Effect of the polar headgroup of phospholipids on their interaction with actin

Bihan, T. Le; Pelletier, Diana; Tancrède, Pierre; Heppell, Benoit; Chauvet, Jean-Paul; Gicquaud, Claude

doi:10.1016/j.jcis.2005.02.090

Cited by 12 publications

(9 citation statements)

References 36 publications

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“…Therefore, it is very unlikely to desorb the L-arg from the DDP monolayers only by compression. Moreover, at a certain temperature, the desorption should take place at almost the same surface pressure [34]. Since the π-A isotherms at 15 • C reach to 0.50 nm 2 /molecule at different surface pressures depending on the subphase concentration of L-arg, the possibility of the desorption of L-arg from the monolayers can be ruled out.…”

Section: Interactions Of L-arg With Ddp Monolayersmentioning

confidence: 99%

Interactions of l-arginine with Langmuir monolayers of di-n-dodecyl hydrogen phosphate at the air–water interface

Hossain

Iimura

Kato

2006

Journal of Colloid and Interface Science

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Section: Interactions Of L-arg With Ddp Monolayersmentioning

confidence: 99%

Interactions of l-arginine with Langmuir monolayers of di-n-dodecyl hydrogen phosphate at the air–water interface

Hossain

Iimura

Kato

2006

Journal of Colloid and Interface Science

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“…The cytoskeleton is important in cell functions and embryonic development [ 16 ]; it gives support to the cell membrane, helps evenly split up chromosomes during cell division, and is also involved in organelle trafficking, which is the movement of cell components [ 17 , 18 ]. The cytoskeleton has the ability to alter the membrane [ 19 ] and provides a basis for movement and cell division [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Nicotine and Tocotrienol-Rich Fraction Supplementation on Cytoskeletal Structures of Murine Pre-Implantation Embryos

Hamirah

Kamsani

Khan

et al. 2017

Med Sci Monit Basic Res

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BackgroundCytoskeletal structures, in particular actin and tubulin, provide a fundamental framework in all cells, including embryos. The objective of this study was to evaluate the effects of nicotine, which is a source of oxidative stress, and subsequent supplementation with Tocotrienol-rich fraction (TRF) on actin and tubulin of 2- and 8-cell murine embryos.Material/MethodsThirty female Balb/C mice were divided into 4 groups: Group 1 received: subcutaneous (sc) injection of 0.9% NaCl; Group 2 received sc injection of 3.0 nicotine mg/kg bw/day; Group 3 received 3.0 sc injection of nicotine mg/kg bw/day +60 mg/kg bw/day TRF; and Group 4 received 60 sc injection of TRF mg/kg bw/day for 7 consecutive days. The animals were superovulated with 5 IU PMSG followed by 5 IU hCG 48 h later. Animals were cohabited with fertile males overnight and euthanized through cervical dislocation at 24 h post coitum. Embryos at the 2- and 8-cell stages were harvested, fixed, and stained to visualize actin and tubulin distributions by using CLSM.ResultsResults showed that at 2-cell stage, actin intensities were significantly reduced in the nicotine group compared to that of the control group (p<0.001). In Group 3, the intensity of actin significantly increased compared to that of the nicotine group (p<0.001). At 8-cell stage, actin intensity of the nicotine group was significantly lower than that of the control group (p<0.001). The intensities of actin in Group 3 were increased compared to that of nicotine treatment alone (p<0.001). The same trend was seen in tubulin at 2- and 8-cell stages. Interestingly, both actin and tubulin structures in the TRF-treated groups were enhanced compared to the control.ConclusionsThis study suggests that TRF prevents the deleterious effects of nicotine on the cytoskeletal structures of 2- and 8-cell stages of pre-implantation mice embryos in vitro.

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“…It is capable of modifying membrane structure [7], altering shape or morphology of the membrane [8,9], changing the mechanical properties of the cell [10], and influencing the membrane water transport [11,12]. Cytoskeletal alterations in cryopreserved embryos are attributed to many confounding factors such as developmental stage during cryopreservation, the origin of embryos ( in vivo or in vitro ), the type of cryoprotectant, selection of cryopreservation methods, and degree of zona absence [13].…”

Section: Introductionmentioning

confidence: 99%

Cytoskeletal alterations in different developmental stages of in vivo cryopreserved preimplantation murine embryos

Dasiman

Rahman

Othman

et al. 2013

Med Sci Monit Basic Res

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BackgroundThis study aimed to investigate the effects of vitrification and slow freezing on actin, tubulin, and nuclei of in vivo preimplantation murine embryos at various developmental stages using a Confocal Laser Scanning Microscope (CLSM).Material/MethodsFifty female mice, aged 4–6 weeks, were used in this study. Animals were superovulated, cohabitated overnight, and sacrificed. Fallopian tubes were excised and flushed. Embryos at the 2-cell stage were collected and cultured to obtain 4- and 8-cell stages before being cryopreserved using vitrification and slow freezing. Fixed embryos were stained with fluorescence-labelled antibodies against actin and tubulin, as well as DAPI for staining the nucleus. Labelled embryos were scanned using CLSM and images were analyzed with Q-Win software V3.ResultsThe fluorescence intensity of both vitrified and slow-frozen embryos was significantly lower for tubulin, actin, and nucleus as compared to non-cryopreserved embryos (p<0.001). Intensities of tubulin, actin, and nucleus in each stage were also decreased in vitrified and slow-frozen groups as compared to non-cryopreserved embryos.ConclusionsCryopreservation of mouse embryos by slow freezing had a more detrimental effect on the actin, tubulin, and nucleus structure of the embryos compared to vitrification. Vitrification is therefore superior to slow freezing in terms of embryonic cryotolerance.

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Effect of the polar headgroup of phospholipids on their interaction with actin

Cited by 12 publications

References 36 publications

Interactions of l-arginine with Langmuir monolayers of di-n-dodecyl hydrogen phosphate at the air–water interface

Interactions of l-arginine with Langmuir monolayers of di-n-dodecyl hydrogen phosphate at the air–water interface

Effects of Nicotine and Tocotrienol-Rich Fraction Supplementation on Cytoskeletal Structures of Murine Pre-Implantation Embryos

Cytoskeletal alterations in different developmental stages of in vivo cryopreserved preimplantation murine embryos

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