Effect of the arginine on some blood and biochemical parameters of Awassi Ewes

Abstract

This study was carried out in The Ruminant Research Station (Abu Ghraib)/ Department of Animal Resources Research/ Office of Agricultural Research/ Ministry of Agriculture during the period from July 6, 2017 to Feb 3, 2018. Twenty one non pregnant Awassi Turkish ewes were used in the experiment. The age of the animals ranged between 2-5 years old with a weight ranged between 45-70kg. Befor insert CIDR, The animals were examined with ultrasonography to be sure that they were not pregnant. These ewes were divided into three equal groups of 7 ewes/ treatment. They were fed naturally on basal diet. The first group were not treated (T1) which serve Control group, and injected with 5-10 ml of normal saline in intramuscular. Ewes of Second group were Treated (T2) by injected with a 155 µmol/kg arginine I.V. The Third group were Treated (T3) by injected with 155µmol/kg arginine intramuscular. All Treated ewes were inseminated by rams for 7 days. The inseminate rams were revolved among folds to get rid of their effects on ewes, Each inseminated ram was given a number. Blood samples were taken from jugular vein on the 1st and 2nd days of arginine injection and then insert CIDR and on the 3rd, 5th, 7th, 10th,13th ,and 16th days of this treatment to measure the change in the levels of a blood and chemical properties during arginine treatment. The results of a lab. analysis during the treatment of amino acid arginine showed no significant differences between the treated group with arginine as compared to the control group in the percentages of white blood cells, but there were a differences in the number of Neutrophils in the group of intravenous injection, the group of intramuscular injection, (75.14, 75.00), respectively as compared with control group (71.0). Lymphocytes showed no significant differences between parameters under study as well as for Eosinophil. The differences were observed between the coefficients and the study periods. The period (4) was significantly superior (P<0.05) in the arginine treated as Compared with the control group, (20.42 ± 2.61) and the percentage of intravenous injection (17.28±1.98), respectively compared with a control group (19.00 ± 2.51), as for Basophil, there was a gradual decrease in all study groups. The results showed that the value of cholesterol a significant superiority (P<0.05) in the treated group with arginine in ewes serum as compared with the control group. It was noticed that the group of intravenous injection of arginine significantly increase the group of intramuscular injection of arginine (P<0.05) (6, 8) in blood cholesterol level. The periods (1, 2, 3, 4, 5, and 7) of injection of amino acid arginine showed no significant differences between them. The glucose concentration level, showed no significant differences between the arginine amino acid treatment group and the control group and at the different. It was concluded from this study that treatment with amino acid arginine did not affect characteristics of the blood, and its chemical properties.

Sonographic changes during Postpartum Uterine Involution in Sahel Goats (Capra hircus)

“…It is also in agreement with the report of Greyling (2000) and Enginler et al (2017) whom stated that complete uterine involution in slaughtered animals is 28 days, the mean involution period in single and twin saturated does to be 27.10±0.43 and 28.29±0.54 days respectively, and complete uterine involution in goats occurred within 28 days postpartum. The current study is also in close agreement with previous reports (Baru et al, 1983;Tian and Noakes, 1991;Sanchez et al, 2002;Ababneh and Degefa, 2005;Takayama et al, 2010;Zongo et al, 2014;Medan and El-Daek, 2015) but is in contrast with the report of others (Rubianes et al, 1996;Zdunczyk et al, 2004;Fernandes et al, 2013;Fasulkov, 2014;Ahmed et al, 2016;Souhayla et al, 2016) who observed the end of uterine involution after 30 days. Shorter days postpartum (16 days) period was also reported by Sanchez et al (2002)…”

Determination of the effects of oxytocin and carazolol on uterine involution by pulsed-wave Doppler ultrasonography in Kıvırcık ewes

“…In a study, the effect of prostaglandins (PGF2α) and oxytocin on postpartum uterine involution of Awassi ewes were evaluated (3) which the postpartum gravid horn diameter was found as 6.50±0.45 and 6.45±0.25 in the PGF2α and oxytocin groups, respectively, on the 7 th day after parturition. On the 21 st day of pp, Ahmed et al (3) found the diameter to be 2.92±0.43 and 3.45±0.13 in the PGF2α and oxytocin groups, respectively. The pp gravid horn diameter decreased more rapidly in the current study than in Ahmed et al's (3) study.…”

“…There are very few reports on postpartum uterine involution in small ruminants, and most of them focus on postmortem evaluations (11,17,22); moreover, the previous studies on the postpartum uterus were mostly performed only by B-mode USG (1,3,18,38). This study was designed to investigate, by pulsed-wave Doppler sonography, the involution of the sheep uterus in the postpartum period following the delivery of medications that contract the uterus.…”

Immunopathological and Comparative Study of Concanavalin- A and Streptococcus lyophilized Antigen in Immunized Rats

“…Experimental rats were euthanized and a full necropsy was performed to detect any abnormalities and gross changes in liver and spleen that dissected after that 1c.m³ from organs were taken then fixed in 10% neutral buffered formalin for (2-3) days, then routinely processed in histokinette (ATP-1000\HESTION), tissue sections were embedded in paraffin (Eman et al, 2016) after that paraffin blocks with implanted tissue slices were sectioned at 5 µm thickness by microtome) (Yidi) (Ahmed and Bushra, 2013), and staining by routine hematoxylin and eosin stain then examined under light microscope(Olympus) according to (Steven and Bancroft, 1997).…”