Effect of the molecular weight of chitosan on its antifungal activity against Candida spp. in planktonic cells and biofilm

Garcia, Lana Glerieide Silva; Guedes, Gláucia Morgana de Melo; Silva, Maria Lucilene Queiroz da; Castelo-Branco, Débora de Souza Collares Maia; Sidrim, José Júlio Costa; Cordeiro, Rossana de Aguiar; Rocha, Marcos Fábio Gadelha; Vieira, Rodrigo Silveira; Brilhante, Raimunda Sâmia Nogueira

doi:10.1016/j.carbpol.2018.04.091

Cited by 71 publications

(30 citation statements)

References 45 publications

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“…Contrary to the present study, the biofilm formation of Candida spp. was decreased by about 70% in adhesion and 80% in the mature phase by CS solution [48]. The authors described CS as a promising anti-candidiasis agent [45].…”

Section: Discussionmentioning

confidence: 99%

Antifungal and Surface Properties of Chitosan-Salts Modified PMMA Denture Base Material

et al. 2020

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Chitosan (CS) and its derivatives show antimicrobial properties. This is of interest in preventing and treating denture stomatitis, which can be caused by fungi. Therefore, the aim of this study was the development of a novel antifungal denture base material by modifying polymethyl methacrylate (PMMA) with CS-salt and characterizing its antifungal and surface properties in vitro. For this purpose, the antifungal effect of chitosan-hydrochloride (CS-HCl) or chitosan-glutamate (CS-G) as solutions in different concentrations was determined. To obtain modified PMMA resin specimens, the CS-salts were added to the PMMA before polymerization. The roughness of these specimens was measured by contact profilometry. For the evaluation of the antifungal properties of the CS-salt modified resins, a C. albicans biofilm assay on the specimens was performed. As solutions, both the CS-G and CS-HCl-salt had an antifungal effect and inhibited C. albicans growth in a dose-dependent manner. In contrast, CS-salt modified PMMA resins showed no significant reduced C. albicans biofilm formation. Furthermore, the addition of CS-salts to PMMA significantly increased the surface roughness of the specimens. This study shows that despite the antifungal effect of CS-salts in solution, a modification of PMMA resin with these CS-salts does not improve the antifungal properties of PMMA denture base material.

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Section: Discussionmentioning

confidence: 99%

Antifungal and Surface Properties of Chitosan-Salts Modified PMMA Denture Base Material

et al. 2020

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“…and Cryptococcus spp. pathogenic yeasts [34,36,42,50,51,52]. As for filamentous fungi, chitosan inhibits C. albicans growth under the same nutritional status (carbon) as in human blood (glycemia).…”

Section: Chitosan As Antimicrobial Agentmentioning

confidence: 99%

Molecular Mechanisms of Chitosan Interactions with Fungi and Plants

López-Moya

Suarez-Fernandez

López-Llorca

2019

IJMS

199

108

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Chitosan is a versatile compound with multiple biotechnological applications. This polymer inhibits clinically important human fungal pathogens under the same carbon and nitrogen status as in blood. Chitosan permeabilises their high-fluidity plasma membrane and increases production of intracellular oxygen species (ROS). Conversely, chitosan is compatible with mammalian cell lines as well as with biocontrol fungi (BCF). BCF resistant to chitosan have low-fluidity membranes and high glucan/chitin ratios in their cell walls. Recent studies illustrate molecular and physiological basis of chitosan-root interactions. Chitosan induces auxin accumulation in Arabidopsis roots. This polymer causes overexpression of tryptophan-dependent auxin biosynthesis pathway. It also blocks auxin translocation in roots. Chitosan is a plant defense modulator. Endophytes and fungal pathogens evade plant immunity converting chitin into chitosan. LysM effectors shield chitin and protect fungal cell walls from plant chitinases. These enzymes together with fungal chitin deacetylases, chitosanases and effectors play determinant roles during fungal colonization of plants. This review describes chitosan mode of action (cell and gene targets) in fungi and plants. This knowledge will help to develop chitosan for agrobiotechnological and medical applications.

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“…Candida spp.Nesta pesquisa foram utilizadas cepas padrão de C. albicans (ATCC 10231), C. parapsilosis (ATCC 22019), C. krusei (ATCC 6258) e uma cepa de isolado clínico de C. tropicalis. O teste de atividade antifúngica seguiu a metodologia descrita pelaGarcia et al (2018). As cepas de Candida spp.…”

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“…podem ainda formar biofilme, dificultando o tratamento das infecções. Biofilme é uma comunidade de células aderentes cujas propriedades diferem daquelas das células planctônicas (LOPEZ; VLAMAKIS;KOLTER, 2010;GARCIA et al, 2018). Os institutos nacionais de saúde estimam que os biofilmes são responsáveis por mais de 80% das infecções microbianas nos Estados Unidos (FOX; NOBILE, 2012).…”

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Processo De Territorialização Em Saúde: Estratégias E Possibilidades

Almeida¹,

Silveira²,

Nicola³

et al. 2019

As Ciências Da Vida Frente Ao Contexto Contemporâneo 2

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Ciência. 2. Ciências da vida -Pesquisa -Brasil. I. Pereira, Denise. II. Série.CDD 570.9 Elaborado por Maurício Amormino Júnior -CRB6/2422 O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores.2019 Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.

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Effect of the molecular weight of chitosan on its antifungal activity against Candida spp. in planktonic cells and biofilm

Cited by 71 publications

References 45 publications

Antifungal and Surface Properties of Chitosan-Salts Modified PMMA Denture Base Material

Antifungal and Surface Properties of Chitosan-Salts Modified PMMA Denture Base Material

Molecular Mechanisms of Chitosan Interactions with Fungi and Plants

Processo De Territorialização Em Saúde: Estratégias E Possibilidades

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