Effect of the distance between adherent mesenchymal stem cell and the focus of irradiation of femtosecond laser on cell replication capacity

Sakai, Jun; Roldan, Daniel; Ueno, Kosei; Misawa, Hiroaki; Hosokawa, Yoichiroh; Iino, Takanori; Wakitani, Shigeyuki; Takagi, Mutsumi

doi:10.1007/s10616-012-9437-2

Cited by 4 publications

(1 citation statement)

References 13 publications

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“…In recent papers, femtosecond pulse laser-induced shock waves were used to isolate adherent cells as a manipulator and its effectiveness is verified in the field of biology [11][12][13]. In addition, micro bubbles have been observed at the laser-focal point when focusing the laser and generating shock waves.…”

Section: Introductionmentioning

confidence: 99%

Numerical Simulation to Investigate Interactions of Generated Underwater Micro Shock Waves and Micro Bubbles by Focusing Femtosecond Pulse Laser

Ayumu Yamamoto,

Kazuteru Toh,

Masaaki Tamagawa

2023

ARNHT

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The purpose of this study is to elucidate the mechanism of propagation of the laser-induced micro shock waves under condition where the micro bubbles are generated. In this paper, effects of generated micro bubbles on propagation of the laser-induced micro shock waves were investigated by CFD (computational fluid dynamics). Firstly, the two models (1-D model and 1-D spherical symmetric model) were computed for comparison of the peak pressure variation of the shock waves with propagation. As for governing equations for the propagation of the shock waves, continuity equation, Euler’s momentum equation and Tait’s state equation are used. From the computation, it is confirmed that attenuation of pressure of the 1-D spherical symmetric model was earlier than the 1-D model. In addition, the attenuation of the 1-D spherical symmetric model agreed with the laser-induced shock waves obtained experimentally. However, the peak pressure and duration time of the shock wave was not the same as the experimental result. Then, the bubble behavior was included in the computation of the shock wave propagation. As for the bubble behavior, Rayleigh-Plesset equation is used. From this computation, pressure wave was obtained which superposed the pressure of the shock wave on the internal pressure of the bubble. Although the duration time of the pressure wave was close to the experimental result, the value of the pressure was almost the same as atmospheric pressure. It is suggested that there is a possibility that phenomenon other than the bubbles is generated such as plasma when the shock wave is generated by focusing the femtosecond pulse laser

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Section: Introductionmentioning

confidence: 99%

Numerical Simulation to Investigate Interactions of Generated Underwater Micro Shock Waves and Micro Bubbles by Focusing Femtosecond Pulse Laser

Ayumu Yamamoto,

Kazuteru Toh,

Masaaki Tamagawa

2023

ARNHT

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Cell Processing Engineering for Regenerative Medicine

Takagi

2014

Bioreactor Engineering Research and Industrial Applications II

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The cell processing engineering including automatic cell processing and noninvasive cell quality estimation of adherent mammalian cells for regenerative medicine was reviewed. Automatic cell processing necessary for the industrialization of regenerative medicine was introduced. The cell quality such as cell heterogeneity should be noninvasively estimated before transplantation to patient, because cultured cells are usually not homogeneous but heterogeneous and most protocols of regenerative medicine are autologous system. The differentiation level could be estimated by two-dimensional cell morphology analysis using a conventional phase-contrast microscope. The phase-shifting laser microscope (PLM) could determine laser phase shift at all pixel in a view, which is caused by the transmitted laser through cell, and might be more noninvasive and more useful than the atomic force microscope and digital holographic microscope. The noninvasive determination of the laser phase shift of a cell using a PLM was carried out to determine the three-dimensional cell morphology and estimate the cell cycle phase of each adhesive cell and the mean proliferation activity of a cell population. The noninvasive discrimination of cancer cells from normal cells by measuring the phase shift was performed based on the difference in cytoskeleton density. Chemical analysis of the culture supernatant was also useful to estimate the differentiation level of a cell population. A probe beam, an infrared beam, and Raman spectroscopy are useful for diagnosing the viability, apoptosis, and differentiation of each adhesive cell.

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Time-lapse analysis of laser phase shift for noninvasive discrimination of human normal cells and malignant tumor cells

Takagi

Shibaki

2012

Journal of Bioscience and Bioengineering

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Effect of the distance between adherent mesenchymal stem cell and the focus of irradiation of femtosecond laser on cell replication capacity

Cited by 4 publications

References 13 publications

Numerical Simulation to Investigate Interactions of Generated Underwater Micro Shock Waves and Micro Bubbles by Focusing Femtosecond Pulse Laser

Numerical Simulation to Investigate Interactions of Generated Underwater Micro Shock Waves and Micro Bubbles by Focusing Femtosecond Pulse Laser

Cell Processing Engineering for Regenerative Medicine

Time-lapse analysis of laser phase shift for noninvasive discrimination of human normal cells and malignant tumor cells

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