Effect of the harvest period on the structure and anti-allergic activity of Porphyra haitanensis polysaccharides

Abstract

With the delayed harvest period, the molecular weight and sulfate content of Porphyra haitanensis polysaccharides decreased and their anti-allergic activity increased.

Recent progress in Porphyra haitanensis polysaccharides: Extraction, purification, structural insights, and their impact on gastrointestinal health and oxidative stress management

“… Fraction Extraction method Purification process Yield of polysaccharides Monosaccharide composition Molecular weight Backbone or linkages Ref. PHP0.5–1-UF, PHP1.0–1-UF Thermal extraction, 80 °C hot water at ratio of 20:1 (w/v) for 2 h DEAE-Sepharose FF Sephacryl S-400 HR 37.1%, 48.6% – 6.68 × 10 6 Da 1.14 × 10 6 Da – ( Chen, Xu, et al, 2021 ) PHPs Thermal extraction, 80 °C hot water material to water ratio of 0.04， for 3 h – 20.48% galactose, glucose, and fucose (76.2:2.1:1) 6.3 × 10 5 Da – ( Dong et al, 2020 ) PHPs Thermal extraction, hot water DEAE-cellulose 52 – 3,6-anhydrogalactose – – ( Liu et al, 2017 ) PHP1 PHP2 PHP3 Thermal extraction, 90 °C hot water for 4 h DEAE-cellulose 52 – galactose, glucose, xylose, mannose, fructose, and glucuronic acid (98.10:0.54:0.19:0.36:0.15:0.66 94.27:3.95:0.28:0.46:0.26:0.78 96.91:1.66:0.19:0.54:0.17:0.53) 5.67 × 10 5 Da 4.14 × 10 5 Da 3.23 × 10 5 Da – ( Wang, Ye, Wang, & Fu, 2022 ) PHPs Thermal extraction, 80 °C hot water at ratio of 20:1 ( w / v ) for 2 h – – – – – ( Wang, Zhong, et al, 2023 ) PHP Thermal extraction, 90 °C hot water at ratio of 30:1 (w/v) for 2 h – 4.10% galactose (94.85%), glucose (3.18%), mannose (1.97%) from 2.623 × 10 5 to 2.308 × 10 4 Da 1 → 3 and 1 → 4 linked Galp ( Xu et al, 2019 ...…”

Therapy and mechanism of natural polysaccharides for atopic dermatitis treatment

“…However, excessively high or low sulfate content may not further improve the anti-inflammatory activity of polysaccharides, indicating that an optimal sulfate content is crucial for their effectiveness [117]. For example, a study on the polysaccharides obtained from Porphyra haitanensis at different growth periods found that polysaccharide samples from early harvests had higher sulfate content and molecular weights than those from late harvests, but the polysaccharides had lower anti-allergic activity than the latter [118].…”