Effect of the culture medium and biotic stimulation on taxane production in Taxus globosa Schltdl in vitro cultures

Tapia, Nadia; Zamilpa, Alejandro; Bonfill, Mercedes; Ventura, Elsa; Cruz-Vega, Delia Elva; Villar, Alma Del; Cruz-Sosa, Francisco; Osuna, Lidia

doi:10.1007/s11738-013-1380-0

Cited by 19 publications

(16 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Surprisingly, following 4 successive subcultures, MIIS and MIL failed to provide a suitable feeding condition for the calli, since a substantial reduction of both calli weight and viability occurred and also the incidence of necrosis increased significantly. The same results were also recorded by the previous studies (Bruňáková et al 2004;Tapia et al 2013). Such or similar observations may generally attribute to the deficiency of some additives including amino acid supplies, ascorbic acid and/or citric acid (as an antioxidant) to stimulate callus growth and proliferation.…”

Section: Callus Maintenancesupporting

confidence: 87%

“…With regard to taxane extraction from the calli, the methodology of (Tapia et al 2013) was employed, with some minor modifications: Concisely, for each medium, *100 mg DW samples of powdered freeze-dried biomass were first subjected into 4 mL of hexane and left at room temperature. After 12 h, each sample was separately centrifuged at 5000 rpm for 20 min and the supernatant was discarded.…”

Section: Elicitation and Taxanes Isolationmentioning

confidence: 99%

“…The results of Induction index, however, were somewhat in keeping with the observations of Mihaljević Fig. 2 The hierarchical structure to generate a ranking strategy of refined maintenance media for a leaf-and b stem-derived calli of Taxus baccata Table 5 Calculation of five criteria weights on the basis of 10 pairwise comparisons 1 1/2 1/7 1/4 1/5 0.045349 2 1 1/6 1/5 1/5 0.059018 7 6 1 5 2 0.468433 4 5 1/5 1 1/2 5 5 1/2 2 1 Correa et al (2006) (96 % from fresh stems) and Tapia et al (2013) (100 and 80 % from needles and fresh stems), albeit in the last study the induction of calli from stems was lower than that of leaves. Anyway, taking the second parameter Calli weight index (CWI; as the average calli weight for both fresh and dry weight) into account, a clear discrimination pattern was also recognized, which made it consequently possible to detach the most appropriate media from the weakest ones: the same as the previous section, MIIS with the quantity of 0.872 ± 0.069 was again the superior, and the cultures MIL, MIS and MIIS had lower CWI (Table 6).…”

Section: Callus Inductionmentioning

confidence: 99%

“…To minimize such considerable drawbacks, plant cell and tissue culture has been utilized widely, as a more promising and cost-effective alternative system (reviewed by (Malik et al 2011;Ikeuchi et al 2013;Us-Camas et al 2014). Irrespective of its remarkable ability to improve the yield of various plant natural products in vitro (Wickremesinhe and Arteca 1993;Bruňáková et al 2004;Kajani et al 2012;Shukor et al 2013;Sokkar et al 2013;Tapia et al 2013;Lodha et al 2014), owing to different known and unknown factors, for each plant species this technique needs to be optimized either for callus induction or continuous callus growth, making the accurate selection process of the best media complicated. This phenomenon would be more difficult, particularly, if one needs to make a correct decision in terms of some criteria with different priorities.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Modified AHP-based decision-making model toward accurate selection of eligible maintenance media for production of taxanes in Taxus baccata callus culture

et al. 2015

View full text Add to dashboard Cite

Attempts were made here to apply a modified analytic hierarchy process (AHP) approach based on refinement assay of dominated alternatives in monitoring the most reliable callus maintenance media (supplemented with L-glutamine and Casamino acid) of Taxus baccata callus cultures in terms of five criteria. Generally, regarding stem-derived calli, 6 out of 18 maintenance media were nominated as non-dominated alternatives, and following AHP ranking test Casamino acid-based media (i.e., A12, A15 and A19) were overall nominated as the premiere. Taking leaf-derived calli into account, only L-glutaminebased media in an ascending order of A8, A4, A6, A5, A9 and A3 were introduced as non-dominated alternatives. Such results connote that L-glutamine-based feeding appears to generate more significant results either for continuous calli growth or taxanes production. In contrast, regarding the second explant, stem, both amino acid supplies had fairly equal worth. Our findings, overall, demonstrate promising applications of the proposed AHP method regarding accurate selection of the best callus maintenance cultures of T. baccata for production of different taxanes including paclitaxel, Baccatin III and 10-deacetylbaccatin III. Similarly, this statistical approach could be also applicable for other crops, for instance, for accurate selection of the best callus cultures/media and consequently production improvement of a given plant secondary metabolite/product.

show abstract

Section: Callus Maintenancesupporting

confidence: 87%

Section: Elicitation and Taxanes Isolationmentioning

confidence: 99%

Section: Callus Inductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Modified AHP-based decision-making model toward accurate selection of eligible maintenance media for production of taxanes in Taxus baccata callus culture

et al. 2015

View full text Add to dashboard Cite

show abstract

“…A wavelength range of 200-400 nm was utilized by setting the analysis at 220 nm. A commercial sample of Taxol ® was used as a reference (Tapia et al, 2013).…”

Section: Acute Toxicity Modelmentioning

confidence: 99%

Taxus globosa Schltdl. (Mexican yew) and Taxus baccata L. (European yew): intra and interspecies analysis of taxol content and biological activity according to different sources

Osuna-Torres

Garcia-Martí²,

Ventura-Zapata

et al. 2015

For. syst.

View full text Add to dashboard Cite

Aim of study: Taxus globosa is the only species of Taxus genus that grows in Mexico, and is a tree which is protected by the Mexican Government. This plant shares, with the European species T. baccata, the ability to produce taxanes, such as taxol. The objective of this work was to compare representative samples from two Taxus species: T. globosa Schltdl.("Mexican yew"), and T. baccata L. ("European yew"), by employing different methodological chemical and pharmacological tests.Area of study: Representative samples of Mexican yew were collected from states of Nuevo León, Querétaro, Hidalgo, and Oaxaca. The samples of European yew were taken from a clonal live collection located in the CIEF-Vaersa-Valencia (Spain). Materials and Methods:The aerial parts of all samples were extracted with methanol and subjected to TLC and HPLC analysis. Five extracts were selected for their anti-inflammatory evaluation. The extracts were tested on male ICR mice with ear inflammation induced with TPA, at a dose of 3.2 mg/ear. For T. globosa, an acute toxicity assay in female mice, according to OECD, was also conducted.Main results: Taxol was only detected in the yew trees samples collected in States of Hidalgo and Nuevo León (Mexico). In the inflammatory assay, in all treatments (except for that of MX35) induced slight, but significant, inhibition of inflammation, and only European sample M-9 showed inhibition of edema of >50%. T. globosa in the acute toxicity test causes only mild symptoms associated with a general depression of CNS, without being lethal.Research highlights: The aim of the present study was to perform a chemical analysis through quantification of taxol in the Mexican yew compared to European yew. In this research work was evaluated too for the first time the anti-inflammatory effect from both the Mexican and European yew.Keywords: Acute toxicity; anti-inflammatory activity; European yew; HPLC; Mexican yew. Citation: Osuna-Torres, L., García-Martí, X., Ventura-Zapata, E., López-Upton, J., Zamilpa-Alvarez, A., González-Cortazar, M., Herrera-Ruiz, M., Tapia-Barrera, N. (2015). Taxus

show abstract

Plant Cell and Organ Culture as an Alternative for the Production of Anticancer Compounds

Murthy

Dandin

Sebastian

et al. 2018

Anticancer Plants: Natural Products and Biotechnological Implements

View full text Add to dashboard Cite

Effect of the culture medium and biotic stimulation on taxane production in Taxus globosa Schltdl in vitro cultures

Cited by 19 publications

References 28 publications

Modified AHP-based decision-making model toward accurate selection of eligible maintenance media for production of taxanes in Taxus baccata callus culture

Modified AHP-based decision-making model toward accurate selection of eligible maintenance media for production of taxanes in Taxus baccata callus culture

Taxus globosa Schltdl. (Mexican yew) and Taxus baccata L. (European yew): intra and interspecies analysis of taxol content and biological activity according to different sources

Plant Cell and Organ Culture as an Alternative for the Production of Anticancer Compounds

Contact Info

Product

Resources

About