Effect of the Refractive Index of Buffer Solutions in Evanescent Optical Biosensors

Diéguez, Lorena; Darwish, Nawaf Bin; Mir, Mònica; Martínez, Elena; Moreno, Mauricio A.; Samitier, J.

doi:10.1166/sl.2009.1161

Cited by 64 publications

(32 citation statements)

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“…In each experiment, suspensions of NDs (100 μ g ml −1 ) were prepared in Milli-Q water or Dulbecco's Modified Eagle Medium (DMEM, Invitrogen, USA) supplemented with 5% Fetal Bovine Serum (FBS, Invitrogen, USA). For each analysis, four replicates were performed for a total of 150 measurements, at 20 °C and considering a 1.33 refractive index for water and a 1.34 for DMEM respectively [19]. …”

Section: Methodsmentioning

confidence: 99%

Combinatorial approaches to evaluate nanodiamond uptake and induced cellular fate

et al. 2016

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Nanodiamonds (NDs) are an emerging class of engineered nanomaterials that hold great promise for the next generation of bionanotechnological products to be used for drug and gene delivery, or for bio-imaging and biosensing. Previous studies have shown that upon their cellular uptake, NDs exhibit high biocompatibility in various in vitro and in vivo set-ups. Herein we hypothesized that the increased NDs biocompatibility is a result of minimum membrane perturbations and their reduced ability to induce disruption or damage during cellular translocation. Using multi-scale combinatorial approaches that simulate ND-membrane interactions, we correlated NDs real-time cellular uptake and kinetics with the ND-induced membrane fluctuations to derive energy requirements for the uptake to occur. Our discrete and real-time analyses showed that the majority of NDs internalization occurs within 2 h of cellular exposure, however, with no effects on cellular viability, proliferation or cellular behavior. Furthermore, our simulation analyses using coarse-grained models identified key changes in the energy profile, membrane deformation and recovery time, all functions of the average ND or ND-based agglomerate size. Understanding the mechanisms responsible for ND-cell membrane interactions could possibly advance their implementation in various biomedical applications.

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Section: Methodsmentioning

confidence: 99%

Combinatorial approaches to evaluate nanodiamond uptake and induced cellular fate

et al. 2016

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“…The The refractive index (RI) of the sensing medium changes due to submersing probe DNA (p-DNA) in the sensing medium. The changed refractive index (n s a ) of sensing medium after adsorption of DNA molecules is mathematically expressed as [22,23]…”

Section: Numerical Modeling Of the Proposed Sensor And Experimental Rmentioning

confidence: 99%

“…The RI increment parameter ( dn dc ) is¼0.153 cm 3 /gm in the case of water [22,23]. At a fixed incident angle if the wavelength is varied then SPR condition is achieved in which reflectance (R) of reflected wave is minimum.…”

Section: Numerical Modeling Of the Proposed Sensor And Experimental Rmentioning

confidence: 99%

Graphene coated fiber optic surface plasmon resonance biosensor for the DNA hybridization detection: Simulation analysis

Shushama

Rana

Inum

et al. 2017

Optics Communications

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“…The upper cladding of the sensing unit MR is the analyte. Here the phosphate-buffered saline (PBS) is chosen as the upper cladding, which is the common buffer solution for optical biosensors [40]. The upper cladding of the interrogation unit MZI is the amorphous perfluorinated polymer Cytop (Asahi Glass Co. Ltd.) [41], which has a similar refractive index of aqueous solutions and is propitious to realize the temperature independent waveguide biosensor chip.…”

Section: Waveguide Design For Single Mode and Polarization Independencementioning

confidence: 99%

Athermal optical waveguide microring biosensor with intensity interrogation

Han

Shao

Han

et al. 2015

Optics Communications

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Effect of the Refractive Index of Buffer Solutions in Evanescent Optical Biosensors

Cited by 64 publications

References 0 publications

Combinatorial approaches to evaluate nanodiamond uptake and induced cellular fate

Combinatorial approaches to evaluate nanodiamond uptake and induced cellular fate

Graphene coated fiber optic surface plasmon resonance biosensor for the DNA hybridization detection: Simulation analysis

Athermal optical waveguide microring biosensor with intensity interrogation

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