Effect of the g.–723G→T Polymorphism in the Bovine Myogenic Factor 5 (Myf5) Gene Promoter Region on Gene Transcript Level in the Longissimus Dorsi Muscle and on Meat Traits of Polish Holstein-Friesian Cattle

Robakowska-Hyżorek, Dagmara; Oprządek, J.; Żelazowska, Beata; Olbromski, Rafał; Zwierzchowski, L.

doi:10.1007/s10528-009-9328-1

Cited by 15 publications

(14 citation statements)

References 37 publications

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“…Previously we showed that in 12-month-old HF bulls the g.-723G>T transition located within the promoter region of the MYF5 gene influenced the gene expression in the LD muscle and was associated with sirloin weight and fat weight in sirloin in carcasses of HF cattle (Robakowska-Hyzorek et al 2010).…”

Section: Discussionmentioning

confidence: 95%

“…TATA box binding protein (TBP) gene was chosen for normalization of expression of MYF6 and MYF5 genes as the most stably expressed in bovine muscle (Robakowska et al 2010). The real-time polymerase chain reactions were performed using the following mix: 0.8 µl of 10µM forward and reverse primers; the Power SYBR ® Green PCR Master Mix (added according to the manufacturer's protocol); 500 ng cDNA used as a template.…”

Section: Methodsmentioning

confidence: 99%

“…Due to their functions, MRFencoding genes are considered candidate markers for meat production in farm animals. Several studies have shown associations between sequence nucleotide polymorphisms in MRF genes and muscle mass and carcass traits in pigs (Liu et al 2008), cattle (Li et al 2004;Bhuiyan et al 2009;Robakowska-Hyzorek et al 2010), and chicken (Yin et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Effects of polymorphism in the bovine PTPRQ gene on the expression of MYF6 and MYF5 genes in skeletal muscle and on meat production traits in beef bulls

Robakowska-Hyżorek¹,

Starzyński²,

Żelazowska³

et al. 2016

CZECH J ANIM SCI

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ABSTRACT:The aim of the study was to characterize nucleotide sequence polymorphisms in the bovine PTPRQ gene, to search for their possible effect on the expression of myogenic factor 6 (MYF6; MRF4) and myogenic factor 5 (MYF5) genes in skeletal muscle and on meat production traits. Three novel SNPs were found in intron 35 of the bovine PTPRQ gene: g.200,451A>G, g.200,467T>C, g.200,480C>T (GenBank Acc. No. NW_001494990.2; counted from translation initiation site). These SNPs are placed very closely to each other (within 29 base pairs). The results showed that genotype influenced the expression of MYF6 and MYF5 genes in longissimus dorsi muscle of Limousine bulls both at the transcript and protein levels. Moreover, an association was found between the PTPRQ genotype and carcass traits in Limousine bulls. These findings suggest that bovine PTPRQ gene may contain regulatory sequences for MRF genes located 24 kb downstream. The results also showed that nucleotide sequence polymorphisms in the PTPRQ gene may influence meat production traits in beef cattle, possibly through the regulation of the MRF genes expression.

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Section: Discussionmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of polymorphism in the bovine PTPRQ gene on the expression of MYF6 and MYF5 genes in skeletal muscle and on meat production traits in beef bulls

Robakowska-Hyżorek¹,

Starzyński²,

Żelazowska³

et al. 2016

CZECH J ANIM SCI

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“…Its upstream region is highly conserved [27]. Robakowska-Hyzorek et al [28] found that mutation g.-723G ? T affected sirloin weight and fat mass of Polish Holstein-Friesian cattle.…”

Section: Discussionmentioning

confidence: 99%

Eight SNPs of the Myf5 gene and diplotypes associated with growth and reproductive traits in Jinghai yellow chicken

Ying

Zhang

et al. 2014

Mol Biol Rep

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The objective of this study was to analyze possible associations between single nucleotide polymorphisms (SNPs) in the Myf5 gene with chicken growth and reproductive traits. SNPs in Myf5 of the Jinghai yellow chicken were detected by the polymerase chain reaction single-strand conformation polymorphism method and the haplotypes were analyzed. Eight SNPs were identified in the exons of Myf5. Nine haplotypes were established in a group of 379 Jinghai yellow chickens. In terms of growth traits, least square analysis showed that haplotype H1H5 had significant effects on weight at weeks 8 and 12 (P < 0.05). Haplotype H2H6 had significant effects on weight at weeks 12 and 14 (P < 0.05). For reproductive traits, H1H5 had higher body weight for the first egg than H1H4 and H2H4 (P < 0.05), and H1H3 (P < 0.01). H1H3 had a poor performance in average egg weight at 300 days. On the other hand, H1H3 had an advantage in egg number at 300 days. The results showed that SNPs of Myf5 have certain effects on growth and reproductive traits in Jinghai yellow chickens, which can be used in marker-assisted selection to accelerate chicken genetic progress.

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“…Moreover, polymorphisms in the porcine MyF-5 gene and its relationship with different meat traits in different pig lines and breeds have also been reported (Venza et al, 2009;Kunhareang et al, 2009;Robakowska-Hyzorek et al, 2010).…”

Section: Snp Marker Associationsmentioning

confidence: 98%

Back fat thickness and meat tenderness are associated with a 526 T→A mutation in the exon 1 promoter region of the MyF-5 gene in Chinese Bos taurus

Ujjan

Liu²,

Ujan³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. Qualitative trait loci (QTL) for growth and meat quality traits in cattle (Bos taurus) have been previously mapped to three chromosome regions, 0 to 30, 55 to 70, and 70 to 80 cM on chromosome 5. We evaluated the allele frequencies and gene-specific single nucleotide polymorphisms (SNPs) of bovine myogenic factor 5 (MyF-5) in the QTL regions and their associations with live weight and meat characteristics in indigenous Chinese cattle breeds. PCR-SSCP methodology showed a T>A mutation at 526 bp. Least square analysis revealed a significant association of this SNP with backfat thickness and meat tenderness (P < 0.05), while no significant association was found with live weight, loin eye height, loin eye area, rib area, or water holding capacity. Association of MyF-5 gene with meat quality traits in cattle crossbreed, respectively. The genotype distributions for these alleles in two of the Chinese cattle breeds (Luxi and Qinchuan) were not in Hardy-Weinberg equilibrium (P < 0.05); while those for the other three breeds (JiaXian red, Nanyang, and XiaNan) were in agreement with Hardy-Weinberg equilibrium (P > 0.05). The genotypic frequencies among all five cattle breeds showed moderate diversity (0.25 < polymorphism information content < 0.5). Based on our findings, we suggest that the MyF-5 gene influences back fat thickness and meat tenderness in Chinese Bos taurus. This SNP could be useful for markerassisted selection for meat quality traits in these cattle.

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Effect of the g.–723G→T Polymorphism in the Bovine Myogenic Factor 5 (Myf5) Gene Promoter Region on Gene Transcript Level in the Longissimus Dorsi Muscle and on Meat Traits of Polish Holstein-Friesian Cattle

Cited by 15 publications

References 37 publications

Effects of polymorphism in the bovine PTPRQ gene on the expression of MYF6 and MYF5 genes in skeletal muscle and on meat production traits in beef bulls

Effects of polymorphism in the bovine PTPRQ gene on the expression of MYF6 and MYF5 genes in skeletal muscle and on meat production traits in beef bulls

Eight SNPs of the Myf5 gene and diplotypes associated with growth and reproductive traits in Jinghai yellow chicken

Back fat thickness and meat tenderness are associated with a 526 T→A mutation in the exon 1 promoter region of the MyF-5 gene in Chinese Bos taurus

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