Effect of the EGTA (Ethylene Glycol Tetraacetic Acid) Supplementation in the Freezing Extender on Quality of Cryopreserved Bull Sperm

Posado, R.; Jj, García Pérez; Gómez-Fernández, J.; Tomás, C.; Gómez-Izquierdo, E.; E, de Mercado

doi:10.17352/ijvsr.000017

Cited by 2 publications

(4 citation statements)

References 43 publications

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“…Inclusion of antioxidants into the semen freezing media is beneficial for sperm protection during the freezing/thawing in several species (Affonso et al., 2017; Ashrafi et al., 2011; ChaithraShree et al., 2020; Posado et al., 2017). In this study, following determination of optimal doses of myo‐inositol and melatonin based on the sperm motility, we investigated the protective effect of myo‐inositol and melatonin co‐supplementation on the goat semen cryopreservation.…”

Section: Discussionmentioning

confidence: 99%

“…As presented in Table 1, the percentage of cleavage rate was significantly higher in both individual and combined groups of 5 μM myo-inositol and 1 mM melatonin compared to the control group (p < .05). In addition, the combined group of 5 μM myo-inositol Inclusion of antioxidants into the semen freezing media is beneficial for sperm protection during the freezing/thawing in several species (Affonso et al, 2017;Ashrafi et al, 2011;ChaithraShree et al, 2020;Posado et al, 2017). In this study, following determination of optimal doses of myo-inositol and melatonin based on the sperm motility, we investigated the protective effect of myo-inositol and melatonin cosupplementation on the goat semen cryopreservation.…”

Section: Combined Effects Of Myo-inositol and Melatonin On In Vitro F...mentioning

confidence: 98%

“…However, contradictory results have been reported using various types of antioxidants on improvement of post‐freeze results. The main reasons for this discrepancy can be related to the type of antioxidant, sample size, type of species and evaluation method (Ashrafi et al., 2011; ChaithraShree et al., 2020; Mohammadi et al., 2019; Posado et al., 2017).…”

Section: Introductionmentioning

confidence: 99%

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Synergistic effects of myo‐inositol and melatonin on cryopreservation of goat spermatozoa

Vash

Nadri

Karimi

2022

Reprod Domestic Animals

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Overproduction of reactive oxygen species (ROS) during sperm cryopreservation has a detrimental effect on sperm parameters. Therefore, the use of antioxidants in the sperm freezing extender can reduce ROS destructive effects. In this study, we investigated whether co-supplementation of melatonin and myo-inositol into the semen extender can improve the post-cryopreservation quality of goat spermatozoa. After the freeze-thawing process, sperm motility, viability, plasma membrane and acrosome intact morphology were improved in the combined myo-inositol and melatonin group compared to both individual and the control groups (p < .05). In addition, the mean of sperm ROS, DNA damage and lipid peroxidation were reduced in co-supplementation of myo-inositol and melatonin compared to their individual counterparts (p < .05).Therefore, the synergistic effects of myo-inositol and melatonin on the cryopreserved spermatozoa are highly likely mediated through the reduction in important factors involved in the sperm lipid peroxidation. Finally, we used the cryopreserved spermatozoa for in vitro production of embryos. Results showed that combined group of myo-inositol and melatonin improved the cleavage rate compared to both individual and control groups, although blastocyst rate was improved using both individual and combined groups. In conclusion, co-supplementation of melatonin and myo-inositol is a promising approach for the improvement of goat sperm cryopreservation.

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Section: Discussionmentioning

confidence: 99%

Section: Combined Effects Of Myo-inositol and Melatonin On In Vitro F...mentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Synergistic effects of myo‐inositol and melatonin on cryopreservation of goat spermatozoa

Vash

Nadri

Karimi

2022

Reprod Domestic Animals

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“…The sperm samples from different groups were used for evaluating acrosome reaction and intracellular calcium levels. To find out a sufficient concentration, the different concentrations of L-glutamate (1, 5, and 10 mM) (Berdichevsky et al, 1983), MK-801 (50 and 100 μM) (Spinaci et al, 2017), kainic acid (25, 50 and 100 μM) (Berdichevsky et al, 1983) and EGTA (1, 5 and 10 mM) (Tomas et al, 2015) was used.…”

Section: Preparation and The Treatment Of Mouse Spermatozoamentioning

confidence: 99%

A novel role for involvement of N‐methyl‐D‐aspartate (NMDA) glutamate receptors in sperm acrosome reaction

et al. 2021

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The acrosome reaction is a calcium-dependent process where the binding of the spermatozoa to the oocyte induces the reaction to enable spermatozoa for penetrating several layers surrounding the oocyte (Cuasnicú et al., 2016). In mammals, the binding of sperm receptors to the oocyte zona pellucid (ZP3) activates a G protein, which may involve in activating voltage-gated Ca 2+ channels, leading to the plasma membrane depolarisation. This initial and transient Ca 2+ stimulates phospholipase C δ (PLC δ ), which in turn catabolises phosphatidylinositol bisphosphate (PIP 2 ) for producing second messengers, including inositol three phosphates (IP 3 ) and diacylglycerol (DAG). IP 3 binds to its receptors located in the acrosome and/or redundant nuclear envelope (RNE), releasing stored Ca 2+ into the cytosol. Released Ca 2+ stimulates store-operated channels (SOC), further increases cytosolic Ca 2+ level for triggering the acrosome reaction (Darszon et al., 2011). Such a signalling cascade has been suggested to elevate the concentration of Ca 2+ in the sperm cytosol for inducing acrosome reaction in the presence of the oocyte factors, for instance, ZP3. In contrast, there are no oocyte factors in vitro;

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Effect of the EGTA (Ethylene Glycol Tetraacetic Acid) Supplementation in the Freezing Extender on Quality of Cryopreserved Bull Sperm

Cited by 2 publications

References 43 publications

Synergistic effects of myo‐inositol and melatonin on cryopreservation of goat spermatozoa

Synergistic effects of myo‐inositol and melatonin on cryopreservation of goat spermatozoa

A novel role for involvement of N‐methyl‐D‐aspartate (NMDA) glutamate receptors in sperm acrosome reaction

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