Effect of themicrobial lipopeptide on tumor cell lines: apoptosis induced by disturbing the fatty acid composition of cell membrane

Abstract

Microbial lipopeptides play an important role in apoptosis induction of tumor cells. However, there is little knowledge about the relationship between apoptosis induction and membrane fatty acids. The present study focused on the effects of lipopeptides produced by Bacillus subtilis HSO121 on Bcap-37 cell lines. 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl (MTT) colorimetric assay and surface tension measurements, showed that the critical micelle concentration (CMC) was a critical level for the inhibitory activity of lipopeptides on the growth of Bcap-37 cells. Under the CMC, the order of least to greatest cytotoxicity effect on cancer cell lines by lipopeptides is C 13 -lipopeptide < C 14 -lipopepitde < C 15 -lipopeptide. Above CMC, all lipopeptides directly exert cytolytic activity. The flow cytometric analysis and Hoechst33258 staining experiments confirmed the apoptosis of Bcap-37 cell lines induced by lipopeptides in a dose-dependent manner. This apoptosis was associated with a significant decrease of the unsaturated degree of the cellular fatty acids of Bcap-37 cell lines due to the changes in the cellular fatty acids composition induced by the lipopeptide treatment. These results indicated that disturbance of the cellular fatty acid composition of breast cancer cell lines were related to in the cell apoptosis. Furthermore, significant difference in IC 50 values of tumor cells and normal cell showed that the lipopeptide exerted selective cytotoxicity on the cancer cells. Thus HSO121 lipopeptides may have potential applications as an anticancer leads.

Immunomodulatory Role of Microbial Surfactants, with Special Emphasis on Fish

“… Leukemia: B. subtilis natto T-2 derived surfactin exhibited toxicity in human K562 leukemia cells at different concentrations (2–62 μM) for 24–48 h. Surfactin inhibited the growth of K562 cells in the 24–48 h treatment period at IC 50 values ranging between 10–20 μM [ 52 ]. Hepatocellular carcinoma: The cytotoxic effect of surfactin on hepatocellular carcinoma was investigated and surfactin-like lipopeptides were found to strongly inhibit the cell viability of human Bel-7402 hepatoma cells, with IC 50 of 35 ± 12 μM [ 53 ]. Wang et al [ 54 ] found that surfactin induced apoptosis in the HepG2 cells via the ROS/ERS/Ca 2+ -mediated ERK pathway.…”

“…Wang et al [ 54 ] found that surfactin induced apoptosis in the HepG2 cells via the ROS/ERS/Ca 2+ -mediated ERK pathway. Cervical cancer: Lui et al [ 53 ] investigated the effect of surfactin on cervical cancer in HeLa cell line. The IC 50 dose of surfactin at 16–48 h was ranged between 86.9–50.2 µM [ 53 ].…”

“… Cervical cancer: Lui et al [ 53 ] investigated the effect of surfactin on cervical cancer in HeLa cell line. The IC 50 dose of surfactin at 16–48 h was ranged between 86.9–50.2 µM [ 53 ]. …”

“…Hepatocellular carcinoma: The cytotoxic effect of surfactin on hepatocellular carcinoma was investigated and surfactin-like lipopeptides were found to strongly inhibit the cell viability of human Bel-7402 hepatoma cells, with IC 50 of 35 ± 12 μM [ 53 ]. Wang et al [ 54 ] found that surfactin induced apoptosis in the HepG2 cells via the ROS/ERS/Ca 2+ -mediated ERK pathway.…”

Surfactina: estrutura, aplicações e fatores envolvidos em sua produção

“…A surfactina inibiu as vias de sinalização fosfatidilinositol-3--quinase/serina-treonina quinase (PI3K/Akt) e proteína quinase ativada (MAPK) em células MCF-7 e reduziu a insaturação do ácido graxo na membrana da célula que conduz a apoptose celular em células de câncer de mama Bcap-37. 38 A surfactina purificada do B. natto foi relatada pela primeira vez por possuir efeitos antitumorais em células do carcinoma de Ehrlich, e a surfactina purificada a partir da linhagem TK-1 inibiu células do câncer de mama MCF-7. A surfactina induziu a apoptose, e o ciclo celular parou na fase G 2 /M em células de câncer de mama MCF-7 como resultado da clivagem da caspase-3 em células MCF-7.…”