Abstract:In order to enhance the handling ability and mechanical properties of chelate-setting β-tricalcium phosphate (β-TCP) cement, various polysaccharides, such as sodium alginate, sodium dextran sulfate, sodium chondroitin sulfate, and chitosan (Chito GL and W10), were added to mixing solutions for cement preparation. When mixing solutions containing the above additives were used, the handling ability of all the cement pastes was improved compared with the case of pure water without polysaccharide. Among the examin… Show more
“…However, the number of cells increased with prolonging cell culture period. 18 As such, the present results suggested that although dissolution of chitosan affected the cytotoxicity to the cells initially, the viability of the cells was improved via prolonged cell culturing. Figure 5.Biocompatibility assay showing cell proliferation co-cultured with the cement: (a) 1000-IP6-HAp(+0) and ultrapure water (P/L = 1/0.35 [g·cm −3 ]) and (b) 1000-IP6-HAp(+0) and 10 mass% GL (P/L = 1/1.1).…”
Section: Resultssupporting
confidence: 50%
“…However, the number of cells increased with prolonging cell culture period. 18 As such, the present results suggested that although dissolution of chitosan affected the cytotoxicity to the cells initially, the viability of the cells was improved via prolonged cell culturing.…”
Section: In Vitro and In Vivo Evaluation Of Ip6-hap Cementssupporting
An injectable chelate-setting hydroxyapatite cement (IP6-HAp), formed by chelate-bonding capability of inositol phosphate (IP6), was developed. The effects of ball-milling duration of starting HAp powder and IP6 concentration on the material properties such as injectability and mechanical strength of the cement were examined. The cement powder was prepared by ball-milling the as-synthesized HAp powder for 5 min using ZrO beads with a diameter of 10 mm, followed by another 60 min with ZrO beads with a diameter of 2 mm, and thereafter surface-modified with 5000 ppm of IP6 solution. Injectable cement was then fabricated with this HAp powder and 2.5 mass% chitosan as a mixing solution, with a setting time of 36.3 ± 4.7 min and a compressive strength of 19.0 ± 2.1 MPa. The IP6-HAp cements prepared with chitosan showed favorable biocompatibility in vitro using an osteoblast cell model, and osteoconductivity in vivo using a pig tibia model.
“…However, the number of cells increased with prolonging cell culture period. 18 As such, the present results suggested that although dissolution of chitosan affected the cytotoxicity to the cells initially, the viability of the cells was improved via prolonged cell culturing. Figure 5.Biocompatibility assay showing cell proliferation co-cultured with the cement: (a) 1000-IP6-HAp(+0) and ultrapure water (P/L = 1/0.35 [g·cm −3 ]) and (b) 1000-IP6-HAp(+0) and 10 mass% GL (P/L = 1/1.1).…”
Section: Resultssupporting
confidence: 50%
“…However, the number of cells increased with prolonging cell culture period. 18 As such, the present results suggested that although dissolution of chitosan affected the cytotoxicity to the cells initially, the viability of the cells was improved via prolonged cell culturing.…”
Section: In Vitro and In Vivo Evaluation Of Ip6-hap Cementssupporting
An injectable chelate-setting hydroxyapatite cement (IP6-HAp), formed by chelate-bonding capability of inositol phosphate (IP6), was developed. The effects of ball-milling duration of starting HAp powder and IP6 concentration on the material properties such as injectability and mechanical strength of the cement were examined. The cement powder was prepared by ball-milling the as-synthesized HAp powder for 5 min using ZrO beads with a diameter of 10 mm, followed by another 60 min with ZrO beads with a diameter of 2 mm, and thereafter surface-modified with 5000 ppm of IP6 solution. Injectable cement was then fabricated with this HAp powder and 2.5 mass% chitosan as a mixing solution, with a setting time of 36.3 ± 4.7 min and a compressive strength of 19.0 ± 2.1 MPa. The IP6-HAp cements prepared with chitosan showed favorable biocompatibility in vitro using an osteoblast cell model, and osteoconductivity in vivo using a pig tibia model.
“…In addition, this cement showed excellent biocompatibility both in vitro and in vivo [11]. Meanwhile, we have previously developed biodegradable cement consisting of a single-phase -TCP based on the chelate-setting mechanism of IP6, which was biodegradable and osteoconductive in vivo using rabbit models [14][15][16].…”
Biodegradableα-tricalcium phosphate (α-TCP) cement based on the chelate-setting mechanism of inositol phosphate (IP6) was developed. This paper examined the effect of the milling time ofα-TCP powder on the material properties of the cement. In addition, biocompatibility of the result cementin vitrousing osteoblasts andin vivousing rabbit models will be studied as well. Theα-TCP powders were ballmilled using ZrO2beads in pure water for various durations up to 270 minutes, with a single-phaseα-TCP obtained at ballmilling for 120 minutes. The resulting cement was mostly composed ofα-TCP phase, and the compressive strength of the cement was8.5±1.1 MPa, which suggested that the cements set with keeping the crystallite phase of starting cement powder. The cell-culture test indicated that the resulting cements were biocompatible materials.In vivostudies showed that the newly formed bones increased with milling time at a slight distance from the cement specimens and grew mature at 24 weeks, and the surface of the cement was resorbed by tartrate-resistant acid phosphatase-(TRAP-)positive osteoclast-like cells until 24 weeks of implantation. The presentα-TCP cement is promising for application as a novel paste-like artificial bone with biodegradability and osteoconductivity.
“…In addition, we used aqueous chitosan solution as the mixing solution to enhance handling ability and mechanical properties of the IP6-HAp cement mixed with the ball-milled α-TCP powders according to a previous report 14 . Konishi et al successfully enhanced handling ability and mechanical properties of the β-tricalcium phosphate (β-TCP) chelate-setting cements by adding chitosan as a cohesion promoter to mixing solutions for cement preparation.…”
We have previously developed a chelate-setting calcium-phosphate cement (CPC) consisting of hydroxyapatite (HAp) powders surface-modified with inositol hexaphosphate (IP6). In the present study, mechanically ball-milled α-tricalcium phosphate (α-TCP) powders were added into the IP6-HAp cement in order to enhance its anti-washout capability and mechanical properties. After optimization of the ball-milling conditions, the cement specimens mixed with various amounts of the prepared α-TCP powders were fabricated. Examinations of their mechanical properties revealed significant increase of compressive strength (CS) with the addition of the α-TCP powders. Further, it was demonstrated that the specimens of the IP6-HAp powders / the α-TCP powders mixing ratios of 20/80 and 30/70 [g/g] exhibited comparatively high anti-washout capability. In conclusion, these cements are promising chelate-setting CPCs with enhanced anti-washout capability and mechanical properties.
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