Effect of the Samples’ Surface With Complex Microscopic Geometry on 3 × 3 Mueller Matrix Measurement of Tissue Bulks

Abstract

The clinical in vivo tissue bulks’ surface is always coarse and shows a complex microscopic geometry which may affect the visual effect of polarization images and calculation of polarization parameters of the sample. To confirm whether this effect would cause identification difficulties and misjudgments on the target recognition when performed the polarization imaging based on 3 × 3 Mueller matrix measurement, cylindrical type and slope type physical models were used to study and analyze the effect of the surface with complex microscopic geometry on the polarization images. Then, clinical tumor bulk samples were used to interact with different sizes of patterns to simulate the different complex microscopic geometry and test the coarse surface effect on polarization images. Meanwhile, assessment parameters were defined to evaluate and confirm the variation between two polarization images quantitatively. The results showed that the polarization imaging of the sample surface with the complex microscopic geometry led to acceptable visual effect and limited quantitative variation on the value of polarization parameters and assessment parameters, and it caused no identification difficulties on target recognition, indicating that it is feasible to apply the polarization imaging based on 3 × 3 Mueller matrix measurement on clinical in vivo tissues with the complex microscopic geometry sample surface.

Backscattering Mueller Matrix polarimetry on whole brain specimens shows promise for minimally invasive mapping of microstructural orientation features

“…As anticipated, measuring non-flat geometries, such as with whole brain specimens, is particularly challenging and may introduce artifacts into the results. While other groups have been able to image non-flat surface geometries with minimal negative effects on their results (Rodríguez-Núñez et al, 2021b;Liu et al, 2022), they used wide field imaging which may have a larger depth of field than the microscopy system used in this study. To account for the limitations of this system, further studies utilizing tissue phantoms will be undertaken to characterize how curved surfaces may influence the acquisition and reconstruction of Mueller Matrix data.…”

Reflectance full Mueller matrix polarimetry for microstructural validation of diffusion magnetic resonance imaging

“…9 Additionally, though the retardance response of fiber inclination is well understood for transmittance PLI, 1,7 for reflectance PLI systems the effects have not been thoroughly investigated yet. Similarly while surface topology has been studied previously 8,16 the effects of fibers curving out of plane and the ability of reflectance PLI to map those has yet to be investigated. A better understanding of how PLI systems respond to these microstructures would be advantageous to moving the technique away from flat sectioned samples towards in vivo three-dimensional brain imaging.…”

Evaluating backscattering polarized light imaging microstructural mapping capabilities through neural tissue and analogous phantom imaging

“…Similarly, the effects of surface topology have been studied previously by Rodriguez et al who tilted and rotated fresh calf brain samples to determine this did not diminish their ability to map the optical axis of the sample 2 and Liu et al who used a reflectance based polarmetric system to image various types of tumor samples and found that surface geometry had a minimal effect on distinguishing between tumor and healthy tissue. 27 However, the effects and quantification of fibers and tissue curving out of plane on polarization properties as a whole and the ability of backscattering PLI to map those has yet to be thoroughly investigated. A better understanding of how PLI systems respond to these different microstructural arrangements is essential to advance the technique away from flat sectioned samples towards in vivo imaging of threedimensional brain structures.…”

“…who tilted and rotated fresh calf brain samples to determine this did not diminish their ability to map the optical axis of the sample 2 and Liu et al. who used a reflectance based polarmetric system to image various types of tumor samples and found that surface geometry had a minimal effect on distinguishing between tumor and healthy tissue 27 . However, the effects and quantification of fibers and tissue curving out of plane on polarization properties as a whole and the ability of backscattering PLI to map those has yet to be thoroughly investigated.…”