Effect of the combination of NAA and BA on callus induction from hypocotyl explants in black cumin (Nigella sativa L.)

Abstract

Black cumin (Nigella sativa L.) seeds contain the main bioactive compound thymoquinone, which is beneficial for health. Callus cell culture techniques are an alternative method for the production of plant bioactive compounds. Therefore, it was necessary to develop a method for producing callus in vitro. This study evaluated the effect of a combination of NAA and BA growth regulators on MS medium on in vitro callus induction using black cumin hypocotyl explants. There were two variables used in the nature of the study: NAA (1 and 2 mg/L) and BA (1, 1,5, 2, 5 and 3 mg/L). All hypocotyl explants were able to induce callus on all medium used. The callus appeared between 8-11 days. A combination of NAA and BA influenced black cumin hypocotyl callus formation and development. With NAA 1 mg/L added and BA 1.5 mg/L added, MS medium was able to induce best hypocotyl explants to shape and develop callus with an average callus weight of 0.262 g/Explant. However, the time of callus emergence was not significantly affected by the combination of NAA and BA growth regulators.

Improvement of Culture Conditions and Plant Growth Regulators for In Vitro Callus Induction and Plant Regeneration in Paeonia lactiflora Pall.

“…This observation is consistent with the findings of Guo et al (2021), who reported a positive effect of NAA on callus induction in Abies koreana [39]. However, it is worth noting that the previous study did not find a significant effect of NAA concentration on the formation time of black cumin callus [40]. Many studies have found that PIC plays an important role in the induction of callus [41][42][43][44].…”

Induction of Axillary Bud Swelling of Hevea brasiliensis to Regenerate Plants through Somatic Embryogenesis and Analysis of Genetic Stability

“…Based on these data, basic MH medium was selected for callus induction, and different combinations and concentrations of 2,4-D, NAA, KT, and sucrose were checked for the optimum combination of callus induction. For callus induction, 2,4-D and NAA are required, and kinetin stimulates the callus proliferation [38,39]. The present study indicated that MH medium supplemented with KT (1.5 mg/L), 2,4-D (1.5 mg/L), NAA (1.5 mg/L), and sucrose 70 g/L was the most suitable combination for inducing a callus from AB swelling.…”

In vitro Induced Mutagenesis for Early Blight (<i>Alternaria solani</i>) Resistance in Potato (<i>Solanum tuberosum</i> L.)

“…2.4-D for callus induction has proven its vitality for almost all types of plants [20]. For explants which failed to show good callus induction response in the presence of 2, 4-D, addition of small amounts of cytokinin proved to be effective [21][22]. Specific receptor sites for auxins are present on cells which probably are some auxin binding protein [23] that activates the plasma membrane H + ATPase.…”