Effect of the anticodon loop size of yeast alanyl tRNA on its biological activity

Parameters for the molecular recognition of transfer RNAs

“…This is consistent with genetic studies which show that substitutions in the anticodon loop and stem, and of the position 49-65 base pair in the TUC stem, do not interfere with acceptance of alanine (Hou & Schimmel, 1988). It is of interest to note that the anticodon of yeast tRNAA1* is also not required for recognition by the yeast enzyme (Jin et al, 1987). 4: Suhstrate levels of alanine tRNA synthetase do not significantly aminoacylate A3-U70, G3-C70, or U3-G70 tRN AAla at pH 7.5, 37 °C.…”

Synthetic RNA molecules as substrates for enzymes that act on tRNAs and tRNA-like molecules

“…Synthetic strategies based in part on RNA ligase have also been used in the synthesis of tRNAs from their constituent nucleotides. 61 An alternative strategy for generating RNA sequence variants is based on the use of RNA polymerases from prokaryotic and eukaryotic sources.…”

Debao Wang, the founder of nucleic acid biology and industry in People’s Republic of China

“…They proposed that the synthetic yeast alanine tRNA has the same chemical composition (containing 9 modified nucleotides of 7 different species) and structure as the natural yeast alanine tRNA, making it biologically active (Wang, 1983a). A comparison of the charging activities of yeast alanyl tRNA and reconstituted natural yeast tRNA revealed that the anticodon loop of yeast alanyl tRNA was not involved in the interaction between alanyl-tRNA synthetase from rat liver and yeast alanyl-tRNA (Jin, 1987). This work has won the first prize of science and technology achievements of the Chinese Academy of Sciences, the first prize of the National Natural Science Foundation, and the Tan Kah Kee Award in Life Sciences.…”