Effect of Therapeutic Ultrasound on Fibroblast Proliferation In Vitro

Sandoval, Rosy Paola Cárdenas; Malagón-Romero, Dionisio; Grazón-Alvarado, D. A.

doi:10.1007/978-3-319-13117-7_56

Cited by 2 publications

(2 citation statements)

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“…Biological responses due to mechanotransduction processes were promoted (e.g., increasing the secretion of growth factors, collagen synthesis, or calcium uptake). This was in concordance with previously reported works [ 14 , 23 , 24 , 25 , 26 , 27 , 29 , 48 , 49 ].…”

Section: Discussionsupporting

confidence: 94%

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Effects of Non-thermal Ultrasound on a Fibroblast Monolayer Culture: Influence of Pulse Number and Pulse Repetition Frequency

Peñacoba

Gutiérrez

Barrio

et al. 2021

Sensors

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Despite the use of therapeutic ultrasound in the treatment of soft tissue pathologies, there remains some controversy regarding its efficacy. In order to develop new treatment protocols, it is a common practice to carry out in vitro studies in cell cultures before conducting animal tests. The lack of reproducibility of the experimental results observed in the literature concerning in vitro experiments motivated us to establish a methodology for characterizing the acoustic field in culture plate wells. In this work, such acoustic fields are fully characterized in a real experimental configuration, with the transducer being placed in contact with the surface of a standard 12-well culture plate. To study the non-thermal effects of ultrasound on fibroblasts, two different treatment protocols are proposed: long pulse (200 cycles) signals, which give rise to a standing wave in the well with the presence of cavitation (ISPTP max = 19.25 W/cm2), and a short pulse (five cycles) of high acoustic pressure, which produces a number of echoes in the cavity (ISPTP = 33.1 W/cm2, with Pmax = 1.01 MPa). The influence of the acoustic intensity, the number of pulses, and the pulse repetition frequency was studied. We further analyzed the correlation of these acoustic parameters with cell viability, population, occupied surface, and cell morphology. Lytic effects when cavitation was present, as well as mechanotransduction reactions, were observed.

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Section: Discussionsupporting

confidence: 94%

“…Changes produced in the cells include membrane disruption, opening of reversible or irreversible pores, ion channels, and toxicity. Effects in the cells below the cavitation threshold, including proliferation, substance uptake, cell signaling, or motility increase, have also been reported [ 23 , 24 , 25 , 26 , 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%