Effect of the Freeze-Drying Process on the Physicochemical and Microbiological Properties of Mexican Kefir Grains

Conde-Islas, Alicia Águeda; Jiménez‐Fernández, Maribel; Cantú-Lozano, Denis; Urrea-García, Galo R.; Luna‐Solano, Guadalupe

doi:10.3390/pr7030127

Cited by 16 publications

(5 citation statements)

References 51 publications

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“…What is worth noticing is the fact that probiotics provided in a form of dietary supplements or within food are not medicinal products. Their influence on an organism and health benefits depend on several factors, including the source of a strain, with spontaneously fermented food being particularly beneficial to the human digestive tract, as well as geographical origins of a strain, supplemented population match, and production process, like food additives, humidity, pH, fermentation, and storage or freeze-drying conditions [ 36 , 106 ].…”

Section: Probiotics As Pharmaceutical Dietary Supplementsmentioning

confidence: 99%

Probiotics: Versatile Bioactive Components in Promoting Human Health

et al. 2020

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The positive impact of probiotic strains on human health has become more evident than ever before. Often delivered through food, dietary products, supplements, and drugs, different legislations for safety and efficacy issues have been prepared. Furthermore, regulatory agencies have addressed various approaches toward these products, whether they authorize claims mentioning a disease’s diagnosis, prevention, or treatment. Due to the diversity of bacteria and yeast strains, strict approaches have been designed to assess for side effects and post-market surveillance. One of the most essential delivery systems of probiotics is within food, due to the great beneficial health effects of this system compared to pharmaceutical products and also due to the increasing importance of food and nutrition. Modern lifestyle or various diseases lead to an imbalance of the intestinal flora. Nonetheless, as the amount of probiotic use needs accurate calculations, different factors should also be taken into consideration. One of the novelties of this review is the presentation of the beneficial effects of the administration of probiotics as a potential adjuvant therapy in COVID-19. Thus, this paper provides an integrative overview of different aspects of probiotics, from human health care applications to safety, quality, and control.

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Section: Probiotics As Pharmaceutical Dietary Supplementsmentioning

confidence: 99%

Probiotics: Versatile Bioactive Components in Promoting Human Health

et al. 2020

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“…Powdered probiotics are convenient for subsequent processing and transportation, and have longer shelf lives compared to those of liquid probiotics [ 2 , 3 , 4 ]. Freeze-drying achieves better survival rates compared to other drying techniques, thus making it an effective technique for the production of probiotic powders [ 5 ]. However, during the freeze-drying process, long-term exposure to extreme environmental conditions could damage the physical properties of cell membranes or the structure of cell-sensitive proteins, thus lowering the survival rates of freeze-dried probiotics [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of Temperature during Freeze-Drying Process on the Viability of Bifidobacterium longum BB68S

et al. 2023

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Maintaining optimum temperature during freeze-drying is crucial to ensuring the viability of strains. In this study, we evaluated the effect of pre-freezing, sublimation and desorption temperatures on the viability of Bifidobacterium longum BB68S (BB68S). Moreover, we examined the water content, water activity, enzyme activities, and scanning electron microscope of BB68S to explore mechanisms underpinning the effect of temperature on viability. Our analyses revealed the highest survival rates of BB68S collected after pre-freezing and sublimation drying at −40 °C (94.9 ± 2.2%) and −10 °C (65.4 ± 3.8%), respectively. Additionally, response surface methodology demonstrated that the optimum conditions for freeze-drying of BB68S were pre-freezing temperature at −45.52 °C and sublimation temperature at −6.58 °C, and the verification test showed that survival rates of BB68S could reach 69.2 ± 3.8%. Most of the vitality loss occurred during the sublimation drying phase. Further studies showed that different sublimation temperatures affected water content and activity, β-galactosidase, lactate dehydrogenase, Na+-K+-ATP and Ca2+-Mg2+-ATP activities. In conclusion, the temperature during freeze-drying, especially sublimation temperature, is a key factor affecting the survival rate of BB68S, and the vitality loss during freeze-drying process might be due to compromised cell membrane integrity and permeability.

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“…The moisture content in a dry food is generally associated with its stability, quality, and composition, which could affect its storage, packaging, and processing. Therefore, the residual moisture in a dry product must be less than 6% [26]. In this research, a residual moisture content in all lemon extract microcapsules of less than 4% was obtained (Table 1).…”

Section: Moisture Content and Water Activity (Aw)mentioning

confidence: 63%

Effect of Extraction Methods and In Vitro Bio-Accessibility of Microencapsulated Lemon Extract

et al. 2022

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The extraction of bioactive compounds from fruits, such as lemon, has gained relevance because these compounds have beneficial properties for health, such as antioxidant and anticancer properties; however, the extraction method can significantly affect these properties. High hydrostatic pressure and ultrasound, as emerging extraction methods, constitute an alternative to conventional extraction, improving extractability and obtaining extracts rich in bioactive compounds. Therefore, lemon extracts (LEs) were obtained by conventional (orbital shaking), ultrasound-assisted, and high-hydrostatic-pressure extraction. Extracts were then microencapsulated with maltodextrin at 10% (M10), 20% (M20), and 30% (M30). The impact of microencapsulation on LEs physicochemical properties, phenolics (TPC), flavonoids (TFC) and relative bio-accessibility (RB) was evaluated. M30 promoted a higher microencapsulation efficiency for TPC and TFC, and a longer time required for microcapsules to dissolve in water, as moisture content, water activity and hygroscopicity decreased. The RBs of TPC and TFC were higher in microcapsules with M30, and lower when conventional extraction was used. The data suggest that microencapsulated LE is promising as it protects the bioactivity of phenolic compounds. In addition, this freeze-dried product can be utilized as a functional ingredient for food or supplement formulations.

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Effect of the Freeze-Drying Process on the Physicochemical and Microbiological Properties of Mexican Kefir Grains

Cited by 16 publications

References 51 publications

Probiotics: Versatile Bioactive Components in Promoting Human Health

Probiotics: Versatile Bioactive Components in Promoting Human Health

Influence of Temperature during Freeze-Drying Process on the Viability of Bifidobacterium longum BB68S

Effect of Extraction Methods and In Vitro Bio-Accessibility of Microencapsulated Lemon Extract

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