Effect of the substance P antagonist spantide on adrenal sympathetic nerve activity in rats.

Togashi, Hiroko; Yoshioka, Mitsuhiro; Minami, Masaru; Shimamura, Keiichi; Saito, Hideya; Kitada, Chieko; Fujino, Masahiko

doi:10.1254/jjp.43.253

Cited by 7 publications

(1 citation statement)

References 29 publications

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“…Spantide, [D-Arg', D-Trp7 9, Leu"] substance P. is one of the most potent tachykinin antagonists (Rosell et al, 1983;Folkers et al, 1984), and has often been used to examine possible neurotransmitter roles of tachykinins in peripheral and central nervous systems (Folkers et al, 1984;Fujino et al, 1987;Togashi et al, 1987;Otsuka & Yanagisawa, 1988;Barth6 et al, 1989). Several studies suggested that spantide is an antagonist with preference for NK1-tachykinin receptors (Chahl, 1985;Buck & Shatzer, 1988;Bartho et al, 1989).…”

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confidence: 99%

Pharmacological profile of a tachykinin antagonist, spantide, as examined on rat spinal motoneurones

Yanagisawa

Otsuka

1990

British J Pharmacology

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1 The pharmacological profile of a tachykinin antagonist, [D-Arg1, D-Trp7'9, Leu"] substance P (spantide), was studied on motoneurones of the isolated spinal cord of the newborn rat. For this purpose, potentials were recorded from a lumbar ventral root extracellularly and drugs were bath-applied in the presence of tetrodotoxin (TTX). 2 Neurokinin A (NKA), a NK2-receptor selective agonist, induced concentration-dependent depolarizations, which were antagonized by spantide. Analyses of concentration-response curves suggested a competitive type antagonism with a pA2 of 6.5.3 Depolarizations induced by acetyl-Arg6-septide, a NK,-receptor selective agonist, were also antagonized by spantide with a pA2 of 6.5.4 Spantide (0.5-16 pM) had no depolarizing action on the ventral root in the presence of TTX.5 Spantide antagonized the depolarizing action of substance P (SP) when SP was applied at low concentrations (0.1-0.3 pM) or by short duration pulses in artificial cerebrospinal fluid containing TTX, but much higher concentrations of spantide (4-10UM) were needed to exert an antagonistic action against SP than against acetyl-Arg6-septide or NKA. 6 Thyrotrophin-releasing hormone, L-glutamate, GABA, and noradrenaline, also induced depolarizations of the ventral root in the presence of TTX but the responses to these agonists were not depressed by spantide (16pM). 7 These results suggest that there is a subtype of tachykinin receptors on neonatal rat spinal motoneurones to which NKA, acetyl-Arg6-septide and spantide bind competitively with high affinity. The present results also suggest the existence on rat motoneurones of another class or other classes of tachykinin receptors that are less sensitive to the antagonistic action of spantide.

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Section: Introductionmentioning

confidence: 99%

Pharmacological profile of a tachykinin antagonist, spantide, as examined on rat spinal motoneurones

Yanagisawa

Otsuka

1990

British J Pharmacology

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Tachykinin antagonists inhibit the morphine withdrawal response in guinea-pigs

Johnston

Chahl

1991

Naunyn-Schmiedeberg's Arch Pharmacol

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This study was an investigation of the effects of the tachykinin antagonists, spantide and (D-Pro4, D-Trp7,9,10)substance P 4-11, injected intracerebroventricularly (ICV), on the locomotor and behavioural responses of guinea-pigs to substance P (SP) injected ICV and to naloxone-induced morphine withdrawal. SP, 50 nmol, produced increased locomotor activity and behaviour that mimicked the response induced by injection of naloxone hydrochloride, 15 mg/kg, in guinea-pigs treated 2 h previously with morphine sulphate, 15 mg/kg. Spantide or (D-Pro4, D-Trp7,9,10)SP4-11, 10 nmol, reduced the locomotor and behavioural responses to SP and to morphine withdrawal. The results support the suggestion that SP or a related tachykinin might be a mediator of the opioid withdrawal response in the central nervous system as has been proposed for the enteric nervous system.

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