Effect of the addition of β-mannanase on the performance, metabolizable energy, amino acid digestibility coefficients, and immune functions of broilers fed different nutritional levels

Abstract

Three experiments were conducted to evaluate the effect of β-mannanase (BM) supplementation on the performance, metabolizable energy, amino acid digestibility, and immune function of broilers. A total of 1,600 broilers were randomly distributed in a 4 × 2 factorial arrangement (4 nutritional levels × 0 or 500 g/ton BM), with 10 replicates and 20 broilers per pen. The same design was used in the energy and digestibility experiments with 8 and 6 replicates, respectively, and 6 broilers per pen. The nutritional levels (NL) were formulated to meet the nutritional requirements of broilers (NL1); reductions of 100 kcal metabolizable energy (NL2); 3% of the total amino acids (NL3); and 100 kcal metabolizable energy and 3% total amino acids (NL4) from NL1. The serum immunoglobulin (Ig) concentration was determined in two broilers per pen, and these broilers were slaughtered to determine the relative weight of spleen, thymus, and bursa of Fabricius. Throughout the experiment, the lower nutritional levels reduced (P < 0.05) body weight gain (BWG) and increased (P < 0.05) feed conversion (FCR) for the NL4 treatment. The BM increased (P < 0.05) the BWG values and improved (P < 0.05) the FCR of the broilers. The apparent metabolizable energy corrected for nitrogen balance (AMEn) values were reduced (P < 0.05) for NL2 and NL3. The BM increased (P < 0.05) the AMEn values and reduced (P < 0.05) the excreted nitrogen. NL3 and NL4 reduced (P < 0.05) the true ileal digestibility coefficients (TIDc) of the amino acids cystine and glycine, and BM increased (P < 0.05) the TIDc for all amino acids. The addition of BM reduced (P < 0.05) the relative weights of the spleen and bursa. NL2 increased (P < 0.05) the Ig values, whereas BM reduced (P < 0.05) the serum IgA, IgG, and IgM values of the broilers. This study indicates that using suboptimal nutrient levels leads to losses in production parameters, whereas BM-supplemented diets were effective in improving performance, energy values, and TIDc levels of amino acids and immune response of broilers.

Effect of Using Different Guar Meal Levels and Β– Mannanase Supplementation on Productive Performance of Golden Montazah Laying Hens

“…Decreasing thymus weight percent by βmannanase supplementation agree with Zou, et al (2006). Moreover the relative decrease in thymus weight percent by βmannanase supplementation was about 27%, this result was in agreement with Ferreira et al (2016).…”

Effects of dietary β-mannanase supplementation on the additivity of true metabolizable energy values for broiler diets

“…These results may indicate that dietary β-mannanase has little effects on true energy metabolizability in ingredients and diets fed to broiler chickens. However, previous experiments reported that dietary β-mannanase increased apparent metabolizability of nutrients [ 9 , 11 ] and energy [ 20 ] in diets fed to broiler chickens. The reason for this discrepancy is not clear, but it may be related to the effect of dietary β-mannanase on endogenous energy losses because we measured true metabolizable energy but previous experiments measured apparent metabolizable energy as affected by dietary β-mannanase.…”

Effect of β-Mannanase (Hemicell®) on Growth Performance and Immunity of Japanese quail

“…Feeding of prebiotics (beta mannans) was found to increase immune status and enhance the macrophage activity along with T-helper cell activities (Shohani et al, 2013) and increased most of the relative immune organ weights and significantly increased the concentration of serum IgM. Moreover, βmannanase (Hemicell) supplementation can attenuate the immune response of broilers caused by the reduced production of immunoglobulins (IgA) (Li et al, 2010 andFerreira et al, 2016) and leukocytes (Mehri et al, 2010) because of the mannans present in the feed stimulate the immune system. Li et al (2010) and Ferreira et al (2016) found that supplementing diets with β-mannanase reduced the weights of immunity related organs such as the thymus and bursa of Fabricius.…”

“…Moreover, βmannanase (Hemicell) supplementation can attenuate the immune response of broilers caused by the reduced production of immunoglobulins (IgA) (Li et al, 2010 andFerreira et al, 2016) and leukocytes (Mehri et al, 2010) because of the mannans present in the feed stimulate the immune system. Li et al (2010) and Ferreira et al (2016) found that supplementing diets with β-mannanase reduced the weights of immunity related organs such as the thymus and bursa of Fabricius. It has been reported that prebiotics as mannan oligosaccharides from yeast cell wall works by providing specific binding sites to enteric pathogens, thus reduces their chances to attach to the intestinal tract and these oligosaccharides being not digested by the endogenous enzymes of the bird pass through the gut with the pathogens attached thus producing a cleaning up effect (Zopf and Roth, 1996).…”

“…There are advantages of using Hemicell in diets because it increased body weight gain and improved food conversion ratio throughout the entire experimental period. In addition, increased AM En and immune system attenuation were observed by (Ferreira et al, 2016); thus, Hemicell supplementation is an important tool for formulating diets to improve performance parameters. Animals with activated immune systems can redirect nutrients acquired in the diet to produce immune response-related molecules, which reduce the nutrients required for maintenance and growth and are directly reflected in /performance losses.…”