Atom probe tomography was utilized to investigate Cu precipitation in a high-strength low-alloy steel isothermally aged at 500°C for 1, 4, 16, and 64 h after water-quenching from 900°C. With prolonged aging time, the Curich precipitates (CRPs) increased in size and decreased in number density, and gradually evolved from spheroidal to elliptical in morphology. The small CRPs were rich in a high amount of Fe and a certain amount of Ni and Mn at their early nucleation stage. The large CRPs with increased size due to extensive aging contained less Fe and more Cu at their later growth stage. Additionally, Ni and Mn were both readily to segregate at the CRP/matrix heterophase interfaces, and Mn was higher in content than Ni in the precipitate interior especially when the CRPs were large in size. KEY WORDS: High-strength low-alloy steel; Thermal aging; Cu-rich precipitate; Atom probe tomography * 400°C [7-13]. Therefore, it is important to tailor the precipitation morphology of Cu-rich precipitates (CRPs) for attaining desired strength and toughness balance. Actually, the characteristics of CRPs in aspect of size, number density, shape, etc., that determine final mechanical properties are largely dependent of the compositional and resultant structural evolution during heat treatment process, and the segregation of Ni and Mn at the precipitate/matrix interface significantly prohibits the growth of CRPs to large size [14-21]. In additional, the compositional evolution of CRPs provides important information for composition modification, processing optimization, and property improvement of Cu-containing steels. However,