Effect of the Combination of Gelam Honey and Ginger on Oxidative Stress and Metabolic Profile in Streptozotocin-Induced Diabetic Sprague-Dawley Rats

Abstract

Diabetic complications occur as a result of increased reactive oxygen species (ROS) due to long term hyperglycaemia. Honey and ginger have been shown to exhibit antioxidant activity which can scavenge ROS. The main aim of this study was to evaluate the antioxidant and antidiabetic effects of gelam honey, ginger, and their combination. Sprague-Dawley rats were divided into 2 major groups which consisted of diabetic and nondiabetic rats. Diabetes was induced with streptozotocin intramuscularly (55 mg/kg body weight). Each group was further divided into 4 smaller groups according to the supplements administered: distilled water, honey (2 g/kg body weight), ginger (60 mg/kg body weight), and honey + ginger. Body weight and glucose levels were recorded weekly, while blood from the orbital sinus was obtained after 3 weeks of supplementation for the estimation of metabolic profile: glucose, triglyceride (TG), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH): oxidized glutathione (GSSG), and malondialdehyde (MDA). The combination of gelam honey and ginger did not show hypoglycaemic potential; however, the combination treatment reduced significantly (P < 0.05) SOD and CAT activities as well as MDA level, while GSH level and GSH/GSSG ratio were significantly elevated (P < 0.05) in STZ-induced diabetic rats compared to diabetic control rats.

Beneficial effects of ginger Zingiber officinale Roscoe on obesity and metabolic syndrome: a review

“…Of these two types, the latter is the more prevalent form and generally receives more attention. To date, a number of studies have demonstrated that ginger extract can exert beneficial effects on serum glucose and tissue glycogen content in alloxan-induced 39 and streptozotocin-induced diabetic animal models [40][41][42][44][45][46][47] through enhancing the peripheral utilization of glucose, regulating the activities of glycolytic enzymes, and limiting gluconeogenic formation. 45 Ginger extract could increase the activities of glycolytic enzymes, which drives the metabolic degradation of glucose to form pyruvate.…”

“…Recent studies indicated that ginger and its bioactive components exerted a therapeutic protective effect in diabetes by regulating oxidative stress markers and decreasing activities of antioxidant enzymes. 25,43,44,46,47 Furthermore, it was demonstrated that 6-gingerol could protect pancreatic ␤ cells from oxidative stress by reducing the artificial d-glyceraldehyde-induced increase in the intracellular reactive oxygen species (ROS) level in RIN-5F cells. 48 On the other hand, it is generally accepted that inflammation is usually associated with the pathogenesis of T2DM.…”

Does honey have any salutary effect against streptozotocin - induced diabetes in rats?

“…However, Bahrami and his colleagues reported that honey had no significant difference in blood glucose concentration of diabetic patients with type 2 diabetes. When honey used to treat diabetic rats, they showed no improvement in blood glucose concentration [27]. …”

Aktivitas Antioksidan dan Sifat Fisikokimia Madu Temulawak (Curcuma zanthorrhiza roxb) yang Ditambah Ekstrak Jahe (Zingiber officinale Rosc)

“…Kombinasi madu dan rempah-rempah seperti temulawak dan jahe diduga dapat menaikkan aktivitas antioksidannya. Kombinasi madu gelam dan jahe dapat menurunkan kadar malonaldehid sebagai hasil oksidasi lipid pada tikus diabetes (Sani et al, 2014). Hasil penelitian Septiana et al (2017) menunjukkan bahwa minuman temulawak mempunyai aktivitas antioksidan lebih besar dibandingkan minuman kunyit, jahe maupun minuman beras kencur.…”