leaf Extract Improves the Quality of Cryopreserved Goat Semen

Ethanolic Purslane (Portulaca oleracea) Leaf Extract Improves the Quality of Cryopreserved Goat Semen

Effect of freeze‐dried quail egg white and yolk addition to semen extender on viability of rooster sperm stored for 6 h at 4°C

Viability of Spermatozoa Refrigerated at 5 °c for 120 Hours: Efficiency of Different Extenders in the Maintenance of Buffer Semen for Use in Ftai

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