Effect of thermal stress on HSP90 expression of Bali cattle in Barru district, South Sulawesi

Abstract

Morphometric study of mantis shrimp Harpiosquilla harpax (De Haan, 1844) Abstract. Heat shock protein 90-kDa is induced stress protein that expressed in response to stress and play crucial roles in environmental stress tolerance and adaptation. This study aimed to determine effect of environmental heat stress on the HSP90 expression of Bali cattle. Heat stress was measured by temperature humidity index in the morning and evening across 5-days on August 2016. The blood samples of Bali cattle were taken from venous jungularis. HSP90 was derived from RNA isolation of whole blood then was followed reverse transcription two steps. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was performed to analyze the transcript variants of HSP90, followed by comparative ∆∆Ct to determine HSP90 expression. The results of temperature and humidity index (THI) measurement indicated THI on afternoon was higher than in the morning. The difference in environmental conditions in the morning and afternoon effected changes on rectal temperature but neither did on Hsp90 expression.

Identification of Polymorphism within Exon 8 of Bovine HSP90AA1 Gene using PCR-SSCP Technique

“…Though there are many HSP genes, thermo tolerance in livestock animals is primarily associated with the HSP70 and HSP90 genes. The most common and temperature-sensitive is HSP90, which is thought to play an important role in environmental stress and thermal adaptation (Gade et al, 2010;Aritonang et al, 2017;Puteri et al, 2018). In summer, higher levels of expression of proteins of the HSP70 and HSP90 families have been observed in a variety of livestock species (Archana et al, tolerance, milk production, fertility, and disease susceptibility in livestock (Shergojry et al, 2014a;Kumar et al, 2015;Bhat et al, 2016).…”

Análise de parâmetros fisiológicos e perfil de expressão de genes associados ao estresse térmico e eficiência alimentar em bovinos das raças Nelore e Caracu

“…Responses to thermal stress are enhanced in Bos taurus indicus breeds, which are better able to regulate their metabolism in response to heat stress as compared to Bos taurus taurus breeds . A study performed on Bali beef cattle (Bos sondaicus) found that differences in environmental conditions in the morning and afternoon did not affect hsp90 expression (Aritonang et al, 2017). observed that HSP90AA1 (hsp90) gene expression was significantly higher during heat stress at 45 °C in Sahiwal (Bos taurus indicus) and Frieswal (Bos taurus indicus × Bos taurus taurus crossbreed) breeds in comparison to the heat stress at 39 °C.…”